过表达AtNEK6转基因烟草的耐旱耐盐性研究

doi:10.13304/j.nykjdb.2015.275

中国农业科技导报 ›› 2015, Vol. 17 ›› Issue (6): 29-36.DOI: 10.13304/j.nykjdb.2015.275

过表达AtNEK6转基因烟草的耐旱耐盐性研究

翟红红1,2,孟志刚1,张锐1,孙国清1,孟钊红1,李琴1,郭三堆1*

(1.中国农业科学院生物技术研究所, 北京 100081|2.中国农业科学院棉花研究所, 河南安阳 455000)

收稿日期:2015-05-14 修回日期:2015-07-23 出版日期:2015-12-15 发布日期:2015-12-15
通讯作者: 郭三堆,研究员,博士,研究方向为植物分子生物学与基因工程。E-mail: guosandui@caas.cn
作者简介:翟红红,硕士研究生,研究方向为植物分子生物学与基因工程。E-mail:zhai1112228@163.com。
基金资助:
国家转基因生物新品种培育重大专项（2012ZX08005-004）资助。

Overexpression of AtNEK6 Gene Improves Drought and Salt Tolerance in Transgenic Tobacco

ZHAI Hong-hong1,2, MENG Zhi-gang1, ZHANG Rui1, SUN Guo-qing1, MENG Zhao-hong1, LI Qin1, GUO San-dui1*

(1.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081|2.Cotton Research Institute, CAAS, Henan Anyang 455000, China)

Received:2015-05-14 Revised:2015-07-23 Online:2015-12-15 Published:2015-12-15

摘要/Abstract

摘要：

NEK6基因编码一种丝/苏氨酸蛋白激酶,具有调控植物细胞周期蛋白基因表达和乙烯信号转导的功能,与植物抵抗逆境胁迫相关。将拟南芥AtNEK6基因导入烟草,利用甘露醇和NaCl模拟干旱和盐胁迫环境对T2代转基因烟草进行研究。结果表明,在无胁迫处理条件下,转基因烟草根长、侧根数和鲜重显著高于野生型；在干旱和盐胁迫条件下,转基因烟草根长、侧根数和鲜重均极显著高于野生型,表现出较强的耐旱耐盐性；胁迫处理的转基因烟草叶片叶绿素荧光参数值(Fv/Fm)、SOD和CAT活性以及Pro含量极显著高于野生型,MDA含量极显著低于野生型,进一步证明了AtNEK6基因能够促进烟草的生长和提高转基因烟草对于干旱与盐碱的抵抗能力。

关键词: AtNEK6, 烟草, 生长势, 耐旱性, 耐盐性

Abstract:

NEK6 gene encodes a serine/threonine protein kinase that regulates the expression of cell cyclin related genes and ethylene signal transduction, which related to plant stress resistance. In this study, AtNEK6 over-expressed plasmid was constructed and introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco lines of T2 generation were recruited and treated with the mannitol and NaCl treatments, which simulating drought and salt conditions. Under normal condition, root length of transgenic plants was longer than that of wild type. Moreover, the lateral root number and leaf fresh weight were also much more than those in wild type. Under the stress conditions, transgenic plants showed enhanced tolerance to drought and salt stresses. The chlorophyll fluorescence parameter (Fv/Fm), SOD and CAT activities, and proline contents in leaves were increased significantly more in transgenic plants compared to those in wild type, whereas the MDA contents of transgenic lines were decreased after stress treatments, which in agreement with that obtained from stress treatments. Therefore, the transgenic tobacco was more tolerant to drought and salt stresses by over-expression of AtNEK6 gene.

Key words: AtNEK6, tobacco, growth potential, drought tolerance, salt tolerance

中图分类号:

Q786

翟红红1,2,孟志刚1,张锐1,孙国清1,孟钊红1,李琴1,郭三堆1*. 过表达AtNEK6转基因烟草的耐旱耐盐性研究[J]. 中国农业科技导报, 2015, 17(6): 29-36.

ZHAI Hong-hong1,2, MENG Zhi-gang1, ZHANG Rui1, SUN Guo-qing1, MENG Zhao-hong1, L. Overexpression of AtNEK6 Gene Improves Drought and Salt Tolerance in Transgenic Tobacco[J]. Journal of Agricultural Science and Technology, 2015, 17(6): 29-36.

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过表达AtNEK6转基因烟草的耐旱耐盐性研究

Overexpression of AtNEK6 Gene Improves Drought and Salt Tolerance in Transgenic Tobacco

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