关键词: 棉花转化;草甘膦epsps;启动子;载体构建

Abstract:

Genetically modified glyphosateresistant (GR) crops are widely commercialized GM crops in the world. However, cotton stamens are sensitive to glyphosate treatment, resulting in poor pollination and boll abortion and less cotton yield. To further improve the glyphosateresistance trait of cotton and reproductive abnormalities in GR cotton, we employed a strategy in which the optimized epsps gene from Abutilon theophrasti Medic were driven by 3 promoters of tandem coexpression, including constitutive promoter CaMV35S, cotton reproductive organs referential expression promoter arf1 and glyphosateinduced promoter ag2. The construct was introduced into cotton via Agrobacterium based flower dipping approach. Eight T0 generation glyphosateresistant plants were obtained after glyphosate screening, and their reproductive development were normal. PCR and RTPCR results suggested that the epsps gene had successfully integrated into the cotton genome and had expressed correctly. These results indicated the possibilities of broadening the expression scope about a gene promoted by different types of promoters, so as to obtaining our own genetically modified GR cotton.

Key words: cotton genetic transformation, glyphosate , epsps; , promoter; , vector construction