蜂胶中4种黄酮对HepG2细胞胰岛素抵抗的改善作用

doi:10.13304/j.nykjdb.2017.0020

中国农业科技导报 ›› 2017, Vol. 19 ›› Issue (11): 74-80.DOI: 10.13304/j.nykjdb.2017.0020

蜂胶中4种黄酮对HepG2细胞胰岛素抵抗的改善作用

梁霞丽,赵亚周,张红城,彭文君*

中国农业科学院蜜蜂研究所, 国家蜂产品加工技术研发中心, 北京 100093

收稿日期:2017-01-12 出版日期:2017-11-15 发布日期:2017-03-10
通讯作者: 彭文君，研究员，博士，研究方向为蜂产品科学。E-mail:pengwenjun@vip.sina.com
作者简介:梁霞丽，硕士研究生，研究方向为蜂产品科学。E-mail:674069856@qq.com。
基金资助:
中国农业科学院科技创新工程项目（ASTIP）资助。

Improvement Effect of Four Flavonoids from Propolis on Insulin Resistance in Vitro

LIANG Xiali, ZHAO Yazhou, ZHANG Hongcheng, PENG Wenjun*

National R&D Center for Bee Product Processing, Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China

Received:2017-01-12 Online:2017-11-15 Published:2017-03-10

摘要/Abstract

摘要： 为探究蜂胶中4种黄酮成分（高良姜素、短叶松素、松属素、柯因）对胰岛素抵抗的改善作用。通过高浓度胰岛素诱导的方式建立胰岛素抵抗HepG2细胞模型；设立正常组、模型组、高良姜素处理组、短叶松素处理组、松属素处理组、柯因处理组，测定各试验组对HepG2细胞增殖、葡萄糖消耗量、糖原含量、已糖激酶(hexokinase，HK)和丙酮酸激酶（pyruvate kinase，PK）的影响。结果显示：4种黄酮成分在有效浓度范围内对胰岛素抵抗HepG2细胞增殖均无显著影响（P>0.05）；柯因、短叶松素作用效果不明显（P>0.05）；高良姜素和松属素均能不同程度地提高IR-HepG2细胞葡萄糖消耗量（glucose consumption，GC）、肝糖原含量、HK和PK活力（P<0.05）。上述结果表明蜂胶中高良姜素和松属素能较好地调节IR-HepG2糖代谢，改善胰岛素抵抗，为蜂胶产品的深度开发提供参考。

关键词: 蜂胶, 黄酮, HepG2细胞, 胰岛素抵抗

Abstract: To determine the effect of 4 flavonoids (pinobanksin, galangin, chrysin, pinocembrin) from propolis on improvement of insulin resistance in vitro, this paper induced insulin resistance cell model by high concentration of insulin. Effects of every treatment group on cell proliferation of HepG2, glucose consumption, intracellular glycogen content, hexokinase activity and pyruvate kinase activity were determined. The results showed that different concentrations of flavonoids had no significant effect on proliferation of HepG2 cells under effective concentration(P>005). Chrysin and pinobanksin had little impact on HepG2 cell（P>0.05）. Galangin and pinocembrin could increase glucose consumption, hepatic glycogen content, HK and PK activities of IR-HepG2 cells by different degree（P<005）. These results indicated that galangin and pinocembrin could regulate glucose metabolism of HepG2 cells, and improve hepatic insulin resistance. Thus, this study provided reference for in-depth development of propolis products.

Key words: propolis, flavone, HepG2 cell, insulin resistance

梁霞丽,赵亚周,张红城,彭文君*. 蜂胶中4种黄酮对HepG2细胞胰岛素抵抗的改善作用[J]. 中国农业科技导报, 2017, 19(11): 74-80.

LIANG Xiali, ZHAO Yazhou, ZHANG Hongcheng, PENG Wenjun*. Improvement Effect of Four Flavonoids from Propolis on Insulin Resistance in Vitro[J]. Journal of Agricultural Science and Technology, 2017, 19(11): 74-80.

[1]	吴婕1,2,宫江宁3. 大孔树脂纯化金银花总黄酮及其对α-葡萄糖苷酶抑制活性的研究[J]. 中国农业科技导报, 2019, 21(10): 89-97.
[2]	曹慧方,李新新,张玥琦,石鹏君,柏映国,姚斌. 来源于脂环酸芽孢杆菌的GH1家族β-葡萄糖苷酶的葡萄糖耐受性分子改造[J]. 中国农业科技导报, 2018, 20(5): 26-33.
[3]	李彬彬,侯智霞*,杨俊枫,陈露,万如萌. ‘北陆’蓝莓叶片变色过程中类黄酮和糖的变化特性[J]. 中国农业科技导报, 2018, 20(3): 20-29.
[4]	魏娜,次顿,张唐伟. 西藏高原地理与气候因子对青稞功能性成分的影响[J]. 中国农业科技导报, 2018, 20(12): 115-121.
[5]	黄娟,邓娇,陈庆富*. 荞麦根的转录组学分析及黄酮合成基因的鉴定[J]. 中国农业科技导报, 2017, 19(2): 9-19.
[6]	熊夏宇,曾新安*,王满生,张智宏. 响应面法优化脉冲电场辅助提取油菜花粉中黄酮类物质工艺研究[J]. 中国农业科技导报, 2015, 17(5): 88-93.
[7]	苏福海1,南春实2. 蜂胶中活性成分标准物质的研制初探[J]. , 2009, 11(S1): 68-71.
[8]	张松焕,李春奇,郭惠明,裴熙祥,程红梅. 过量表达紫茎泽兰类黄酮-3’羟化酶基因对转基因烟草POD、PAL活性的影响[J]. , 2009, 11(3): 98-101.
[9]	韩正康 . 异黄酮植物雌激素调控动物神经内分泌及生产性能的研究[J]. , 1999, 1(1): 61-65.
[10]	赵亚周,田文礼,国占宝,高凌宇,彭文君. 基于响应曲面法优化蜂胶的乙醇提取工艺[J]. , 1, 1(1): 85-93.

蜂胶中4种黄酮对HepG2细胞胰岛素抵抗的改善作用

Improvement Effect of Four Flavonoids from Propolis on Insulin Resistance in Vitro

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