中国农业科技导报 ›› 2015, Vol. 17 ›› Issue (5): 88-93.DOI: 10.13304/j.nykjdb.2015.515

• 提取制备技术研究 • 上一篇    下一篇

响应面法优化脉冲电场辅助提取油菜花粉中黄酮类物质工艺研究

熊夏宇,曾新安*,王满生,张智宏   

  1. (华南理工大学轻工与食品学院, |广州 510641)
  • 收稿日期:2015-08-07 修回日期:2015-08-24 出版日期:2015-10-15 发布日期:2015-10-15
  • 通讯作者: 曾新安,教授,博士,主要从事非热加工技术及果酒果醋酿造研究。E-mail:xazeng@scut.edu.cn
  • 作者简介:熊夏宇|硕士研究生|主要从事脉冲电场的相关应用研究。E-mail:hsiungxiayu@163.com。
  • 基金资助:

    国家自然科学基金项目(21376094,21576099);广东省科技攻关项目(2012A020200002,2012A020100005);广东省科技领军人才专项(2014TX01N260)资助。

Studies on Optimization of Extraction Technology Assisted by Pulsed Electric Field of Total Flavonolds from Rape Pollen Using Response Surface Methodology

XIONG Xia-yu, ZENG Xin-an*| WANG Man-sheng, ZHANG Zhi-hong   

  1. (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China)
  • Received:2015-08-07 Revised:2015-08-24 Online:2015-10-15 Published:2015-10-15

摘要:

以油菜花粉为原料,利用脉冲电场破壁法,探讨了油菜花粉中黄酮类化合物的提取工艺。在脉冲电场强度、脉冲数、回流温度3个单因素试验的基础上,以总黄 酮类物质提取率为响应值,采用Box-Behnken响应面法设计优化了总黄酮类化合物提取的最佳工艺条件。结果表明电场强度严重影响着花粉细胞的破壁效果,且 在最优的提取工艺条件下,即电场强度28 kV/cm,脉冲数508,回流温度82℃,油菜花粉黄酮类物质提取率高达4.508%。

关键词: 油菜花粉, 总黄酮, 脉冲电场, 响应面法, 提取

Abstract:

Taking rape pollen as raw material, by using pulsed electric field wall-breaking method, this paper studied the extraction effect of total flavones from rape pollen. On the basis of testing 3 single factors——electric field intensity, pulses number and backflow temperature, taking total flavones extraction rate as response value, the best extraction technology for total flavones were optimized by Box-Behnken method. The results showed that electric field intensity had a significant effect on rape pollen cell wall breaking. Under the optimal extraction technology condition, i.e. electric field intensity of 28 kV/cm, pulses number of 508 and backflow temperature of 82℃, the total flavones extraction rate could reach 4.508%.

Key words: rape pollen, total flavones, pulsed electric field, response surface methodology, extraction

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