关键词: 响应面法, 超声提取, 三七根, 多糖

Abstract: The conditions of ultrasonic-assisted extraction (UAE) of polysaccharides from Panax notoginseng root were optimized by response surface methodology. On the basis of single factor experiments, the extraction time, ultrasonic temperature, ratio of material to liquid and ultrasonic power were selected as the influencing factors. A 4-factor, 3-level Box-Behnken central composite design (CCD) was used for response surface analysis with polysaccharide yield as response value. The results showed that the optimal conditions of UAE were as follows: ultrasonic power of 320 W, extraction time of 41 min, temperature of 58℃, and material-liquid ratio 1 g∶50 mL. Under these conditions, the polysaccharides yield of Panax notoginseng root could reach 19.51%. Compared with the traditional hot water soaking and extracting method, the extraction efficiency of UAE was obviously improved. This technology was convenient, speedness with higher productivity. It could be used to guide the industrial production of extracting polysaccharide from Panax notoginseng root.

Key words: response surface method, ultrasound-assisted extraction, Panax notoginseng root, polysaccharide