中国农业科技导报 ›› 2016, Vol. 18 ›› Issue (5): 193-199.DOI: 10.13304/j.nykjdb.2015.714

• 方法与技术创新 • 上一篇    下一篇

响应面法优化超临界提取茯苓多糖工艺研究

段丽丽,句荣辉,王辉,刘超,罗红霞*,赵新宇   

  1. 北京农业职业学院食品与生物工程系, 北京 102442
  • 收稿日期:2015-12-09 出版日期:2016-10-15 发布日期:2016-04-07
  • 通讯作者: 罗红霞,教授,博士,研究方向为食品检测与安全、畜产食品科学、食品微生物及安全。E-mail:60132@bvca.edu.cn
  • 作者简介:段丽丽,讲师,硕士,研究方向为食品生产与检验及天然物提取研究。E-mail:duanlili09@163.com。
  • 基金资助:
    北京农业职业学院技术研发与示范推广基金科研项目(XY-YF-12-20)资助。

Research of the Extraction Process of Polysaccharides from Fuling using Supercritical Method Optimized by Response Surface

DUAN Li-li, JU Rong-hui, WANG Hui, LIU Chao, LUO Hong-xia*, ZHAO Xin-yu   

  1. Department of Food and Biological Engineering, Beijing Vocational College of Agriculture, Beijing 102442, China
  • Received:2015-12-09 Online:2016-10-15 Published:2016-04-07

摘要: 茯苓多糖具有抗肿瘤、增加免疫力等多种功能,应用前景广泛,为进一步优化提取工艺,在单因素基础上,利用响应面法确定超临界CO2提取水溶性茯苓多糖的最佳工艺条件,并对其主要成分进行分析。结果表明茯苓的最佳加工工艺萃取温度50℃、萃取压力21.78 MPa、夹带剂浓度83.19%,此条件下茯苓多糖得率为3.104%,表明优选的超临界方法提取水溶性茯苓多糖优于传统方法。

关键词: 茯苓, 超临界CO2, 多糖提取, 响应面

Abstract: Based on the single factor experiments,the response surface methodology(RSM)was employed to optimize the extractions of polysaccharides from Fuling and its main ingredients were analyzed. The results of optimum extraction conditions were as follow:the extraction temperature 50℃,stress 21.78 MPa,concentration of cosolvent 83.19%. Under these conditions,the average extraction yield was 3.104%. The optimization of Fuling extraction process technology was reasonable and feasible.

Key words: Fuling, supercritical CO2, polysaccharides extraction, response surface