中国农业科技导报 ›› 2017, Vol. 19 ›› Issue (11): 92-99.DOI: 10.13304/j.nykjdb.2017.0190

• 资源环境 生物药物 生物质转化 • 上一篇    下一篇

铅胁迫下不同茶树的生理响应及其亚细胞水平铅分布特性分析

叶江华1,2,贾小丽1,3,陈晓婷3,4,林舜贤4,李远华2, 王飞权2,胡永乐1,王海斌3,4*   

  1. 1.武夷学院生态与资源工程学院, 福建省生态产业绿色技术重点实验室, 福建 武夷山 354300; 2.武夷学院茶与食品学院, 福建 武夷山 354300; 3.福建农林大学生命科学学院, 福建省农业生态过程与安全监控重点实验室, 福州 350002; 4.龙岩学院生命科学学院, 福建 龙岩 364012
  • 收稿日期:2017-04-01 出版日期:2017-11-15 发布日期:2017-06-20
  • 通讯作者: 王海斌,讲师,博士,研究方向为植物生理与分子生态学。E-mail:w13599084845@sina.com
  • 作者简介:叶江华,讲师,博士,研究方向为茶树栽培与茶叶品质机理形成。E-mail:jhye1998@aliyun.com。
  • 基金资助:
    教育部博士后科学基金项目(2016M600493);福建省自然科学基金青年基金项目(2017J05057);福建省生态产业绿色技术重点实验室开放课题基金项目(WYKF2016-6);福建省“2011协同创新中心”中国乌龙茶产业协同创新培育专项(201310);福建省中青年教师教育科研项目(JAT160504);龙岩学院人才引进项目(LB2015001)资助。

Physiological Response and Subcellular Distribution in Different Tea Plants Under Pb Stress

YE Jianghua1,2, JIA Xiaoli1,3, CHEN Xiaoting3,4, LIN Shunxian4, LI Yuanhua2, WANG Feiquan2, HU Yongle1, WANG Haibin3,4*   

  1. 1.Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, College of Ecology and Resource Engineering, Wuyi University, Fujian Wuyishan 354300; 2.College of Tea and Food Science, Wuyi University, Fujian Wuyishan 354300; 3.Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 35002; 4.College of Life Sciences, Longyan University, Fujian Longyan 364012, China
  • Received:2017-04-01 Online:2017-11-15 Published:2017-06-20

摘要: 为了分析不同茶树对Pb胁迫的生理响应及其组织亚细胞Pb分布特性,采用盆栽种植法,以铁观音、肉桂两种茶树为材料进行了分析,以期为重金属对茶树毒害机理和茶树对重金属的自我防御研究提供理论依据。结果表明:随着Pb胁迫浓度的增加,茶树叶片超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶 (peroxidase,POD)、过氧化氢酶 (catalase,CAT)活性呈现下降趋势,且肉桂对Pb胁迫的耐受性高于铁观音;相同浓度Pb胁迫下,两种茶树不同组织亚细胞中的总Pb含量显著差异;此外,Pb胁迫下,为降低Pb2+毒害,铁观音茶树表现为提高植物细胞溶质与细胞器中的Pb2+比例,肉桂茶树表现为增加植物细胞壁中的Pb2+结合率。可见,Pb胁迫下,不同茶树的解毒方式存在差异。

关键词: 茶树, 铅胁迫, 生理特性, 亚细胞, 分布特征

Abstract: In order to analyze physiological response to Pb stress and Pb distribution characteristics in subcellula tissues of different tea plants, this paper did research taking Tieguanyin and Rougui as materials by pot planting method, so as to provide theoretical basis for studying toxicity mechanism of heavy metal to tea plants and for self-defense of tea plants. The results showed that activities of leaf blade superoxide dismutase(SOD), peroxidase(POD), catalase (CAT) declined with the increase of Pb concentration, and the tolerance of Rougui to Pb stress was higher than that of Tieguanyin. There was no significant differences between the total Pb contents in subcellula tissues of the 2 tea plants under the same concentration of Pb stress. In addition, Tieguanyin increased Pb2+ propertion in cytosol and organelles to reduce Pb2+ toxicity, while Rougui mainly improved binding ratio of Pb2+. Therefore, there was different detoxification patterns for different tea plants under Pb stress.

Key words: tea plant, Pb stress, physiological characteristics, subcellular fraction, distribution characteristics