磷恢复对磷饥饿铜绿微囊藻光合色素和部分抗氧化酶活性的影响

doi:10.13304/j.nykjdb.2022.0561

摘要/Abstract

摘要：

为探究磷饥饿及磷恢复对铜绿微囊藻光合色素、藻胆蛋白和抗氧化酶等生理指标的影响，将其进行磷饥饿处理7 d后再进行磷恢复，检测磷恢复前后铜绿微囊藻的藻细胞密度、叶绿素a、类胡萝卜素、藻蓝蛋白（phycocyanin，PC）、别藻蓝蛋白（allophycocyanin，APC）、藻红蛋白（phycoerythrin，PE）、丙二醛（malondialdehyde，MDA）和过氧化氢（H₂O₂）含量及超氧化物歧化酶（superoxide dismutase，SOD）活性的变化。结果表明，铜绿微囊藻磷饥饿处理7 d后，藻细胞密度为2.54×10⁷ cell·mL^-1，显著低于对照组（3.11×10⁷ cell·mL^-1）。磷恢复144 h后，处理组藻细胞密度为4.05×10⁷ cell·mL^-1，仍显著低于对照组（4.32×10⁷ cell·mL^-1）；叶绿素a、类胡萝卜素和PC、APC、PE含量均呈现升高趋势，在144 h时分别达到5.96、1.44 μg·mL^-1和0.031、0.02、0.065 mg·L^-1；MDA、H₂O₂含量和SOD活性呈先上升后下降趋势，均在48 h达到最大值，较对照组分别增加36.2%、47.7%、51.1%。由此表明，磷恢复后铜绿微囊藻的藻细胞密度、叶绿素a、类胡萝卜素和藻胆蛋白含量虽呈升高趋势，但难以恢复到对照水平；MDA、H₂O₂含量及SOD活性的变化也说明，从磷饥饿到磷恢复后铜绿微囊藻藻细胞受到氧化损伤，并对细胞膜系统产生破坏。

关键词: 磷恢复, 磷饥饿, 铜绿微囊藻, 光合色素, 藻胆蛋白, 抗氧化酶

Abstract:

In order to explore the effects of phosphorus starvation and phosphorus recovery on the physiological indexes of Microcystis aeruginosa， its cells were treated with phosphorus starvation for 7 d and then phosphorus recovery was carried out. The cell density， the contents of chlorophyll a （chl-a）， carotenoids， phycocyanin （PC）， allophycocyanin （APC）， phycoerythrin （PE）， malondialdehyde （MDA）， hydrogen peroxide （H₂O₂） and superoxide dismutase （SOD） activity were determined. The results showed that the density of M.aeruginosa was 2.54×10⁷ cell·mL^-1， significantly lower than that of the control group （3.11×10⁷ cell·mL^-1）. After 144 h of phosphorus recovery， the algae density in the treatment group was 4.05×10⁷ cell·mL^-1， significantly lower than that of the control group （4.32×10⁷ cell·mL^-1）. The contents of chl-a， carotenoids and PC， APC， PE showed a trend of gradual increase， which were 5.96、1.44 μg·mL^-1 and 0.031， 0.02， 0.065 mg·L^-1 at 144 h. The contents of MDA， H₂O₂ and the activity of SOD all increased first and then decreased， and reached the maximum value at 48 h， which increased by 36.2%， 47.7% and 51.1%， respectively， compared with the control group. In conclusion， the cell density and the contents of Chla， carotenoid， phycobiliprotein of M. aeruginosa increased after phosphorus recovery， but it was difficult to recover to control level. The changes of MDA， H₂O₂ contents and SOD activity indicated that M.aeruginosa cells were damaged by oxidation and the cell membrane system was damaged by phosphorus starvation and phosphorus recovery

Key words: phosphorus recovery, phosphorus starvation, Microcystis aeruginosa, photosynthetic pigments, phycobili protein, antioxidant enzymes

中图分类号:

S946.3

贾滢暄, 张树林, 张达娟, 戴伟, 毕相东. 磷恢复对磷饥饿铜绿微囊藻光合色素和部分抗氧化酶活性的影响[J]. 中国农业科技导报, 2024, 26(1): 70-77.

Yingxuan JIA, Shulin ZHANG, Dajuan ZHANG, Wei DAI, Xiangdong BI. Effects of Phosphorus Recovery on Photosynthetic Pigments and Some Antioxidant Enzymes Activities of Phosphorus Starved Microcystis aeruginosa[J]. Journal of Agricultural Science and Technology, 2024, 26(1): 70-77.

图/表 6

图1 不同处理下铜绿微囊藻的生长曲线注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 1 Growth curve of M. aeruginosa under different treatmentsNote：* and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

图2 不同处理下铜绿微囊藻叶绿素a含量注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 2 Chlorophyll a content of M. aeruginosa under different treatmentsNote：* and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

图3 不同处理下铜绿微囊藻类胡萝卜素含量注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 3 Carotenoid content of M. aeruginosa under different treatmentsNote：* and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

图4 不同处理下铜绿微囊藻的藻胆蛋白含量注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 4 Phycobiliprotein contents of M.aeruginosa under different treatmentsNote： * and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

图5 不同处理下铜绿微囊藻MDA及H2O2含量注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 5 MDA and H2O2 contents of M.aeruginosa under different treatmentsNote：* and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

图6 不同处理下铜绿微囊藻SOD活性注：*和**分别表示处理组与对照组间在P<0.05和P<0.01水平差异显著。

Fig. 6 SOD activity of M. aeruginosa under different treatmentsNote：* and ** indicate significant differences between control and treatment at P<0.05 and P<0.01 levels， respectively.

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