›› 2014, Vol. 16 ›› Issue (6): 59-67.DOI: 10.13304/j.nykjdb.2014.267

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Research Progress on Positional Selectivity of Enzymatic Synthesis of Structured Lipid

LIU Si\|lei1, LIU Wei2, DONG Xu\|yan1*, WEI Fang1, LV Xin1, ZHONG Juan1, CHEN Hong1   

  1. (1.Hubei Key Laboratory of Oil Crops Lipid Chemistry and Nutrition, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062|2.College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China)
  • Online:2014-12-15 Published:2014-12-11

结构脂质位置选择性酶法合成研究进展

刘四磊1,刘伟2,董绪燕1*,魏芳1,吕昕1,钟娟1,陈洪1   

  1. (1.中国农业科学院油料作物研究所, 湖北省脂质化学与营养重点开放实验室, 武汉 430062|2.华中科技大学生命科学学院, 武汉 430074)
  • 通讯作者: 董绪燕,助理研究员,博士,研究方向为营养脂质组学与新型功能产品开发。E\|mail: dxuyan2006@163.com
  • 作者简介:刘四磊|硕士研究生|研究方向为脂质化学与营养。E\|mail:Liusilei2012@163.com。
  • 基金资助:

    国家863计划项目(2011AA10090403);国家自然科学基金项目(31171661;31271879; 21105119);湖北省自然科学基金项目(2013CFB425);武汉市科技局国际合作项目(2013030409020106)资助。

Abstract:

Fatty acids (FAs) composition, length of the carbochain and number of the double bonds in the triacylglycerols (TAGs) are key factors affecting oil and fats physicochemical proprieties and nutritional values. The location distribution of FAs in the glycerol affects the physiological functions and nutritional values of structured lipids (SLs). With the development of the functional lipids, studies on SLs received more concern. This paper summarized the influencing factors on FAs acylate position selectivity of SLs enzymatic synthesis, and laid emphasis on evaluating the catalytic specificity of lipase, reaction media and different physical assisted conditions. Meanwhile, the paper introduced the analytical and detection methods for FAs acylate position on TAG and forecasted the development prospect of SLs enzymatic synthesis on above basis.

Key words: lipase, transesterification, structured lipid, positional selectivity, reaction medium, physical assisted

摘要:

甘油三酯中脂肪酸的组成、碳链长短和双键数目是影响油脂物理化学性质及营养价值的重要因素。脂肪酸在甘油骨架上的位置分布影响结构脂质的生理功能和营养价值。随着脂质功能的开发,结构脂质的研究备受关注。综述了酶法合成结构脂质反应中的脂肪酸酰化位置选择性的影响因素,重点评价了酶促酯交换反应中脂肪酶的催化特异性、反应的环境介质及不同的物理辅助条件等因素对脂肪酸酰化位置选择性的影响,并介绍了甘油三酯中脂肪酸酰化位置的分析检测方法。在此基础上,对酶法合成结构脂质的发展前景进行了展望。

关键词: 脂肪酶;酯交换;结构脂质;位置选择性;反应介质;物理辅助

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