Abstract:

Ganoderma lucidum is an important medical fungi and has wide varieties. At present, the edible fungus management system is not yet perfect, so it is an urgent need to establish an effective way for rapid identification of Ganoderma lucidum strains to  stable strains quality. Based on SRAP (sequence\|related amplified polymorphism) analysis on cultivated strains of Ganoderma lucidum, specific SRAP marker bands belonged to several tested strains were converted into more stable SCAR (sequence characterized amplified region) markers. Seventeen SCAR markers were obtained and 23 strains were classified into 4 groups using clustering analysis under genetic distance of 0.63, and among them 19 strains were belonged to one group under the distance value of 050. Multiple markers comprehensive identification method for Ganoderma lucidum strains was established through combining usage of 9 SCAR markers, and the tested 23 strains were effectively identified. This showed that SCAR molecular markers could well explain the genetic relationship between Ganoderma lucidum strains, and it was a rapid, stable and accurate method to identify Ganoderma lucidum strains.

Key words: Ganoderma lucidum, SRAP marker, SCAR marker

摘要：

赤芝是种重要的药用真菌，品种繁多，由于目前食用菌管理制度不完善，为稳定菌株质量迫切需要建立起快速鉴定赤芝菌株的有效办法。在对赤芝（Ganoderma lucidum）菌株进行SRAP多态性分析基础上，将属于某几个菌株的SRAP特异片段转化为稳定性较高的SCAR标记，共获得17个SCAR标记。聚类分析显示，供试的23个赤芝菌株在遗传距离0.63下分为4类，其中19个菌株在遗传距离0.50下聚成一类。将其中的9个SCAR标记线性组合，建立起赤芝菌株的多标记综合鉴别法，可对供试的23个菌株进行有效鉴别。由此可见，SCAR分子标记能很好地解释赤芝菌株间的亲缘关系，是种快速、稳定、准确鉴别赤芝菌株的方法。

关键词: 赤芝；SRAP标记；SCAR标记