Abstract:

To explore the methods for recycling shrimp processing wastes with high added value, shrimp processing wastes were bio-converted by edible/medicinal fungus Cantharellus cibarius through submerged fermentation in this study. And then, the conditions of bioconversion were optimized through Placket-Burman (PB) experimental design, Path of Steepest Ascent and Response Surface Methodology (RSM) in sequence. Antihypertensive activity of the water extracted from converted products was determined through testing the inhibitory activity of the water extracts on Angiotensin I-Converting Enzyme (ACE). The results indicated that the optimized bioconversion model of Cantharellus cibariuss was established as 1∶1.857 of solid to liquid radio and 1.25% of concentration of glacial acetic acid, when the mycelium biomass and the ACE inhibitory activity of water extracts from mycelium were both kept in mind. Under this model, the mycelium biomass reached 27.837 g/L, improved by 21.231%. ACE inhibitory activity (IC50=0.394±0.071 mg/mL) of water extracts from mycelium was decreased significantly (P<0.05). The score of deodorization was calculated as 3.611±0.044. Bioconversion shrimp processing wastes by Cantharellus cibariuss had the effect of deodorization, and ACEI with relatively higher activity could be isolated from the bio-converted products.

Key words: shrimp processing wastes, bioconversion, Cantharellus cibarius, angiotensin I-converting enzyme inhibitor (ACEI)

摘要：

为探索高附加值回收利用虾加工废弃物的方法，利用鸡油菌（Cantharellus cibarius）转化虾加工废弃物，以PB设计、最陡爬坡试验和响应面设计对转化条件进行优化，以血管紧张素转换酶（ACE）抑制活性为指标考察了转化产物的抗高血压活性。结果表明，鸡油菌转化虾加工废弃物最优液体发酵条件（兼顾菌丝产量和ACE抑制活性）为料液比1∶1.857，冰醋酸加入量1.25%；对该转化条件的验证实验结果与发酵条件数学模型预测值无显著性差异（P>0.05），在此条件下菌丝体产量为（27.837±1.011） g/L，较优化前提高21.231%；菌丝体水提物ACE抑制IC50为（0.394±0.071） mg/mL，显著低于优化前（P<0.05），脱腥得分为3.611±0.044。以药用价值和经济价值较高的鸡油菌转化虾加工废弃物，其产物具有较高的ACE抑制活性，可从其中分离得到较高活性的ACE抑制剂，而且可使产物脱腥。

关键词: 虾加工废弃物, 生物转化, 鸡油菌, ACE抑制剂（ACEI）