Loading...

Table of Content

    15 February 2016, Volume 18 Issue 1
    Establishment and Application of High Efficient Breeding Technology System of Oat in China
    REN Chang-zhong*, CUI Lin*, YANG Cai, TIAN Chang-ye, FU Xiao-feng, LIU Yan-ming,
    2016, 18(1):  1-6.  DOI: 10.13304/j.nykjdb.2015.319
    Asbtract ( )   PDF (1170KB) ( )  
    Related Articles | Metrics

    In order to solve the problems existing in oat breeding, technical innovative studies on its core breeding links were carried out. The first side is to introduce and utilize foreign germplasm resources. On the basis of 300 accessions introduced from Canada, over 9 000 accessions of China-Canada hybrids oats have been breeded. The second side is to innovate a Chinese oat breeding method and establish a high effective breeding technology system in China. Using the dominant and recessive male sterile germplasm resources monopolized by our country, the sterility in tetraploid-hexapod hybrid was solved, the barrier in haploid breeding techniques of naked oat was broken, and the awn marker traits were selected. Difficult problems such as slow hybrid homozygosity, superior germplasm shortage, low excellent gene integration, narrow disease-resistant gene base, and discrimination of super marker traits have been overcome. Now, 48 new naked-oat cultivars and 12 covered-oat cultivars have been developed, including 6 national approved ones. The oat varieties are no longer of single type with high yield, but have developed into multiple types, which could meet the multi-requirements in oat growth and processing under complicated ecological environments in China.

    Progress of Eco-compensation Practice for Environmental Friendly Agriculture
    HU Bo1, YANG Ying2, WANG Qian1*, LUO Liang-guo1*
    2016, 18(1):  7-17.  DOI: 10.13304/j.nykjdb.2015.230
    Asbtract ( )   PDF (1396KB) ( )  
    Related Articles | Metrics

    Eco-compensation environmental friendly agriculture (EEFA) is an agricultural production mode, which gives consideration to both production and environmental protection. Additionally, environmental friendly agriculture possesses typical positive externalities. In developed countries, implementing eco-compensation measures to stimulate farmers to adopt environmental friendly agricultural production mode has been a practical and effective policy tool for promoting sustainable agriculture development. This paper systematically presented the practice and its effects of EEFA at home and abroad. Domestic eco-compensation practices were introduced, such as conservation tillage, soil testing and formulated fertilization, soil organic matter advancement, eco-agriculture and eco-labeling, etc. The paper also pointed out that, the main factors that restrict a more positive role of EEFA in China include compensation amount, compensation duration, and compensation modes. By learning experience from the developed countries, the paper put forward policy suggestions, such as establishing technology list for environmental friendly agriculture. All these are of important significance for speeding up the progress of EEFA in China.

    Studies on Applications of Nanomaterial and Nanotechnology in Agriculture
    SUN Chang-jiao, CUI Hai-xin*, WANG Yan, ZENG Zhang-hua, ZHAO Xiang, CUI Bo
    2016, 18(1):  18-25.  DOI: 10.13304/j.nykjdb.2015.240
    Asbtract ( )   PDF (1060KB) ( )  
    Related Articles | Metrics

    In recent years, the application of nanomaterial and nanotechnology on the field of agriculture has made some progress. This review  summarized the main progress of the applications of nanomaterials and nanotechnology in agriculture, including delivery of agricultural inputs, plant and animal breeding, agro-product processing, environment rehabilitation, nano-detection and other aspects. But at present, the application of nanotechnology in agriculture is still in its nascent stage. Some applications have been demonstrated at concept and experimental stage, and greater efforts are still required for commercialization. In addition, the characteristics of nanomaterials may cause potential safety problems due to the special nature, so it is very essential to choose environmental friendly nanomaterials and study their applications for the development of nanotechnology in agriculture.

    Research Progress on Maize Molecular Marker-assisted Breeding and Marker Development
    CHEN Xiu-hua, YU Li-juan, LUO Li-ming, CHEN Hong-mei, LIU Li*
    2016, 18(1):  26-31.  DOI: 10.13304/j.nykjdb.2015.281
    Asbtract ( )   PDF (1030KB) ( )  
    Related Articles | Metrics

    In order to fully explore and use the potential of molecular marker-assisted breeding in maize, this paper analyzed the factors influencing the efficiency of marker assisted breeding, summarized the methods for developing markers used in breeding practice, elucidated the application status of quality and resistance/tolerance breeding in maize. The paper also listed some useful tools and software to assist experiment design and analysis. Before ending, the paper thoroughly analyzed the problems existing in marker-assisted breeding, and suggested that more effort should be laid on maize gene mapping research for important agronomic traits, and the application scope of molecular marker assisted breeding should be expanded.

    OsAFC2 Involved in the Regulation of mRNA Alternative Splicing in Oryza sativa
    LIANG Meng, ZHANG Yan, XU Miao-yun, ZHANG Lan, WANG Lei*
    2016, 18(1):  32-39.  DOI: 10.13304/j.nykjdb.2015.271
    Asbtract ( )   PDF (2043KB) ( )  
    Related Articles | Metrics

    AFC2 plays an important role in the regulation of mRNA alternative splicing by phosphylating SR proteins. Up to now, the regulatory function of rice AFC2 has not been analyzed. In this study, a mutant of OsAFC2 gene was obtained by screening rice RNAi library. Further studies showed that OsAFC2 located in the nucleus and expressed in the rice various tissue, of which, OsAFC2 was highly expressed in rice leaves and low expressed in rice stem. Compared with the wild type control, the expression of OsAFC2 was obviously downregulated in the RNAi mutant and upregulated in overexpression plants. RNA-seq analysis found that the number of the alternative splicing transcripts significantly increased in OsAFC2 RNAi plants, indicating OsAFC2 involved in the regulation of mRNA alternative splicing in the nucleus.

    Construction of Plant Expression Vector of shTRAIL and its Transient Expression
    WEI Qi-chao1,2, ZHANG Rui1, MENG Zhi-gang1, SUN Guo-qing1, ZHOU Tao1, ZHAO Lei-l
    2016, 18(1):  40-45.  DOI: 10.13304/j.nykjdb.2015.287
    Asbtract ( )   PDF (1929KB) ( )  
    Related Articles | Metrics

    Human tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced apoptosis exhibits selective cytotoxicity, i.e. selectively induces apoptosis in numerous tumor cell lines while sparing normal cells, thus it has been regard as a promising candidate medicine of apoptosis-inducing therapy in malignancies. Plant bioreactor has been widely concerned due to its advantages in production of pharmaceutical proteins. Expression vector of soluble human TRAIL (shTRAIL) gene was constructed, and the Nicotiana benthamiana transient expression system was utilized in this paper. The results showed that the mean expression level of shTRAIL was 68.60 pg/mg TSP in N. benthamiana leaves, and the inhibition rate of NCI-H460 was 28.75% when treated with 200 pg/mL shTRAIL. This results indicated that the codon optimized gene could express target protein with biological activity in transgenic plants, and provide a basis for next stage of stable transformation of the target gene.

    Effects of Different Iron Concentration on Growth, Quality and Physiological Characteristics of Hydroponic Chinese Kale
    SU Wei, WANG Jian, SONG Shi-wei*, LIU Hou-cheng, CHEN Ri-yuan
    2016, 18(1):  46-52.  DOI: 10.13304/j.nykjdb.2015.285
    Asbtract ( )   PDF (2201KB) ( )  
    Related Articles | Metrics

    Dwarf phenomenon, chlorosis, low quality often occur in hydroponics kale because of iron deficiency, while the plant growth would be easily inhibited by high iron concentration due to the physiological obstacle brought about iron toxicity. In this study, the effects of iron concentrations (2.8 mg/L, 5.6 mg/L, 8.4 mg/L, 11.2 mg/L and 14 mg/L, with EDTA-Fe as a source) on the growth, quality and some physiological characteristics of hydroponic Chinese kale was investigated. Results showed that the plant fresh weight and plant height increased significantly as the iron concentration went up to 8.4 mg/L. But when the iron concentration went over 8.4 mg/L, the plant fresh weight did not increase and plant height even dropped down. It was conducive to improve nutrient contents of Chinese kale, to increase iron concentration within a certain range. The contents of soluble protein, soluble sugar and vitamin C in leaf and bolting stem would increase significantly along with the increase of iron concentration. But, the soluble protein content in leaf did not increase when the iron concentration was higher than 8.4 mg/L. The soluble protein content in bolting stem, and soluble sugar contents in leaf and bolting stem would not increase when the iron concentration was over 11.2 mg/L. Chlorophyll content, CAT and POD activities of leaf showed the tendency of first raising then dropping with the adding of iron concentration, and then reached the peak when iron concentration was 8.4 mg/L. The vitality of root system was in the same situation. Total plant iron content also increased at first and then decreased with the increase of iron concentration. The peak of total iron content in leaf was at the iron concentration of 8.4 mg/L, and the peak of total iron contents in bolting stem and root was at 11.2 mg/L. So 8.4 mg/L was considered the most appropriate iron concentration of the nutrient solution for hydroponic Chinese kale under this experimental condition.

    Effects of Medicinal Plants as Isolation Agent on Silkworm Breeding
    SHAO Yu-lan, ZHANG Yi-chuan, TANG Fen-fen, ZHONG Jian, ZHANG Yong-hong, ZHU Feng
    2016, 18(1):  53-57.  DOI: 10.13304/j.nykjdb.2015.226
    Asbtract ( )   PDF (1128KB) ( )  
    Related Articles | Metrics

    In order to find a kind of environment-friendly and disease preventive isolation agent, this experiment adopt the common medicinal plants in Yunnan mountainous areas: eucalyptus leaves, maxi leaves, pine needles as isolation agents and contrast with coke bran, the commonly used isolation agent for silkworm. Related factors affecting the fifth instar silkworm growth, such as eating mulberry condition, silkworm seat moisture absorption ability, antiviral ability, and cocoon quality were measured. The results indicated that these selected medicinal plants had no bad effect on silkworm eating mulberry leaves. When the silkworm grew to the late age of the fifth instar, the moisture absorption capacity differed obviously (P<0.05) among different treatment groups, while the same treatment group of moisture change was not obvious but significant with contrast (P<0.05). The maxi leaf group had the highest survival rate after 7 days of BmNPV infection. Compared with the coke bran, cocoon weight and cocoon shell rate of various medicinal plants had no significant differences (P>0.05). Above results shew that these common medicinal plants had no bad side-effects on silkworm, among them Artemisia argyi could replace coke bran, as a new measure for isolation in silkworm production. It is of great significance to prevent and control silkworm diseases, and reduce environment pollution.

    Studies on Immunopotentiator Improving Bivalent Vaccine Efficacy in Poultry
    LU Ji-hu1,2,3, WU Pei-pei1,3, ZHANG Xue-hua1,2,3, HOU Ji-bo1,3, TANG Ying-hua1,
    2016, 18(1):  58-65.  DOI: 10.13304/j.nykjdb.2015.272
    Asbtract ( )   PDF (1678KB) ( )  
    Related Articles | Metrics

    In order investigate the efficacy of immunopotentiator (VA5) on bivalent vaccine in chickens, the antibody levels or the challenge protection efficacy were evaluated after the specific pathogen free chickens (SPF) and commercial layers immunized with the bivalent inactivated ND-IBD vaccines with or without VA5, respectively. The other detection way was only taking SPF chicken injected by bivalent ND-AI vaccines with or without VA5, in contrast with bivalent vaccine without VA5 groups. HI antibody level against ND antigen was achieved to 6 log2 one week early in both ND contained bivalent vaccines with VA5 groups. And HI antibody level against H9 AI antigen reached to 7 log2 one week advanced in ND-AI vaccine with VA5 group. Antibody titers of agar gel precipitation titer and serum-virus neutralization against IBD antigen were qualified one week in advance than in ND-IBD vaccine with VA5 group, and continuously high antibody titer against ND, H9 AI and IBD antigen were retained during the detection periods. Similar to the antibody level results, groups of chickens injection by bivalent vaccines with VA5 could fully protect against virulent-ND or -IBD viruses challenge based on clinical signs and (or) bursa gross lesions, and no virus shedding post H9 AI variants infection. The protective efficacy of adjuvant bivalent vaccine groups was higher than those of bivalent vaccine without VA5 groups in term of the results of the three types viruses challenge. In summary, the immune efficacy of both ND-IBD and ND-AI bivalent vaccines was improved by VA5 immunopotentiators, which had shown great application prospects in this field.

    Structure and Function of ORF117 of Spodoptera litura multicapsid nucleopolyhedrovirus Ⅱ
    LIU Xing-jian, JIANG Feng, WANG Shi-bao, LI Yi-nv, ZHANG Zhi-fang, HU Xiao-yuan*
    2016, 18(1):  66-72.  DOI: 10.13304/j.nykjdb.2015.337
    Asbtract ( )   PDF (2575KB) ( )  
    Related Articles | Metrics

    The structure and function of Spodoptera litura multicapsid nucleopolyhedrovirus Ⅱ(SpltMNPVⅡ) ORF117 gene were studied. The gene and protein sequences of ORF117 (coden protein GP117) were analyzed by bioinformatics methods. The promoter activities and the time course of mRNA transcription were analyzed. Part of GP117 protein was expressed and purified to preparing the polyclonal antibody. The expression phase of ORF117 and cellular location of GP117 were analyzed using the antibody. Sequence analysis demonstrated that this gene has a 1 203 bp ORF, encoding 400 amino acids with a predicted molecular weight of 45.5 kDa and a predicted isoelectric point of 5.06. Both promoter activities analysis and the time course of mRNA transcription showed that ORF117 was a late gene. Western blotting was used to detected the gene ORF117′s expression phase and the result identified with the transcription phase. The result of cellular localization of ORF117 showed that the GP117 existed in cytoplasm more than nucleus.

    Heterologous Expression and Characterization of an Exo-polygalacturonase from Neosartorya fischeri P1
    YANG Wen-xia, LI Ke, PAN Xia, SU Xiao-yun, YANG Pei-long*
    2016, 18(1):  73-80.  DOI: 10.13304/j.nykjdb.2015.205
    Asbtract ( )   PDF (1960KB) ( )  
    Related Articles | Metrics

    Polygalacturonases has great application value in food and feed industry, so finding new polygalacturonase can not only supply related enzyme information, but also provide more choices for industrial applications. In the study, a thermophilic fungus Neosartorya fischeri P1 was isolated from an acid mine in Yunnan Province. A novel exo-polygalacturonase gene, Nf-pg, was cloned from its genome. The gene Nf-pg was transformed into Pichia pastoris GS115 for heterologous expression. The recombinant enzyme Nf-PG was purified and characterized. The results indicated that the purified Nf-PG was most active at pH 4.5 and 55℃, and showed high stability at pH 2.0~9.0 and good thermal stability. There was still 80% residual enzyme activity after 60 min heat treatment at 50℃. The optimal temperature of deglycosylated recombinant enzyme Nf-PG-D was 50℃. Compared to Nf-PG, enzyme Nf-PG-D showed a decreased thermal stability, but the properties of optimal pH and pH stability were similar to Nf-PG. Product analysis proved that Nf-PG was a strict exo-type polygalacturonase. The excellent properties made Nf-PG an ideal candidate for applying in food processing.

    Studies on Construction of Food Safety Traceability Chain
    ZHENG Huo-guo, LIU Shi-hong, HU Hai-yan
    2016, 18(1):  81-86.  DOI: 10.13304/j.nykjdb.2015.265
    Asbtract ( )   PDF (2115KB) ( )  
    Related Articles | Metrics

    The construction of food traceability system should firstly figure out what information needs to be traced, and in what way the information is organized and managed. The existing food safety traceability system is constructed in view of information system theory. Through integrated management of food quality and safety factors from food production to consumption, the construction of food safety traceability chain has become a new way of traceability system. Based on the theory of food chain, this paper decomposed the process of food chain, proposed the hierarchical model of food safety traceability chain, and analyzed its continuity and integrity. The establishment of food safety traceability chain was not only the foundation for food hazard analysis, quality control, risk analysis, but also a basis for the construction of food safety traceability system.

     

    Research on Master-slave WSN System for Fruit Canopy Micro-environment Monitoring
    GUO Xiu-ming, FAN Jing-chao, ZHOU Guo-min*, QIU Yun, HU Lin
    2016, 18(1):  87-94.  DOI: 10.13304/j.nykjdb.2015.241
    Asbtract ( )   PDF (2007KB) ( )  
    Related Articles | Metrics

    Micro-environment of a tree canopy is a key factor influencing fruit quality and yield. WSN system can be used to achieve real-time and automatic monitoring. All nodes have the same equal status in traditional WSN system, while in a fruit tree canopy, it is redundancy that all nodes have sensing, managing and data transmission capability. The high-density nodes with the same equal status makes the WSN lack of reasonable architecture and levels, thus have low performance. To develop a WSN system for fruit tree canopy micro-environments monitoring, the traditional WSN was split, recombined, and a master-slave WSN system with segmentation of sensing ability was proposed. Each canopy was monitored by one sub-system which was composed of one master node and some slave nodes. The slave nodes were in charge of sensing information, and the master node took charge of managing the slave nodes and communicating with the other master nodes to transmit data. To evaluate the performance of the new system, the hardware and software were designed, and contrast experiments were performed. The results showed that the new system doubled the life cycle, and was more effective and energy saving. At present, it is suitable for micro-environment monitoring of fruit canopies.

    Research Advance on Recommendation for Crop Fertilization Methodology
    CHUAN Li-min1, HE Ping2*, ZHAO Tong-ke3*
    2016, 18(1):  95-102.  DOI: 10.13304/j.nykjdb.2015.302
    Asbtract ( )   PDF (1205KB) ( )  
    Related Articles | Metrics

    Scientific fertilization is an important measure to improve crop yield and nutrient use efficiency. At present, unscientific fertilization, low nutrient use efficiency and low production yield are the common problems in cropping system, which have resulted in fertilizer resources wasting, environment risk increasing and agro-food quality decreasing. Studies on the theory and methods of high efficient fertilizer are of important significance for ensuring food security, improving fertilizer use efficiency and protecting eco-environment safety. According to current situation of crop fertilization and nutrient use efficiency, this paper analyzed the effects of unscientific fertilization on soil, water, air, agro-food quality and human health; summarized two recommended fertilization strategies based on soil nutrient test and aboveground crop response; and introduced soil test method, fertilizer effect function method, chlorophyll meter, leaf color chart, nitrate reflect meter, canopy reflect meter, plant symptom diagnosis and methods based on crop yield response and agronomic efficiency. The paper also suggested that a suitable fertilizer method should be selected, so as to adapt to local conditions, achieve high production yield, be friendly to environmental and secure sustainable utilization of farmland.

    Research Progress on High Efficient Water Utilization of Wheat
    RAN Wen-xing1, WANG Ji-chuan1,2*, WANG Pu2*
    2016, 18(1):  103-111.  DOI: 10.13304/j.nykjdb.2015.362
    Asbtract ( )   PDF (1247KB) ( )  
    Related Articles | Metrics

    Under limited water resources condition, and with modern sustainable agriculture development, the mechanism and restricting factors of high water use efficiency for wheat have been extensively studied, and certain achievements were gained. This paper introduced in detail the internal and external factors affecting water efficient utilization of wheat, and research progress made in studying wheat physiological characteristics for high efficient water usage, and related production measures. The paper also prospected the future research direction, hoping to provide references for efficient water utilization and improvement of wheat production.

    Development Trend of Irrigation Water Conservancy Construction in Karst Mountain Areas ——A Case of Guizhou Province
    SONG Ju-lan, LUO Hai-bo*, WU Lin-na, PAN Bo-juan, CHEN Yuan-yun
    2016, 18(1):  112-119.  DOI: 10.13304/j.nykjdb.2015.326
    Asbtract ( )   PDF (1213KB) ( )  
    Related Articles | Metrics

    Irrigation and water conservancy project is a necessary condition for agricultural production and development. But at present, there were some problems in farmland water conservancy construction in Karst mountainous areas. Therefore, it is of important and realistic significance to strengthen the studies on the construction of farmland water conservancy for developing farmland irrigation water conservancy, rational planning and layout, and increasing grain yield in Karst mountainous areas. Taking Guizhou Province as an example, this paper briefly expounded the construction and development of irrigation and water conservancy engineering during the recent years, including application of advanced technology and efficiency evaluation. Meanwhile, it also put forward problems existing in irrigation and water conservancy engineering in Karst mountainous areas, and relevant solving measures and suggestions. Before ending, the paper pointed out the development trend for farmland irrigation and water conservancy engineering is to apply new and high technology, to save water and farmland, to realize management modernization, to protect landscape and ecological condition, and to adopt 3 superb technology, etc.

    Variance Analysis on Potassium and Chlorine Contents of Flue-cured Tobacco among Different Varieties and Producing Areas in Bozhou
    JIANG Chao-qiang1,2, LI De-cheng2, WANG Huo-yan2, ZHANG Dong-qi3, SHEN Jia1, YAN
    2016, 18(1):  120-128.  DOI: 10.13304/j.nykjdb.2015.306
    Asbtract ( )   PDF (1324KB) ( )  
    Related Articles | Metrics

    In order to understand the characteristics of potassium and chlorine contents in tobacco leaves, tobacco leaves of different varieties and soil samples were collected from 2013-2014 in Bozhou, Anhui Province, and the potassium and chlorine contents and soil physical and chemical characteristics were analyzed. The results showed that the potassium content of flue-cured tobacco leaves were 6.2~21.9 g/kg. The average potassium content of upper, middle and lower part tobacco leaves were 13.9 g/kg, 13.7 g/kg, and 11.7 g/kg, respectively. The leaf potassium content in over 70% samples were lower than 15 g/kg, while that of only 50% samples were higher than 10 g/kg. Just a few sampless potassium contents were higher than 20 g/kg. There was no significant difference of potassium content in the same position of leaves between difference areas in Bozhou. The potassium content in different varieties followed the order as Zhongyan 100 > Longjiang 911 > NC 89. Chlorine content of flue-cured tobacco leaves were 4.3~20.1 g/kg in Bozhou. The average chlorine content of upper, middle and lower tobacco leaves were 10.3 g/kg, 10.2 g/kg and 9.0 g/kg, respectively. The leaf chlorine contents in Lumiao and Shuanggou were lower than that in Zhangji. There was no significant difference of potassium content in the same position of leaves between varieties in Bozhou. Generally the potassium content in Bozhou tobacco leaves was low, their chlorine content was high, and potassium/chlorine was low. Therefore, how to increase the potassium content and reduce chlorine content was crucial for improving the quality of Bozhou tobacco leaves. In order to increase potassium content of tobacco leaf, we should improve fertilization way, such as applying root zone fertilization, and increasing irrigation during drought period. On the other hand, we should choose soil with low chlorine content as tobacco producing field, forbid to use chlorine fertilizer, and implement free flooding irrigation to reduce leaf chlorine content in Bozhou.

    Correlation Analysis of Chemical Components and Physical Properties of Heavy Flavor Flue-cured Tobacco in Hunan Province
    LIU Zhi-xuan, ZHOU Qing-ming, LI Juan*, LIU Hui, CHEN Jia-liang, LU Yuan
    2016, 18(1):  129-135.  DOI: 10.13304/j.nykjdb.2015.210
    Asbtract ( )   PDF (1178KB) ( )  
    Related Articles | Metrics

    In order to explore the relationship among quality evaluation indicators of the flue-cured tobacco leaf, 257 tobacco samples in Hunan were collected from 2011-2013, the relevance between chemical components and physical properties was studied through a simple correlation and canonical correlation analysis. The results showed that most indicators of chemical compositions of Hunan flue-cured tobacco revealed extremely significant correlation, while indexes of physical properties showed significant or extremely significant correlation, except for leaf length, equilibrium moisture content and stem ratio. Chemical composition and physical properties were significantly correlated, which was associated with the correlation between total sugar and leaf length (positive), leaf width (positive), single leaf weight (negative), total nitrogen and single leaf weight (positive), weight per unit area (positive), nicotine and single leaf weight (positive), weight per unit area (positive), leaf length (positive), leaf width (negative). These results would provided some evidence to tobacco quality evaluation system.

    Effects of Topdressing Different Potassium Fertilizer on Quality Traits of Flue-cured Tobacco
    HAO Hao-hao1, XUE Li-xin2, XU Zi-cheng1*, WU Yan-hui1, XU Yi2, JIN Lei2, LI Jian
    2016, 18(1):  136-143.  DOI: 10.13304/j.nykjdb.2015.203
    Asbtract ( )   PDF (1217KB) ( )  
    Related Articles | Metrics

    In order to explore the effects of topdressing different potassium fertilizer on tobacco quality traits, by combining field experiment with laboratory analysis, and taking ‘Zhongyan 100’ as material, this paper studied the effects of topdressing different potassium fertilizer on the contents of chemical components, organic potassium index and neutral aroma constituents in flue-cured tobacco. The results showed that topdressing potassium fertilizer could improve the leaf quality of flue-cured tobacco. The effectiveness of topdressing potassium citrate fertilizer was the best, the routine application of topdressing potassium fertilizer plus biological potassium fertilizer was the second, but routine topdressing potassium fertilizer plus organic potassium fertilizer had a poor effect, similar to the conventional fertilizer. The topdressing potassium citrate fertilizer treatment and the routine application of potassium fertilizer and biological fertilizer treatment could effectively coordinate the ratio of total sugar to nicotine in upper flue-cured tobacco leaves, and obviously enhance the organic potassium index of all parts of tobacco leaves, and increase the content of aroma components of middle part of flue-cured tobacco leaves. Generally, topdressing potassium citrate fertilizer or adding more organic potassium fertilizer could effectively improve the quality of tobacco leaves. To certain extent, topdressing organic potassium fertilizer could instead the expensive potassium citrate fertilizer to improve the leaf quality of flue-cured tobacco.

    Research Progress in Forage Grass Silage Technology and Enlightenment for its Application in Rocky Desertification Areas
    XIONG Kang-ning1,2, XU Liu-xing1,2, SHEN Xiao-yun3, ZHANG Jin-hua4, LIU Cheng-mi
    2016, 18(1):  144-153.  DOI: 10.13304/j.nykjdb.2015.339
    Asbtract ( )   PDF (1078KB) ( )  
    Related Articles | Metrics

    This paper systematically reviewed the development of forage grass silage technology in the recent decades, analyzed the main achievements and the key problems from theoretical basis, technology research and development, experiment and demonstration, monitoring and evaluation the above 4 layers; prospected the future research work and pointed out the key tasks were strengthening the research on silage additives, improving resource utilization rate, expanding the range of silage technology popularization. According to the characteristics of silage animal husbandry in Karst rocky desertification areas, the paper analyzed how to apply silage grass technology to enhance the development of animal husbandry, and to offer help for harnessing rocky desertification areas.

    Relationship between Composition Characteristics of Soil Humus and Physiochemical Characteristics of Soil in Yunnan Gold Corridor Tobacco-growing Areas
    WANG Shan-shan1, LU Xiu-ping2, XU Zi-heng1*, LI Jun-ying2, PANG Tao2
    2016, 18(1):  154-163.  DOI: 10.13304/j.nykjdb.2015.409
    Asbtract ( )   PDF (2689KB) ( )  
    Related Articles | Metrics

    To study the composition characteristics of soil humus in Yunnan Golden Corridor tobacco-growing areas and its relationship with the physiochemical characteristics of soil, 58 soil samples in 10 cities of Yunnan Gold Corridor tobacco-growing areas were analyzed by cluster analysis, correlation analysis and other statistical methods. The results showed that: ① Carbon content of humus (Hu-C) in Yunnan Gold Corridor tobacco-growing areas was 15.97 g/kg in average, fulvic acid carbon content (FA-C),humic acid carbon content (HA-C) and humin carbon content (HM-C) were 3.45 g/kg, 1.72 g/kg and 10.80 g/kg, respectively. HA/FA ratios ranged from 0.19 to 2.06, with the average of 0.54, and the humification degree of humus was relatively low. ② In the 10 cities of Yunnan Golden Corridor tobacco-growing areas, the overall performance of the content of carbon and components in soil humus was higher in Honghe, Chuxiong, Baoshan and Wenshan. ③ The Hu-C of purple soil was significantly lower in four soil types, and other soil types were as follows: paddy soil> red soil> alluvial soil; HA/FA ratio was all less than 1 in four soil types, and it was as follows: alluvial soil > purplish soil> paddy soil> red soil. ④ The Hu-C was significantly negatively correlated with the content of soil sand and coarse dust, but significantly positively correlated with the content of fine clay and cation exchange capacity, the composition of soil humus was negatively correlated with the pH of soil.

    Review and Prospect on Seedling Production and Breeding Techniques of Undaria pinnatifida in China
    SHAN Ti-feng1,2, LI Jing1,2, PANG Shao-jun1,2*
    2016, 18(1):  164-170.  DOI: 10.13304/j.nykjdb.2015.338
    Asbtract ( )   PDF (1040KB) ( )  
    Related Articles | Metrics

    Undaria pinnatifida is one of the most important cash seaweeds in China. Its yield and economic value is only being second to Saccharina japonica in brown algae. Large scale cultivation of U. pinnatifida started from the 1980s. However, fundamental studies on seedling culture began earlier in the 1960s. Seedling production and breeding technique play a key role in the production industry of U. pinnatifida. Recently, U. pinnatifida seedling production and breeding technique have made rapid progress, there are still many problems needed to be resolved. This paper reviewed the development history of U. pinnatifida seedling culture and breeding technology, analyzed the present status and existing problems, and prospected its future development trend, so as to provide references for the future development of U. pinnatifida production industry.

    Condition Optimization of Bioconversion Shrimp Processing Wastes with Cantharellus cibarius for Angiotensin I-Converting Enzyme (ACE) Inhibitors
    GAO Xiu-jun, YAN Pei-sheng*
    2016, 18(1):  171-181.  DOI: 10.13304/j.nykjdb.2015.295
    Asbtract ( )   PDF (3789KB) ( )  
    Related Articles | Metrics

    To explore the methods for recycling shrimp processing wastes with high added value, shrimp processing wastes were bio-converted by edible/medicinal fungus Cantharellus cibarius through submerged fermentation in this study. And then, the conditions of bioconversion were optimized through Placket-Burman (PB) experimental design, Path of Steepest Ascent and Response Surface Methodology (RSM) in sequence. Antihypertensive activity of the water extracted from converted products was determined through testing the inhibitory activity of the water extracts on Angiotensin I-Converting Enzyme (ACE). The results indicated that the optimized bioconversion model of Cantharellus cibariuss was established as 1∶1.857 of solid to liquid radio and 1.25% of concentration of glacial acetic acid, when the mycelium biomass and the ACE inhibitory activity of water extracts from mycelium were both kept in mind. Under this model, the mycelium biomass reached 27.837 g/L, improved by 21.231%. ACE inhibitory activity (IC50=0.394±0.071 mg/mL) of water extracts from mycelium was decreased significantly (P<0.05). The score of deodorization was calculated as 3.611±0.044. Bioconversion shrimp processing wastes by Cantharellus cibariuss had the effect of deodorization, and ACEI with relatively higher activity could be isolated from the bio-converted products.

    Method Research of Quantitative Detection for Ochratoxin A by Aptasensor Combining Portable Glucose Meter
    ZHANG Yong1,2, ZHENG Nan2, WEN Fang2, ZHANG Yang-dong2, LI Fa-di1, WANG Jia-qi1,
    2016, 18(1):  182-188.  DOI: 10.13304/j.nykjdb.2015.233
    Asbtract ( )   PDF (2061KB) ( )  
    Related Articles | Metrics

    Despite years of research, most current methods for quantitative detection of small molecular toxins still require laboratory-based or customized devices that are not widely available to the general public for quantitative analysis. A novel portable biosensor for sensitive and selective detection of ochratoxin A (OTA) was developed based on the specific recognition between aptamer and OTA using a personal glucose meter (PGM) as readout. The enhanced signal of the PGM had a linear relationship with the concentration of OTA in the range of 1.0×10-8~4.0×10-6 mol/L, the detection limit was 6.7×10-9 mol/L (2.69 μg/kg). This method could be used to quantitatively detect OTA present in infant rice cereal and Chinese wildrye samples with good recoveries in the range of 84%~122%. This aptasensor provided a new method to determine OTA and displayed good potential for practical application.

    Economic Impact of Highly Pathogenic Avian Influenza on Broiler Industry in China
    HUANG Ze-ying, WANG Ji-min*
    2016, 18(1):  189-199.  DOI: 10.13304/j.nykjdb.2015.377
    Asbtract ( )   PDF (1239KB) ( )  
    Related Articles | Metrics

    Broiler industry is an important part of animal husbandry in China, but it is heavily affected by highly pathogenic avian influenza. In order to evaluate the economic loss caused by the disease, and guarantee the healthy and stable development of broiler industry, highly pathogenic avian influenzas impact parameters were introduced to partial equilibrium model regarding broilers supply and demand, to establish a model to measure the diseases economic impact on broiler production, consumption, export and import. The result showed that a highly pathogenic avian influenza outbreak would make chicken output, per capita consumption of chicken, chicken exports and imports in China reduced 0.003%, 0.01%, 0.021% and 0.049%, respectively. Highly pathogenic avian influenza in 2004 and 2005 had the most serious influence on broiler industry in China. From 2004 to 2009, the loss of broiler industry caused by highly pathogenic avian influenza was the consumption > the imports > the production > the exports. This model could help to get further understanding in economic impact of highly pathogenic avian influenza on broiler industry. This research method would also fit for studying the economic impact of natural disasters on other industries.

    Comprehensive Evaluation and Research of Agricultural Science and Technology Competitiveness of the Major Cities in Gansu Province
    JIN Ying1, CHEN Bing-pu1*, MA Yan-ling1,2
    2016, 18(1):  200-208.  DOI: 10.13304/j.nykjdb.2015.380
    Asbtract ( )   PDF (1262KB) ( )  
    Related Articles | Metrics

    Agricultural science and technology is a powerful driving force to promote transformation of traditional agriculture to modern agriculture. In recent years, the Chinese leaders have shown great concern on the innovation of agricultural science and technology and the upgrading of competitiveness in agricultural science and technology. Gansu Province located in the northwest region of China is actively carrying out the strategic task of developing modern agriculture, strengthening scientific and technological innovation capacity in rural areas, and improving the transformation rate of achievements gained in agricultural science and technology. According to the data of 12 prefecture-level cities and 2 autonomous prefectures in Gansu Province in agricultural science and technology in 2013, this paper established a comprehensive indicator system to evaluate the agricultural science and technology competitiveness. And through clustering analysis the paper divided these cities into 4 categories according to their similarities in competitiveness. The results showed that the level of agricultural science and technology competitiveness was positively correlated with the development level of total economy and agricultural economy in the region. There were gaps in the levels of agricultural science and technology development among those 4 groups. But there were also rooms for further development, which should not be overlooked. The paper provided policy suggestions for improving the competitiveness in agricultural science and technology in Gansu Province. All these would play a demonstration role for other provinces and cities in western regions of China to upgrade competitiveness in agricultural science and technology.

    Quality Evaluation Model for New Plant Variety Right based on Logistic Regression ——Taking Plant Variety Right of Hybrid Rice as a Case
    REN Jing1,2, SONG Min1,2*
    2016, 18(1):  209-216.  DOI: 10.13304/j.nykjdb.2015.346
    Asbtract ( )   PDF (1181KB) ( )  
    Related Articles | Metrics

    With the rapid increase of application for authorization of new plant variety right, the problem of "rich quantity and poor quality" has attracted wide attention. How to evaluate the quality of variety right has become an important theoretical and practical issue. In order to get scientific and rational judgment on the comprehensive quality level of plant new variety right in China, this paper firstly defined the definition of the quality of plant new variety right from the competitiveness angle; then set up the quality evaluation index system of new plant variety right from 3 attributes-legality, technicality and economy; and used the mean difference method to verify the effectiveness  of variety right quality index; finally constructed a comprehensive quality index of the plant new variety right to evaluation variety right quality by logic regression model so as to evaluate the quality of plant new variety right comprehensively and rapidly. Studies on quality evaluation of plant new variety right was not only beneficial for solving the problem in quality judgment standards for plant new variety right, providing a theoretical basis to improve the quality of plant new variety right, but also provided important references for value evaluation, transfer permission, financing, mortgage, and so on in China.