Abstract:

In order to find a kind of environment-friendly and disease preventive isolation agent, this experiment adopt the common medicinal plants in Yunnan mountainous areas: eucalyptus leaves, maxi leaves, pine needles as isolation agents and contrast with coke bran, the commonly used isolation agent for silkworm. Related factors affecting the fifth instar silkworm growth, such as eating mulberry condition, silkworm seat moisture absorption ability, antiviral ability, and cocoon quality were measured. The results indicated that these selected medicinal plants had no bad effect on silkworm eating mulberry leaves. When the silkworm grew to the late age of the fifth instar, the moisture absorption capacity differed obviously (P<0.05) among different treatment groups, while the same treatment group of moisture change was not obvious but significant with contrast (P<0.05). The maxi leaf group had the highest survival rate after 7 days of BmNPV infection. Compared with the coke bran, cocoon weight and cocoon shell rate of various medicinal plants had no significant differences (P＞0.05). Above results shew that these common medicinal plants had no bad side-effects on silkworm, among them Artemisia argyi could replace coke bran, as a new measure for isolation in silkworm production. It is of great significance to prevent and control silkworm diseases, and reduce environment pollution.

Key words: silkworm, medicinal plants, isolation agent

摘要：

为寻找一种环保且防病的隔离剂，采用云南省山区常见的药用植物，桉树叶、艾叶、松针作为隔离剂，与家蚕常用隔离剂焦糠作对照，考察家蚕食桑情况、蚕座吸湿能力、抗病毒能力、茧质成绩等因素对五龄期家蚕生长的影响。结果发现，供试粗型药用植物不影响家蚕食桑；随着家蚕生长到五龄后期，不同处理组的吸湿能力差异明显（P＜0.05），而同一处理组的吸湿变化不明显但与对照组差异显著（P＜0.05）；家蚕核型多角体病毒感染7 d后艾叶组的存活率最高；全茧量、茧层率方面各药用植物与焦糠组无显著差异（P<0.05）。上述结果表明供试药用植物对家蚕无毒副作用，艾叶可代替焦糠作为隔离剂的新措施，对防治家蚕病害、减少环境污染具有重要意义。

关键词: 家蚕, 药用植物, 隔离剂