Table of Content

15 April 2016, Volume 18 Issue 2

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Studies on Comparative Advantage and Developing Tendency of Major Potato Producing Areas in China

ZHONG Xin1, JIANG He-ping1, ZHANG Zhong-ming2*

2016, 18(2):  1-8.  DOI: 10.13304/j.nykjdb.2015.527

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Along with China taking the strategy of developing potato as a stable food, potatos role in assuring Chinas food security has been continuously upgraded. This paper analyzed the regional distribution features and comparative advantages of potato using the comprehensive comparative advantage index method and grey system prediction model. The results indicated that northwest and southwest regions were the regions with comprehensive comparative advantages for potato production in China, and potato mainly grew in northwest, southwest and northeast regions, but the production center was gradually transferred from northwest and northeast to southwest region. And each region had formed its own unique cultivation pattern. The comprehensive comparative advantage of potato production in northwest, northeast and southwest regions were rather high. Using GM (1.1) model, the advantage areas for potato production was predicted to be further concentrated to northwest and southwest regions, and the number of potato production region with comparative advantage in China would be increased within the coming 10 years.

Effects of Light Quality on Growth and Morphologenesis Regulation of Vegetables: A Review

HE Wei, YANG Zhen-chao*, CAI Hua, WANG Da-fei, WANG Xiao-xu

2016, 18(2):  9-18.  DOI: 10.13304/j.nykjdb.2015.421

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Light is an important energy source and information source for plant growth and development. Light quality as key trigerring factor in the light environment can regulate the whole life cycles of plants by light receptor signaling pathways. The control technology of light quality has been applied gradually to production process of vegetable crops, along with the studies on photobiology and constant R & D on artificial light source with low cost and high efficiency. This paper reviewed the research progress on light quality in regulating plant photosynthesis, mechanism of constructing photomorphogenesis, and application in vegetables production. The papper also introduced in detail the regulating mechanism of light quality during plants growth process, its effects on sprouts, green leafy vegetables, solanaceous fruit vegetables and edible fungi, etc. and how studies on light quality improve economic benefits of vegetables production. The paper elaborated combination schemes of light quality for different kinds of vegetables under artificial light environments, and put forward suggestions for further studying the function of light quality in each growth phase, and carrying out experiments on combining light intensity and photoperiod, so as to optimize specific light recipes for different kinds of vegetables.

Research Progress on Light Simple and Efficient Production Technology of Foxtail Millet in China

LI Shun-guo§, XIA Xue-yan§, LIU Meng, ZHAO Yu, LIU Fei,

2016, 18(2):  19-24.  DOI: 10.13304/j.nykjdb.2015.356

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With the rapid development of modern agriculture, and speeding up of land circulation, the major operators of new type enterprises have raised their demands for millet production to realize production with large scale but simplified technology, which the traditional enterprises were unable to satisfy. Therefore, in recent years, scientists have carried out R & D on light and simplified production technology. At present, breakthrough has been made in R & D progress in millet light simple efficient production technology in China. This paper summarized and analyzed the present status of this technology, including millet chemical thinning and weeding, breeding and simplified cultivation of herbicide-resistant millet, a complete set of agricultural machinery, and a combination of millet light simple efficient production scheme, etc.. The paper also conducted investigation on the application effect of this technology, and put up relative suggestions according to the existing problems, so as to provide theoretical basis for light, simple, and efficient millet production in China.

Gene Mapping of a Rolled Leaf Mutant rlm1 in Rice

SHEN Ya-jun1, CUI Xue-an2, ZHANG Zhi-guo2, WU Jin-xia2*

2016, 18(2):  25-30.  DOI: 10.13304/j.nykjdb.2015.361

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Leaf is a main organ for plant photosynthesis. Fine leaf shape is beneficial for building an ideal plant type and improving the efficiency of photosynthesis. In order to study the molecular mechanism of leaf morphogenesis, an half rolled leaf mutant rlm1 was discovered from the rice T-DNA insertion mutant library. The mature leaf of rlm1 mutant involuted along midrib, and finally become half cylinder. The leaf rolling index was 0.64, leaf erect parameter was 95, and the mutant photosynthesis efficiency was higher than the wild one. The mutation site of rlm1 was verified by mapping at the 1st intron of gene LOC_Os03g06654, which encoded flavin-containing monooxygenase. RT-PCR expresion and analysis indicated that LOC_Os03g06654 was inactive completely by T-DNA insertion. The gene was another allele of the reported rice curly leaf gene OsCOW1(Constitutively Wilted 1). Due to the good agronomic property of rlm1, it could be used to conduct high photosynthetic breeding practice.

Research Progress on Salt Tolerance Mechanisms in Quinoa

LI Li-li1, JIANG Qi-yan2*, NIU Feng-juan2, HU Zheng2, ZHANG Hui2*

2016, 18(2):  31-40.  DOI: 10.13304/j.nykjdb.2015.437

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Quinoa is a facultative halophytic plant species with high tolerant to salinity, and some varieties could tolerant salimity level as high as those present in sea water. A number of studies have been performed on the influence of salt stress on quinoa and the mechanism of salt tolerance in quinoa. This review summarized the existing knowledge of the salt tolerance of quinoa. The key traits included an efficient control of Na+ sequestration in leaf vacuoles, xylem Na+ loading, higher ROS tolerance, better K+ retention, and an efficient control over stomatal development and aperture. And the potential of quinoa for cultivation in salt-affected regions was discussed to provide the theoretically basis for quinoa being cultivated in other arid and semi-arid regions, phytoremediation of saline soils and further research in the field of plant salt tolerance.

Advances on Residues and Detection of β-Agonists in Animal Hair

ZHANG Kai, ZHANG Jun-min*, ZHAO Qing-yu

2016, 18(2):  41-46.  DOI: 10.13304/j.nykjdb.2015.388

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The illegal use of β-agonist in animal husbandry is very dangerous for animal products safety. Chinese government attaches great importance to β-agonists regulator of animal products. Most β-agonists eliminates slowly in animal hair. In addition, hair is non-invasive to take and easy to store. It has great advantage to use it as supervision target in animal husbandry. This review summarized the structure and growth pattern of hair, mechanism of how drug entering into hair, its residue elimination rule, and detection technology of β-agonists in animal hair. All these could provide scientific basis for taking animal hair as supervision target of β-agonists in animal husbandry.

Research on Microstructures and Physical Properties of Honeybee Silk

LI Shi-yi, HU Fu-liang*

2016, 18(2):  47-51.  DOI: 10.13304/j.nykjdb.2015.312

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Insect silk is not only raw material of textile silk fiber, but also a kind of biological material with bright application prospects, due to its good mechanical properties and biocompatibility.The honeybee silk and silkworm silk have different protein structures, biological properties and material properties, so each has its unique research and development value. This article described the source of the honeybee silk and its role in nest, the composition and molecular structure and other macroscopic properties, such as crystallinity, solubility, mechanical properties, etc.; compared the honeybee silk with silkworm silk in the relevant characteristics in order to provide a reference for basic research and development of utilization.

Progress on Fumagillin and its Application on Apis Nosema Disease Control

CAO Wei-rui, XUE Xiao-feng, WU Li-ming*

2016, 18(2):  52-58.  DOI: 10.13304/j.nykjdb.2015.345

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Fumagillin is a potent antibiotic isolated from Aspergillus fumigatus. It is widely used in apiculture curing Apis Nosema disease. This paper summarized the research progress made in fumagillin, including its molecular structure, antibiotic theory, toxicity, stability; the usage status in controlling Apis Nosema disease; and the detection methods of fumagillin residue in apiculture products. The paper also prospected the future research directions concerning fumagillin and its application in apiculture industry.

Site-directed Mutagenesis of Glutamate Decarboxylase and its Synthesis of Gamma Aminobutyric Acid

GAO Cui-juan

2016, 18(2):  59-64.  DOI: 10.13304/j.nykjdb.2015.517

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Gamma aminobutyric acid (GABA) is a non-protein amino acid, synthesized by microorganisms, plants and animals, and widely used as a bioactive component in controlling neurotransmitter signal. Enzyme is only active under acidic environments, which is inconsistent with the condition for bacterium growth. This is not convenient for GABA production by one-step fermentation. According to this problem, this experiment mutated GadB of E. coli AS1.505 through site-directed mutagenesis; constructed 3 expression vectors: pET28a(+)-GadB, pET28a(+)-GadBm1, and pET28a(+)-GadBm2; and studied their status for GABA production. The results showed that after 72 h in neutral buffer solution, DE3（pET28a (+)-GadBm1）and DE3（pET28a (+)-GadBm2）had produced GABA 3.2 and 4.6 times of the control DE3（pET28a (+)-GadB）, respectively. And after 72 h in M9 minimal medium, DE3（pET28a (+)-GadBm1）and DE3（pET28a (+)-GadBm2）had produced GABA 4.32 g/L and 4.65 g/L GABA, respectively, which was 1.17and 1.34 times higher than that of the contrast. The results indicated that site-directed mutated GadB could break the cooperativity in activity change and yield a mutant GadBm2 retained the activity under neutral PH value.

Research on the Soil Restoration Effect of an Acetochlor-degrading Strain Bacillus subtilis L3

YAN Zhi-yu1, ZHAI Bei-bei1, ZHANG Juan2, WANG Shu-xiang1, LI Hong-ya1,

2016, 18(2):  65-71.  DOI: 10.13304/j.nykjdb.2016.007

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The acetochlor-contaminated soil could be repaired by microbial technology, and a high-efficient acetochlor-degrading bacterium named as Bacillus subtilus L3 has been obtained after a series of isolation, selection and identification. In order to further verifiy its actual degradation effects on acetochlor and its restoration effects on contaminated soil, the pot experiment was conducted in this paper. Firstly, this test inspected the effects of degraded bacteria concentration and the degraded time on the degradation of acetochlor in the soil, and secondly, of the application of strain L3 on the amount of indigenous microorganisms, enzyme activity, and nutrient elements in the soil, and on the growth of sensitive crop wheat. The results showed that the final concentration of bacterial liquid which had the highest degradation rate was 5×108 CFU/g soil, and its degradation rate was 92.65% after 50 d; the application of L3 bacteria liquid could recover the edaphon amount to a great degree; invertase activity, urease activity, protease activity and catalase activity increased respectively by 100%, 260%, 56% and 6%; total nitrogen content, rapid available phosphorus content, effective potassium content and the content of organic matter in soil increased respectively by 77%, 159%, 698% and 274%; compared with the control, wheat aerial part heightened 117%, the total fresh weight gained 56%, and root length grew by 27%. The results confirmed a good acetochlor-degrading effect and soil restoration capacity of strain L3, and laid a theoretical foundation for the practical application of strain L3 in soil pollution regulation and restoration.

The Design of Information Management System Based on IOT for Intelligent Greenhouse

ZHAO Wen-bing1,2, MAO Han-ping2*,MA Wan-zheng2,3, HAO Li-ming1

2016, 18(2):  72-77.  DOI: 10.13304/j.nykjdb.2015.385

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According to the needs in intelligent greenhouse information management system to collect and handle large amount and multiple types of data including environment information, crop information, and control information, etc. this paper put forward a scheme of the mixed information management network system for greenhouse, based on IOT, which blend wireless Zigbee network, WIFI high bandwidth wireless network and industry ethernet. Discussion emphases were laid on an information management method by taking SOL SERVER2008 data bank as core, MCGS as collection and demonstration platform, S7-300 as controlling information terminal. After actual verification this scheme could well meet the requirements of an intelligent greenhouse information management.

Influence of Late Frost on Winter Wheat Yield Using MODIS Atmospheric Profile Retrieval Products

YANG Min1, LIU Jun-ming1*, WANG Peng-xin1, HU Xin2, HUANG Jian-xi1, WANG Nian1

2016, 18(2):  78-85.  DOI: 10.13304/j.nykjdb.2015.192

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Late frost, a kind of sudden low temperature agricultural meteorological disasters, occurred with different degrees in nearly years in Huanghuai wheat area of China and had a significant impact on winter wheat yield. According to the severe late frost of winter wheat in Shangqiu in 2013, this paper analyzed the influence of late frost toward winter wheat yield components using MODIS atmospheric profile retrieval products. Firstly, surface temperature (ST) and water vapor of lower atmosphere layer (WVL) series data of the MYD07_L2 products were selected to analyze the process of late frost. Then, some indexes were chosen from temperature and humidity to make a correlation analysis combining with the measured data from frost-damaged wheat. Finally, feature space of temperature and humidity were built using the correlation analysis results to analyze the effect of low temperature and humidity on yield components. The results showed that ST and WVL could reflect the meteorological environment of winter wheat growth. The dates of extreme low temperature matched actual late frost dates well and late frost occurred with the lower temperature and humidity condition. Temperature minimum less than 0℃ had obvious correlation with the grains per spike. The combination of temperature less than 0℃ and humidity obvious overlapped effects on the grains per spike. The results provided a basis for early prediction of impact on winter wheat yields caused by frost after jointing stage.

Preliminary Research on Diagnosis System Design of Wheat Diseases and Pests Based on the Internet of Things

SU Yi-feng§, DU Ke-ming§, LI Ying, SUN Zhong-fu*, ZHENG Fei-xiang

2016, 18(2):  86-94.  DOI: 10.13304/j.nykjdb.2015.459

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Wheat is one of the major grain crops in China, cultivated in large-scale, distributed in vast areas with long growing cycles and multiple varieties. However, it is easily threatened by diseases and pests. Therefore, rapid monitoring and accurate identification of diseases and pests become an important research project. Based on the wheat monitoring system platform previously developed with Internet of Things (IoT), this study designed a remote diagnosis system combining image acquisition with diagnosis methods. The diagnosis methods for 3 common wheat diseases and pests were studied preliminarily, and 4 pictures of wheat leaves contaminated with powdery mildew, rust, aphis and healthy ones were compared and studied by means of image segmentation, feature extraction and digital image classification. The results showed that the recognition rates had reached desired levels. Among them, the recognition rate for powdery mildew was 82.5%, the recognition rates for rust, aphis and healthy leaves were all above 95%. This study combined the image recognition technology with IoT technology. These technology was convenient for tele-transmission of diseases and pests images and multi-peer retrival. These merits have greatly improve our ability in remote identification and diagnosis. This technology has broad development prospect.

A fast 3D Reconstruction for Wheat Plant Architecture Studies in Phenotyping

FANG Wei1, FENG Hui1, YANG Wan-neng1,2, LIU Qian1*

2016, 18(2):  95-101.  DOI: 10.13304/j.nykjdb.2015.503

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The automatic detection technology for plant phenotype plays an important role in agricultural reaserch and crop breeding. However, many 3D features cannot be extracted by 2D technology. Plant architecture (PA), including number of tillers, tiller angle and stem diameter, significantly affects the crop yield for many tillering crops. To acquire these characteristics parameters, traditional method needs huge manual labors, time-consuming, subjective and inaccurate. Therefore, it is impractical to perform manual phenotypic analysis. In order to automate PA parameters collection in crop breeding, a fast 3D reconstruction method was proposed to acquire high throughput PA characteristics parameters. To improve the reconstruction efficiency, parallel computing technique was used on a graphics processing unit (GPU). The processing time was approxemately 10 s of per plant on the Compute Unified Device Architecture (CUDA). This methodology was suitable for a high-throughput phenotyp testing platform.

Application of Edible Casein Films on Food Preservatives

ZHANG Ming-ming1, HU Xian-wang1*, SONG Yong-qiang1, LIANG Ning1,

2016, 18(2):  102-109.  DOI: 10.13304/j.nykjdb.2015.273

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Taking strawberry, potatoes, chicken breast as material, this paper studied the effects of different edible casein membrane treatments on food protein content, soluble sugar content, ascorbic acid content, peroxide value, TBA value and hex anal concentration. The results indicated that in strawberry coating group, the contents of protein, soluble sugar, and ascorbic acid were the highest, followed by fat soluble capsule group. Those contents in water soluble capsule group were slightly higher than not coated. Potato coating film group had lower oxidation degree than the group without coating film. Chicken breast coated or not coated samples provided entirely different senses, antioxidant effect was obvious. Thus, casein coating liquid was superior to the preservation of coated on food, fat soluble capsule was better than water soluble capsule. Coating food preservation technology has better antioxidant ability and antimicrobial effect, to a certain extent, it can prolong food shelf life.

Studies on Degradation Mechanism of Zeaxanthin Processed by Ultrasonic

FENG Meng-ge1, WEI Qi-xin1, SUN Jian-xia2, LU Jun-li1, ZHU Cui-juan1,

2016, 18(2):  110-114.  DOI: 10.13304/j.nykjdb.2015.629

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Focusing on the degradation of zeaxanthin during low temperature ultrasonic extraction, this paper analyzed the mechanism of degradation of zeaxanthinby ultrasonic by testing the UV-visible spectra, concentration and hydroxyl radical intensity of the zeaxanthin samples. The results showed that ultrasonic processing led to the degradation of zeaxanthin samples. Moreover, with the increase of power and processing time, the UV-visible spectra of zeaxanthin changed: the highest absorbance of 453 nm decreased, and so did the zeaxanthin concentration. Based on analyzing the TPA-OH intensity, produced by TPA sample under the same ultrasonic-processing condition as the zeaxanthin, it was found that the hydroxyl radical produced through ultrasonic processing had a positive correlation with the zeaxanthin degradation. This result revealed the rule and mechanism that ultrasonic degraded the zeaxanthin by producing hydroxyl radical.

Effects of Drought Stress on Physiological Property and Growth Parameter of Different Drought Resistance Soybean Cultivars

YANG Liu-qing1§, LIU Hao-ran1§, SUN Guo-wei2, ZHANG Shu-ling1, DONG Li-jun1,

2016, 18(2):  115-120.  DOI: 10.13304/j.nykjdb.2015.392

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In order to clarify the effect of drought stress on physiological characteristics and plant growth parameters of soybean cultivars with different drought resistance, this experiment took drought resistant variety (RD) and drought sensitive variety (SD) as material to analyze the effects of water stress on physiological and biochemical indexes and growth parameters of soybean during development period by pot cultivation method. The results showed that the physiological and biochemical indexes of RD and SD were not significantly different under normal water conditions. While after 15 d with drought stress, the activities of SOD and POD in RD plant leaves were 1.34～1.45 fold and 46～1.69 fold of that in SD, respectively. The hydrogen peroxide (H2O2) and malondialdehyde (MDA) in SD plant leaves were 1.80～2.35 fold and 1.09～1.46 fold of that in RD, respectively. In addition, under drought stress, the root biomass both above and below ground of RD was significantly higher than that of SD, and its root morphology parameters were much better than that of SD. The experimental results showed that for reponsing to drought condition, the drought resistant variety RD adjusted its body physiological changes, and optimized the parameters of root morphology to ensure the normal growth of plant and improve its responsing ability to drought stress.

Grey Correlation Clustering Analysis and Evaluation of Economic Traits of Flue Cured Tobacco Produced in He'nan Province

ZHANG Man-man1, SHAO Hui-fang1*, LIU Guo-qing2, HUANG Wu-xing2,

2016, 18(2):  121-127.  DOI: 10.13304/j.nykjdb.2015.416

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In order to compare the differences in economic characters of flue-cured tobacco between different growing areas in Henan Province, this paper used grey correlation clustering and decision methods to conduct comprehensive evaluation on following 3 targets: tobacco yield, first class, and middle class tobacco leaf proportion in 10 tobacco planting areas of Henan Province. The results indicated that according to the different economic traits of tobacco planted in different areas, the 10 tobacco planting areas could be divided into 4 categories: the first group included Sanmenxia, Luoyang and Jiyuan; the second group included Xinyang; and the third group included Xuchang, Nanyang, Zhumadian, Pingdingshan, Luohe; and the fourth group included Shangqiu tobacco planting areas. The comprehensive performance of tobacco economic traits of various flue-cured groups showed that the performance of Xuchang, Nanyang, Zhumadian, Pingdingshan, Luohe tobacco planting areas was the best, their comprehensive evaluation value was 0.98; followed by Sanmenxia, Luoyang and Jiyuan, their comprehensive evaluation value was 0.93; the performance of Xinyang tobacco planting areas was relatively low and their comprehensive evaluation value was 0.89; Shangqiu tobacco planting areas was the lowest and their comprehensive evaluation value was 0.85.

Enzyme Activities of Saline-alkali Soil and its Relationship with Soil Microbial Biomass and Physicochemical Factor

JING Yu-peng1, LI Yue-jin2*, YAO Yi-ping1, TUO De-bao1, LIN Ya-li2,

2016, 18(2):  128-138.  DOI: 10.13304/j.nykjdb.2015.407

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In order to investigate the soil layer enzyme activity, distribution traits characteristics, and their relation with soil microbial biomass, physicochemical characteristics, this study took 3 soil types with different salinization levels (light, moderate, high) in Tumochuan plain as research objectives to analyze the correlation between soil enzyme activities, microbial biomass and physicochemical characteristics of 0~40 cm layer soils, by canonical correlation and typical correlation, and principal component analysis methods. The results showed that soil invertase, urease and alkaline activities decreased with the increase of soil depth. Their variation rates were 0.65~36.55 mg/g, 0.003~0.018 mg/g and 0.10~0.98 mg/g, respectively. While soil catalase activity increased with the increase of soil depth, and the changing rate was 2.76~3.35 mg/g. Soil invertase, catalase, urease and alkaline phosphatase activities decreased with the increase of total salt content, but soil enzyme activities were significantly different in different salt affected soils and during different seasons. There were obvious canonical correlation between soil enzyme activity and soil physicochemical properties, soil microbe quantity, basically representing general correlation between variables. The correlation between soil enzyme activity and soil physicochemical properties was mainly attributed to pH, organic matter-moisture content, available phosphorus and available potassium of physicochemical factors and invertase, catalase, urease and alkaline phosphatase; while the correlation between soil enzyme activity and soil microbe quantity was to invertase, urease of soil enzyme activities and bacteria, fungi, azotobacter of microbe quantities. The principal component analysis reflected that organic matter, alkaline phosphatase, invertase, actinomyces, cellulose decomposing bacteria and moisture content could be considered as the most important factors affecting soil fertility in Tumochuan plain.

Suitability Assessment of the Land for Freshwater Aquaculture in Tianjin

HU Jing1, SUN Ying-ze1, OUYANG Hai-ying1*, JIA Bao-hong2, LV Xiong-jie2,

2016, 18(2):  139-147.  DOI: 10.13304/j.nykjdb.2015.424

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The evaluation index system of the land for freshwater aquaculture in Tianjin was built based on the analytical hierarchy process (AHP) method with 22 evaluation factors selected from natural resources, conditions for basic development, and prospects of market. In the system, the weights of different layers were determined by Delphi method combined with AHP. Using large quantity of field investigation and collecting relevant statics and maps, a basic map was developed as the evaluation model consists of standardized data. The land used for freshwater aquaculture in Tianjin was divided into 4 grades for different suitabilities: unsuitable, moderately suitable, suitable and very suitable. Results of evaluation indicated that 63 142 hm2 area could be used for freshwater aquaculture in Tianjin. They were mainly moderately suitable and suitable, accounting for 56.56% and 42.38%, respectively. Very suitable area accounted for 0.62%, mainly distributed in southern of Baodi District. Not suitable areas were the least, accounting for 0.44%, mainly distributed in southern of Dongli District. By comparing the present status of aquaculture area with the available areas, the following counties with great potential were found to develop freshwater aquaculture: Ninghe Country, Binhai New Region, Xiqing District and Jinghai Country. Wuqing, Baodi and Beichen Districts were relatively developed, but there was still room for further development. On this basis, the paper analyzed the prospects of aquaculture in various districts and counties were in terms of natural resources and conditions for basic development, and then put forward suggestions for future development. In order to expand the application areas of this suitability evaluation and share the research results, a system were developed for planning and management of aquaculture-the desktop and network version based on ArcGIS. This system has been running on line.

Effect of Ultrasound and Acidification on Morphology and Elution Efficiency of Heavy Metal from Soil Contaminated by Electronic Waste Pickling

LI Ya-pin1,2, YANG Jun1*, LIU Shu-qing2

2016, 18(2):  148-155.  DOI: 10.13304/j.nykjdb.2015.341

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To improve the chemical elution efficiency of heavy metal contaminated sites, this paper took electronic waste pickling site soil of Longtang Town, Qingyuan city, Guangdong Province as research object, and analyzed the effect of ultrasound and acidification elution, etc. parameters on elution efficiency of Cd, Cu, Pb, and Zn from soil contaminated by electronic waste pickling sites through indoor experiment. The results showed that ultrasonic treatment could effectively improve the elution efficiency of soil heavy metals, especially Cu and Zn. Their elution efficiency increased by 9.65% and 15.3%, respectively. After acidification pretreatment, the elution efficiency of Cd, Cu, Pb and Zn increased by 70.2%, 29.0%, 41.1% and 48.54%, respectively. After acidification treatment, the soil pH reduced from 6.89 to 4.00, and the contents of acid extractable of Cd, Cu, Pb and Zn increased by 10.8%、1.26%、11.1%、11.9%. Acidification treatment could turn reducible, which was difficult to be eluted by EDTA into acid extractable and oxidizable that can be eluted easily by EDTA, and thus enhanced the elution efficiency of EDTA on soil heavy metals. Therefore, ultrasound, acidification pretreatment could be used as assistive technology for improving the elution efficiency of soil heavy metals by EDTA.

Research on Countermeasures for Deep-sea Fishery Development in China

YUE Dong-dong, WANG Lu-min*, HUANG Hong-liang, ZHENG Han-feng,

2016, 18(2):  156-164.  DOI: 10.13304/j.nykjdb.2015.524

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Deep-sea fishery is an important industry of strategic significance, an important part of the implementation of agriculture “Going Out” strategy, and an important indicator for the development level of modern fisheries. This paper analyzed the global fisheries resources and fishing area distribution, the international deep-sea fishery management measures, and main features of foreign deep-sea fishery industry. The paper also systematically analyzed the current situation of deep-sea fishery development from 3 aspects, including industrial-scale, structure and support policies, and combed the comprehensive competitiveness of the factors affecting deep-sea fishery from 5 aspects, including fisheries resources, fishing boats and equipment, key technology, overseas comprehensive deep-sea fishery bases, management and market. The paper deemed that the prominent constraints of deep-sea fishery were lacking of coordination between fishing capacity layout and fishing resources, lacking of coordination between dependence on the international consumer market and economic benefits, lacking of coordination between industrial organization degree and deep-sea power goal. Before ending, from the view point of perfecting deep-sea fishery industry system, this paper put forward relative countermeasures and suggestions for realizing the dream of deep-sea fishery power.

Comprehensive Evaluation of Fishery Development Level in China Based on Entropy Weighted TOPSIS Index

LI Bo1,2, CHEN Rui1

2016, 18(2):  165-175.  DOI: 10.13304/j.nykjdb.2015.616

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Fishery is an important part of the national economy in China, and has an important significance to increase farmers′ income. Based on the review of related literature, this paper constructed a comprehensive evaluation index system of fishery development level. Using entropy weighted TOPSIS method and provincial data in 2012, this paper further calculated the index weights and comprehensively evaluated provincial fishery development levels in China. The results showed that there was apparent regional difference in provincial the fishery development levels of China. On one hand, there was polarization patterns in fishery development levels of provincial areas of eastern region and middle region. On the other hand, the fishery development levels of provincial areas of western region and northeastern region were relatively lower than other regions. The related policy implications were discussed based on the results of the analysis.

A Method for Determination of Fluoroquinolone Residues by HPLC with Fluorescence Detector

YANG Yong1,2, LUO Yi3, SU Ju3, WU Lin-lin3, MAO Xiang-jun2, XU Qian-li2*

2016, 18(2):  176-181.  DOI: 10.13304/j.nykjdb.2015.574

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In order to develop a high performance liquid chromatography, high performance liquid chromatography series fluorescence detector (HPLC-FLD) detection method was established, which could detected 6 fluoroquinolones residues in milk at the same time. The sample after acetonitrile containing 5% acetic acid extraction of the vortex, the modified QuEChERS method of HPLC-FLD purification, detection, excitation wavelength 280 nm and emission wavelength of 450 nm, the column was Waters XBridgeTM-C18 column (4.6 mm×250 mm,5 μm), the flow rate of 1 mL/min, the column temperature of 30℃, with acetonitrile and 0.1% formic acid. 20 mmol/L ammonium formate solution as mobile phase gradient elution. In the method of good separation of 6 fluoroquinolones (R>1.5), in 0.01~0.3 g/mL concentration range with good relationship (r=0.999 9), the detection limit is between 0.97~3.91 g/kg, the recovery rate of test in the average recovery rate was 97%~100%. Repeatability, precision, stability test results are good, the relative standard deviation are <3%. The established method is simple, rapid, sensitive and high recovery rate, suitable for mass screening and quantitative determination of 6 kinds of fluoroquinolones residue in milk.

The Determination of Mutton Price Fluctuation Cycle and Policy Implications in China

WANG Ming-li1, LIU Yu-feng1, LV Guan-wang2, SHI Zi-zhong3

2016, 18(2):  182-191.  DOI: 10.13304/j.nykjdb.2015.239

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For a long time, mutton price fluctuates sharply under the effect of frequent external shocks, which is not conducive to the steady development of mutton sheep industry. Therefore, grasping the changes rule of mutton price and calculating the effect of the external impacts are advantageous to the regulation policy-making. The trend cycle of mutton price time series from June 1994 to March 2014 was decomposed based on the B-N decomposition method, the impact of prices fluctuation of other livestock and poultry meat was analyzed based on the VAR model, then the persistent effect of random impact was calculated. The results showed that mutton prices had deterministic trend of steady growth; the actual cyclical fluctuations were very violent, which could be divided into six periods generally. There was a long-term stability between the price of beef and mutton price, however, the pork and chicken price fluctuations had smaller influence on mutton price fluctuations. The cumulative effect of random shocks inhibited the rise of prices generally, but promoted mutton prices into the uplink channel quickly in the past two years.

An Empirical Study on Factors Affecting Herbivorous Animal Husbandry Competitiveness based on Co-integration Analysis ——Taking Gansu Province as a Case

CHEN Hui-ping, ZHANG Yan-rong*, HUANG Bing-kai

2016, 18(2):  192-199.  DOI: 10.13304/j.nykjdb.2015.430

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Herbivorous animal husbandry plays an important role in economic development of Gansu Province. With the support of governments at all levels, herbivorous animal husbandry competitiveness of Gansu Province is constantly enhanced. This paper analyzed the status of herbivorous animal husbandry of Gansu Province by the location quotient analysis method. The results showed that herbivorous animal husbandry competitiveness of Gansu Province was weak, ranking after the 6 major pastoral areas. According to the present development status of herbivorous animal husbandry in Gansu Province, this paper studied the main factors influencing herbivorous animal husbandry competitiveness by co-integration analysis and the index system, including competitive strength, competitive potential and competitive environment. The results showed that the following factors had the most effect on the competitiveness: the original value of fixed assets at each year end of animal husbandry production, R & D personnel, R & D expenditures, internet users of broadband access, milk yield, mountain sheep wool cashmere wool yield, animal husbandry output value, per capita GDP; On the basis of empirical study about the main influencing factors, this paper puts forward several suggestions to strengthen the competitiveness of herbivorous animal husbandry in Gansu Province.

Coupling Degree Analysis of Circular Agriculture in Gansu Province under the Perspective of Ecological Industry Chain and Value Chain

LIANG Fei, DOU Xue-cheng*

2016, 18(2):  200-207.  DOI: 10.13304/j.nykjdb.2015.365

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In order to evaluate the development of circular agriculture in Gansu Province, combining with the relevant concepts of ecological industry chain and value chain, using factor analysis and linear regression method, this paper analyzed the relationship between agricultural economic growth and agricultural environment recycling, and found their coupling trends. The results showed that agricultural resources in Gansu Province were linear trajectory distribution in the period of 1978-2012, agricultural economic growth was the distribution of the two curve trajectory. By coupling degree analysis of the agricultural resource & environment and agricultural economic growth, it could be found that the coupling degree was declining year by year during 1978-2009, which indicated that the influence of agricultural resource & environment on agricultural economic growth is decreasing annually.

Analysis of Development Situation of Crop Transgenic Breeding Research Based on SCI Database

YAN Zhi-hui, ZHENG Huai-guo*, ZHAO Jing-juan, CHUAN Li-min,

2016, 18(2):  208-215.  DOI: 10.13304/j.nykjdb.2015.342

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The transgenic breeding technology is an important way for crop genetic improvement. Through bibliometrical and clustering analysis of subject headings on SCI papers in crop transgenic breeding field, this paper intended to obtain the main research direction, development situation and key technology that restricted its development in transgenic crop breeding field. The results showed that the number of published papers in China took the leading role with 2 956 papers, occupying about 22.1% of the total. The average cited time for papers in Chinese was 7.9. The Chinese Academy of Sciences published the largest number of papers, which research areas covered definition of ideal plants, resistance, cell development, zinc finger proteins implicated in different cellular processes involved in plant development and stress responses, biological metabolism and disease resistance, resistance mechanisms, etc. using model plants. The 3 major crops in transgenic research were rice, corn and wheat. These results provided reference for Chinese researchers to accurately grasp the development trend and key scientific issues in crop transgenic breeding field, and also provided scientific alliance for scientific management organizations in developing supporting policies and determining key projects in crop transgenic breeding area.