Abstract: Superoxide dismutase (SOD) is a critical antioxidant enzyme existing widely in vivo. Taking millet genome database as search platform, this paper carried out genome-wide scanning on superoxide dismutase (SOD) gene family by bioinformatics method and analyzed its bioinformatics. The results indicated that millet SOD gene family included 8 SOD genes unevenly distributed on 6 chromosomes. The exon numbers of these 8 SOD genes were from 1~9, with protein length of amino acid sequence between 56~334 aa. Among 8 SOD proteins, 5 protiens contained Cu-SOD motif, and 3 proteins contained Mn-SOD motif. Cladogram analysis indicated that millet SOD protein sequence had higher homology with that of rice and Sorghum. The results of this study provided certain reference for further understanding SOD gene family and millet antioxidation mechanism.

Key words: Setaria italica, superoxide dismutase, bioinformatics analysis

摘要： 超氧化物歧化酶（superoxide dismutase, SOD）是重要的抗氧化酶，在生物体内广泛存在。以谷子基因数据库为搜索平台，利用生物信息学方法对谷子超氧化物歧化酶基因家族进行全基因组扫描和生物信息学分析。结果表明：谷子SOD基因家族包含8个基因，不均匀的分布在6条染色体上，8个基因的外显子数量为1~9个，蛋白质的氨基酸序列长度在56~334 aa之间。谷子中的8个SOD蛋白，有5个具有Cu-SOD基序，3个具有Mn-SOD基序。进化树分析表明，谷子SOD蛋白与水稻、高粱SOD蛋白序列具有较高的同源性。该研究结果可以为进一步了解谷子SOD基因家族和谷子抗氧化机制提供一定参考。

关键词: 谷子, 超氧化物歧化酶, 生物信息分析