Journal of Agricultural Science and Technology ›› 2019, Vol. 21 ›› Issue (5): 41-48.DOI: 10.13304/j.nykjdb.2018.0312

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Effects of Exogenous Melatonin on Physiological Characteristics of Tobacco Seedlings Under Drought Stress

LI Penghui1§, XIANG Jinyou2§, WANG Lin3, XU Jianqiang4, LI Changjun5, LEI Qiang6, YANG Yide2, ZHANG Xuewei7, LI Huaiqi8, ZHANG Qiming9, JING Yanqiu1*, XIONG Bin3*   

  1. 1.College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002; 2.Yibin Branch of Sichuan Tobacco Company, Sichuan Yibin 644002; 3.Technology Center, Hubei Branch of China Tobacco Industry Co. Ltd., Wuhan 430051; 4.Changde Branch of Hunan Tobacco Company, Hunan Changde 415000; 5.Chongqing Branch of China National Tobacco Company, Chongqing 400023; 6.Sichuan Branch of China National Tobacco Company, Chengdu 610041; 7.Guangdong Branch of China Tobacco Industry Co. Ltd., Guangzhou 510310; 8.Technology Center, Henan Branch of China Tobacco Industry Co. Ltd., Zhengzhou 450016; 9.Tobacco Science Research Institute of Jiangxi Province, Nanchang 330025, China
  • Received:2018-05-21 Online:2019-05-15 Published:2018-07-02

干旱胁迫下外源褪黑素对烟草幼苗生理特性的影响

李鹏辉1§,向金友2§,王林3,徐坚强4,李常军5,雷强6,杨懿德2,张学伟7,李怀奇8,张启明9,景延秋1*,熊斌3*   

  1. 1.河南农业大学烟草学院, 郑州 450002; 2.四川省烟草公司宜宾市公司, 四川 宜宾 644002; 3.湖北中烟工业有限责任公司技术中心, 武汉 430051; 4.湖南省烟草公司常德市公司, 湖南 常德 415000; 5.中国烟草总公司重庆市公司, 重庆 400023; 6.中国烟草总公司四川省公司, 成都 610041; 7.广东中烟工业有限责任公司, 广州 510310; 8.河南中烟工业有限责任公司技术中心, 郑州 450016; 9.江西省烟叶科学研究所, 南昌 330025
  • 通讯作者: *通信作者:景延秋,教授,博士,主要从事烟草化学研究。E-mail:jingyanqiu72t@163.com;熊斌,高级工程师,硕士,主要从事烟草化学研究。E-mail:yinyun12@sina.com
  • 作者简介:§李鹏辉与向金友为共同第一作者。李鹏辉,硕士研究生,研究方向为烟草化学、烟草栽培与烟草加工工艺。E-mail:lipenghuiND@163.com; 向金友,高级农艺师,硕士,主要从事烟草生产技术研发和推广。E-mail:24106446@qq.com。
  • 基金资助:
    江西省烟草公司项目(2016.01.008);四川省烟草公司项目(201851150024089)资助。

Abstract: In order to reveal the physiological effects of exogenous melatonin (MT) on tobacco drought resistance ability, taking Yuyan 10 as experimental material this paper conducted hydroponic experiment and explored the effects of different concentrations MT (50 μmol/L, 100 μmol/L, 200 μmol/L, 500 μmol/L) on tobacco seedlings membranous peroxide, osmotic regulation substances content, antioxidant enzyme activity and photosynthetic characteristics under drought stress. The results showed that drought stress could cause serious damage to physiological function of tobacco seedlings, and the treatments with 50 μmol/L or 500 μmol/L MT had no better mitigating effect on drought stress. While, 100 μmol/L MT could increase the chlorophyⅡ content and photosynthetic rate; enhance activities of superoxide disproportionation enzyme (SOD), peroxidase (POD), catalase (CAT); remove active oxygen free radicals; decrease malondialdehyde (MDA) content; increase accumulation of soluble protein and proline osmotic regulatory substances; improve leaf moisture content and reduce water loss rate, thus effectively alleviated the damage caused by drought stress.

Key words: tobacco seedlings, drought stress, melatonin, physiological characteristics

摘要: 为了揭示外源褪黑素(MT)提高烟草抗旱能力的生理效应,以豫烟10号为供试材料,采用水培实验,探究了干旱胁迫下不同浓度(50 μmol/L、100 μmol/L、200 μmol/L、500 μmol/L)MT对烟草幼苗膜脂过氧化物、渗透调节物质含量、抗氧化酶活性及光合特性的影响。结果表明:干旱胁迫对烟草幼苗的生理功能造成严重损害,50 μmol/L和500 μmol/L MT处理对干旱胁迫的缓解效果不佳,而100 μmol/L MT处理能够提高叶绿素含量和光合速率,增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,清除活性氧自由基,降低丙二醛(MDA)含量,增加可溶性蛋白和脯氨酸渗透调节物质的积累,提高叶片含水率,降低失水率,有效缓解了干旱胁迫的伤害。

关键词: 烟草幼苗, 干旱胁迫, 褪黑素, 生理特性