Abstract: In order to research the effect of the bio-fertilizer suitable on wheat seedling growth, taking Jimai 22 as material, this paper studied the effect of five groups of complex bacteria (two treatment of Bacillus mucilaginosus Krassilnikov + Bacillus subtilis + Bacillus laterosporus, and one treatment of Bacillus laterosporus + Bacillus amyloliquefaciens + Bacillus subtilis, Bacillus subtilis + Bacillus laterosporus +Bacillus mucilaginosus, Bacillus amyloliquefaciens + Bacillus mucilaginosus + Bacillus licheniformis, respectively)  on physiological indexes and soil enzyme activities in wheat seedling stage by dipping seed plate test and pot experiment. The results showed that the effect of Bacillus laterosporus + Bacillus amyloliquefaciens + Bacillus subtilis was better, and the length of bud and main root increased by about 10% compared with the control; the length of root, dry weight of shoot and underground increased continuously at 5 d, 12 d and 19 d after three-leaf stage; the activity of root, and activities of soil urease, neutral phosphatase and catalase increased significantly compared with the control and other treatments. The results of this study provided  scientific basis for further screening of wheat specific bacterial fertilizer.

Key words: bio-fertilizer, physiological index of wheat seedling stage, soil enzyme activity, root vitality

摘要： 为研究适合小麦苗期生长的生物菌肥，以济麦22为试验材料，采用菌液浸种平皿试验和盆栽试验，研究5组复合菌（胶质芽胞杆菌＋枯草芽胞杆菌＋侧胞芽胞杆菌2个处理，侧胞芽胞杆菌+解淀粉芽胞杆菌+枯草芽胞杆菌、枯草芽胞杆菌＋侧胞芽胞杆菌＋胶冻样芽胞杆菌、解淀粉芽胞杆菌＋胶冻样芽胞杆菌＋地衣芽胞杆菌各1个处理）对小麦苗期生理指标和土壤酶活性的影响。结果表明：侧胞芽胞杆菌+解淀粉芽胞杆菌+枯草芽胞杆菌效果较优，与对照相比，小麦芽长和主根长均增加10%左右；三叶期后5 d、12 d、19 d根长、地上部和地下部干重持续增加；根系活力、土壤脲酶、中性磷酸酶和过氧化氢酶活性相比对照极显著提高。该研究结果可为进一步筛选小麦专用菌肥提供科学依据。

关键词: 生物菌肥, 小麦苗期生理指标, 土壤酶活性, 根系活力