Abstract: Salt tolerance in sorghum is significantly differed among various germplasms, development periods and growth conditions, therefore, establishing a comprehensive, objective and accurate method for the evaluation of salt tolerance is of great interest to screen and identify salt resistant sorghum germplasms. To this end, 64 sorghum accessions from a broad genetics background were evaluated for salt tolerance under growth chamber condition. Subsequently, 36 sorghum accessions with different degree of salt resistance were selected for field test of salt tolerance performance at seedling stage. Eventually, 13 accessions were subjected to salt tolerance evaluation back in the growth chamber at seedling stage, again. Both in the field and growth chamber, the seedling height, stem diameter and other morphological indexes along with physiological and biochemical indexes such as superoxide dismutase, proline and malondialdehyde were analyzed by subordinate function method, principal component method and cluster method. So far, two salt-tolerant varieties and two salt-sensitive varieties were stood out for subsequent studies. This study established a comprehensive salt tolerance evaluation method based on multilevel phenotypic analysis, namely, enriched morphological indexes and physiological and biochemical assay indexes were used to systematically and accurately evaluate salt tolerance in sorghum at different development stages (germination and seedling stages) and different growth conditions (field and growth chamber) with various data analysis approaches. The establishment of this method not only provided a valuable reference for the evaluation of salt tolerance in sorghum germplasm resources, but also was beneficial and instructive to the breeding research on salt tolerant sorghum cultivars.

Key words: multilevel phenotypic analysis, salt tolerance, germination, seedling stage, sorghum

摘要： 高粱耐盐性因种质、发育时期及生长条件的不同而存在明显差异，因此建立一套全面、客观、准确的高粱耐盐性鉴定方法，对于高粱耐盐种质的筛选和评价具有重要意义。选择64份高粱材料通过室内萌发期耐盐性评定，从中选择36份不同等级材料进行田间苗期耐盐性鉴定，并从中选出13份材料进行室内苗期耐盐性复筛。通过隶属函数值法、主成分分析法、聚类分析法对田间和室内苗期测定的株高、茎粗等形态指标以及超氧化物歧化酶、脯氨酸、丙二醛等生理生化指标的评价，最终确定耐盐材料2份，盐敏感材料2份。建立了一套基于多重表型的耐盐性综合评价方法，即针对高粱不同生育时期（萌发期、苗期）、不同生长条件（田间与室内）采用不同形态指标与生理生化检测指标，并结合多种数据分析方法对不同高粱材料耐盐性进行准确、系统地评价。该方法的建立对于高粱种质资源的耐盐性评价以及高粱耐盐育种具有一定的参考价值。

关键词: 多重表型分析, 耐盐性, 萌发期, 苗期, 高粱