Abstract: In order to reveal the best water content of soil and harvest period, the differences of water-soluble active components in Salvia miltiorrhiza Bunge under different water contents of soil and during different growth periods were compared. The pot experiments were selected to simulate different water contents of soil. Three soil water treatments, including 75% of field water capacity (θf), 55% of θf and 35% of θf were set up, the growth indexes and four water-soluble active components contents in roots of S. miltiorrhiza were investigated. The results showed that the stem height and leaf length of S. miltiorrhiza increased with the increasing of relative water content in soil. The maximum levels of Danshensu, caffeic acid, rosmarinci acid and salvianolic acid B in roots of S. miltiorrhiza all occurred in late June and another smaller peak of them happened in September. Before September, Danshensu content was extremely significant higher in treatment of 55% θf than that of another two treatments (P<0.01); moreover, salvianolic acid B content of 75% θf treatment was the highest  among three treatments. However after September, the contents of Danshensu and salvianolic acid B of 35% θf treatment was extremely significant higher than those of other treatments (P<0.01). During the whole tested period, caffeic acid content of 55% θf treatment was extremely significant higher than those of other treatments (P<0.01), and rosmarinci acid content of 75% θf treatment was the most highest  among  three treatments. In total, the best harvest period of S. miltiorrhiza roots was in late June. The proper dought of soil was more available for the accumulation of Danshensu and caffeic acid; however, the moist soil  was benefit for the accumulation of rosmarinci acid and salvianolic acid B. The demand for soil water of four water-soluble active components accumulations as sorting: Danshensu< caffeic acid< Salvianolic acid B< rosmarinci acid. The present research provided a base foundation for the normalize cultivation of S. miltiorrhiza in dry areas such as Ningxia Autonomous Region.

Key words: Salvia miltiorrhiza Bunge, water content of soil, water-soluble active components

摘要： 为比较不同土壤水分含量和生长期之间丹参水溶性成分含量的差异，揭示4种丹参有效成分的最佳栽培土壤水分条件和采收期，采用盆栽法控制土壤水分含量，设置土壤水分含量分别为田间最大持水量(θf)的35%、55%、75%，研究不同土壤水分处理对丹参的生长指标和4种水溶性有效成分含量的影响。结果显示，①丹参株高和叶长随土壤水分增加而逐渐升高。②丹参素、咖啡酸、迷迭香酸、丹酚酸B含量均在6月底达到最大值，9月进入第2峰值。③9月之前，丹参素在55% θf下含量极显著高于另外两个处理（P<0.01）；丹酚酸B含量在75% θf下极显著高于另外两个处理（P<0.01）；9月之后则均在35% θf下极显著高于另外两个处理（P<0.01）；整个检测期内，咖啡酸在55% θf、迷迭香酸在75% θf下含量极显著高于另外两个处理（P<0.01）。综上，6月底是获得丹参水溶性成分的最佳采收期，土壤适度干旱利于丹参根部丹参素和咖啡酸合成，土壤湿润利于迷迭香酸和丹酚酸B合成，4种有效成分对水分的需求量表现为：丹参素<咖啡酸<丹酚酸B<迷迭香酸。研究为宁夏等干旱地区丹参的规范化栽培提供一定的理论依据。

关键词: 丹参, 土壤水分, 水溶性有效成分