Abstract: In order to fully utilize sweet tea  resource and screen out active ingredients for hypoglycemic treatment and antioxidance, this paper optimized the HPD-826 resin from four different polarities of macroporous resins by comparing their static adsorption and desorption performances on sweet tea polyphenols; and discussed its conditions of dynamically purified process. Then, the polyphenols after purification were extracted with HPD-826 resin by using organic solvents with different polarities, and the effects of hypoglycemic treatment and antioxidance were determined. The results showed that the optimal conditions of sweet tea polyphenols purified by HPD-826 resin were followed: sample with 2.0 mg·mL-1 mass concentration of solution, 50 mL volume, and 2 BV·h^-1 flow rate, eluent ethanol with 60% volume fraction, 250 mL volume, and 2 BV·h^-1 eluent rate. Under these optimized conditons, the mean purity of polyphenol was up to 81.2%. There were positive correlations between mass concentrations of solvent extracts and inhibition rate of α-glucosidase and clearance rate of DPPH·. The ethyl acetate-soluble fraction from polyphenol purified by HPD-826 resin had the strongest inhibition to α-glucosidase and DPPH·, which showed good value of medicinal development.

Key words: HPD-826 resin, polyphenols, antioxidance, α-glucosidase, inhibitory activity

摘要： 为了从甜茶中得到降血糖和抗自由基氧化的活性成分，通过4种不同极性的大孔树脂对甜茶多酚的静态吸附-解吸性能研究，筛选出HPD-826树脂，探讨其动态纯化甜茶多酚工艺条件；采用不同极性有机溶剂对HPD-826树脂纯化的甜茶多酚进行萃取，测定各相萃取物的降血糖和抗氧化能力。结果表明： HPD-826树脂纯化甜茶多酚的优化工艺条件为上样液(质量浓度2.0 mg·mL^-1、体积50 mL和流速2 BV·h^-1)，洗脱剂乙醇(体积分数60%、体积250 mL和流速 2 BV·h^-1)。在此优化条件下，甜茶多酚平均纯度达到81.2%。甜茶多酚各相萃取物质量浓度与α-葡萄糖苷酶的抑制率和DPPH·的清除率具有正相关性，其中乙酸乙酯相萃取物对α-葡萄糖苷酶和DPPH·都表现出最高的抑制率，具有良好的药用开发价值。

关键词: HPD-826树脂, 多酚, 抗氧化性, α-葡萄糖苷酶, 抑制活性