Abstract: In order to explore the application of HPLC fingerprint in identifying flue-cured tobacco producing areas, this study optimized the extraction condition; constructed HPLC fingerprint on polyphenols of 24 flue-cured tobacco samples from Henan Province; and established geographic identification reflecting polyphenol with tobacco characteristics of Henan. The paper selected 24 samples from Henan, Hunan, Shaanxi and Yunnan 4 provinces and verified the geographic indication established on these 24 samples. The results showed that the geographic indication was high similarity between samples from Henan production areas, while low similarity between other production areas and Henans. Therefore, tobacco from Henan could be identified quickly and accurately. Cluster analysis was conducted on samples from the above 4 areas, and samples from Henan were effectively separated from that produced in other flue-cured tobacco areas. These results were basically the same as that from computer software, indicating the feasibility and accuracy of geographic indication on producing areas by establishing polyphenol HPLC geographic indication for Henan flue-cured tobacco, which was of important guiding significance for objective evaluation of flue-cured tobacco leaf and enterprise procurement.

Key words: flue-cured tobacco, polyphenols, fingerprint, geographic indication, origin discrimination

摘要： 为研究多酚类HPLC指纹图谱在产区识别中的应用，通过提取条件的优化对河南24份烟叶样品的多酚类物质进行HPLC指纹图谱构建，建立了反映河南烟叶特征的多酚类物质地理标识。选取河南、湖南、陕西和云南4个省份得24份样品对建立的地理标识进行验证。结果表明：河南产区样品地理标识相似度较高，而其他产区样品与河南地理标识的相似度较低，从而将河南烟叶准确快速的鉴别出来;将4个产区样品进行聚类分析，河南产区烟叶与其他产区得到有效分离，分析结果与相似度计算软件中的基本一致，表明河南烤烟多酚类HPLC地理标识的建立对样品进行产区判别的可行性和准确性，对烟叶的客观评价和企业采购有重要的指导意义。

关键词: 烤烟, 多酚, 指纹图谱, 地理标识, 产区判别