Table of Content

15 March 2018, Volume 20 Issue 3

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Advantages Analysis of Corn Planting in China

WANG Qiqi1, CHEN Yinjun*

2018, 20(3):  1-9.  DOI: 10.13304/j.nykjdb.2017.0331

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Due to the prominent phenomenon such as high corn price, high corn yield and high corn inventory in recent years, some people think that China has lost advantages in corn production, thus they call on reducing corn planting area sharply and lower corn self-sufficiency rate. However, judging from economic and ecological view, corn production in China still has many advantages. This paper analyzed our advantages in corn by comparing with other crops, and with corn produced in the other countries from the following 5 aspects: planting distribution range, yield potential, cost-benefit, market demand prospect and irrigation requirement. The results showed that corn in China had extensive planting area, strong adaptability to natural conditions and huge yield potential; better planting efficiency than the other arid land crops, bigger reduce space in cost compared to the developed countries; extensive market demand, feed corn would become major requirement in future market; less irrigation water needed and higher utilization efficiency of water resources, helpful for saving regional water resources. In the long run, China should continue to play the corn advantages and not relax corn production.

Effects of Different Rootstocks on Growth and Photosynthetic Characteristics of Large-fruit Castanen henryi ‘Guili 1’

ZHANG Junjie1,2, CHEN Zongyou1*, HAN Yu1,3, WANG Manlian1, TANG Hui1

2018, 20(3):  10-19.  DOI: 10.13304/j.nykjdb.2017.0448

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In order to select excellent rootstock of large-fruit Castanen henryi ‘Guili 1’ and provide theoretical support for scion-stock combination interaction mechanism, C. henryi ‘Guili 1’ was grafted onto C. mollissima and C. Henryi, respectively, and their survival ratios were observed. In addtion, the C. mollissima and C. henryi seedlings were as the controls, and then the growth, Pn-PFD response curve and diurnal variation of photosynthesis of the grafted seedling were measured. The results showed that the survival ratio of the grafted seedling whose rootstock was C. henryias was much higher than that of whose rootstock was C. mollissima. Both kinds of grafted seedlings grew weaker than that of the controls. The differences of light response parameters, such as maximum net photosynthetic rate (Pmax), light saturation point (LSP), light compensation point (LCP), apparent quantum yield (AQY) and dark respiration rate (Rd) on C.henryi ‘Guili 1’ were not significantly affected by 2 different rootstocks. The range of applicable light intensity of 4 kinds of seedlings was C. mollissima＝C. mollissima/Guili 1 (C. henryi ‘Guili 1’ grafted onto C. mollissima)＞C. henryi/Guili 1 (C. henryi ‘Guili 1’ grafted onto C. henryi)＞C. henryi. The diurnal variations of net photosynthetic rate (Pn) in 4 kinds of seedlings were dual-peaked models, and the Pn of 4 kinds of seedlings all peaked at 8:40~10:00 a.m. The midday depression of photosynthesis appeared at 14:00, and the depressive range of C. henryi/Guili 1 and C.henryi were wider than that of C. mollissima and C. mollissima/Guili 1. Compared with C. mollissima and C. mollissima/Guili 1, C. henryi and C. henryi/Guili 1 were more seriously affected by environmental factors, such as scorching heat and intense light. With respect to the trends of daylong changes of photosynthetic parameters, such as Pn, stomatal conductance (Gs), transpiration rate (Tr), internal cellular CO2 concentration (Ci), stomatal limitation (Ls) and water use efficiency (WUE), C. mollissima and C. mollissima/Guili 1, C. henryi/Guili 1 and C. henryi were similar, respectively. The differences of all-day organic matter accumulation on both kinds of grafted seedling were very small. The main physiological and ecological factors affecting the daylong Pn of grafted seedlings were photosynthetically active radiation (PAR), Gs, Tr, Ci and Ls. In terms of affinity, C. henryi was more suitable for the rootstock of C. henryi ‘Guili 1’. But it was recommended that C. mollissima was used as rootstock in high altitude or strong sunshine areas.

Variations of Flavonoids and Soluble Sugars in ‘Northland’ Blueberry Leaf During the Color Changing Process

LI Binbin, HOU Zhixia*, YANG Junfeng, CHEN Lu, WAN Rumeng

2018, 20(3):  20-29.  DOI: 10.13304/j.nykjdb.2017.0210

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Taking 4-year-old ‘Northland’ blueberry as material, this paper studied on the changing rule of soluble sugars and flavonoids during leaf color transformation in autumn and the related relationship, especially the compositions and contents of the 3 kinds flavonoid metabolism pathway and products; expounded the characteristics of soluble sugars and flavonoids in different blueberry leaves during color change procedure in autumn, and analyzed these 2 materials and their relation with temperature. The results showed that the content of proanthocyanidins in green leaves was higher, while the content of anthocyanins in red leaves was relatively higher. With the change of leaf color, the total amount of anthocyanins and main anthocyanin contents were increased successively with larger increasing range in the later stage. While, the total amount of proanthocyanidins was decreased with big changing range at the initial stage. The flavonol content increased continuously but with a smaller range. The change rule of soluble proanthocyanidins was different from that of insoluble proanthocyanidins. The soluble procyanidins increased in middle stage, while the insoluble procyanidins always decreased. The soluble sugar content increased with the change of leaf color, in which sucrose took the highest proportion. The changes of fructose and glucose were inconsistent with those of sucrose, but their total contents were all increased. The relationship between total soluble sugar change and temperature was significantly negative, while that between total fructose, flavonoids and anthocyanin were significantly positive, and that between sucrose and procyanidine was significantly negative. Comprehensive analysis showed that there were differences between contents of flavonoids and soluble sugar in blueberry leaves of different states during color change stage. The content of proanthoyanidins in green leaves was higher, while the contents of soluble sugar and anthocyanin were higher in red leaves. Lower temperature was conducive to the accumulation of soluble sucrose synthesis. The accumulation of flavonoids, especially anthocyanin, was accompanied by the decomposition and consumption of soluble sugars. Above results would be helpful to excavate more function of leaf in health care and R&D of products, and provide a theoretical basis for further studies on the relationship between flavonoids and soluble sugar.

Study on Softening and Metabolism of Starch and Sugar and Related Gene Expression in Postharvest Tobacco Leaves

JIANG Bowen1, WEI Shuo1, WANG Tao2, JIA Hongfang1, GAO Huafeng2,XIE Yan2, HE Fan1, SONG Chaopeng1*

2018, 20(3):  30-38.  DOI: 10.13304/j.nykjdb.2017.0412

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In order to investigate the changing rule of starch and sugar metabolism in harvested tobacco leaves, and its relationship with tobacco softening, flue-cured tobacco variety Yuyan 12 was taken as sample material to test the changes occurred in hardness, respiratory intensity, contents of total sugar, reducing sugar and starch in harvested tobacco leaves; and to analyze the expression levels of genes related to starch and glucose metabolism. The results indicated that the hardness of harvested tobacco leaves declined gradually, showing positive correlation with the changes in starch content (r=0.827*), and negative correlation with the contents of total sugar and reducing sugar （r=-0.964**, -0.958**）. The expression of starch synthesis related genes NtAGPS, NtGBSS, NtSS, NtSBE and NtISO showed a tendency of decreasing. Among them the expression of tobacco amylopectin synthesis related genes NtSS, NtSBE and NtISO in harvested tobacco leaves within 18 h still could maintain a higher level, but 24 h later decreased rapidly. The expression levels of Ntα-amylase, Ntβ-amylase, NtSPS, NtSuS and NtVIN rose first and then decreased. In the harvested tobacco leaves, starch anabolism was weaken, and starch catabolism was strengthened. At the same time, glucose metabolism was gradually exuberant. When the sugar metabolism substrate was depleted or leaf blade aged to a certain extent, the glucose metabolism was weakened again.

Functional Characterization of PatPTS Gene Using Virus Induced Gene Silencing (VIGS) System in Patchouli (Pogostemon cablin)

JIN Qiaochun1, YU Fang2, YU Zongxia1*, FENG Baomin1*

2018, 20(3):  39-45.  DOI: 10.13304/j.nykjdb.2017.0306

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The patchouli oil is mainly stored in the peltate trichome on the aerial part of patchouli. The total RNA was extracted from the leaf and the complete cDNA sequence of patchoulol synthase gene (PatPTS) was cloned via PCR. The virus induced gene silencing(VIGS) system was constructed in patchouli to characterize the function of PatPTS, and the tobacco rattle virus (TRV) was used to silence PatPTS expression. GC-MS and qRT-PCR were used to detect the variations of these sesquiterpenes and PatPTS transcription in leaf after VIGS infection. The contents of β-patchoulene, β-elemene, trans-β-caryophyllene and (-)-patchoulol were decreased significantly after infection for 2 weeks, and the expression level of PatPTS showed similar pattern; which meaned PatPTS was in charge of the biosynthesis of the major components of patchouli oil. The results indicated that the VIGS system in patchouli was successfully established, and silence effects on secondary metabolites production and gene expression were the most effective after infection for 2 weeks, which provided an effective method for the fast screen of gene functions.

Research Progress on Structure Constitution, Biological Function and Separation Methods of Plant Cuticle

GAO Sen, WANG Yahong, SHAO Huifang, XU Zicheng, NIU Lulu,CHEN Zheng, ZHANG Haicong, HUANG Wuxing*

2018, 20(3):  46-54.  DOI: 10.13304/j.nykjdb.2017.0209

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The cuticle membrane consists of cutin and wax. It is a layer of hydrophobic lipid material covering the upper parts of plant, like flower, leaf and non-woody stem organs. It has the functions of limiting water plant excessive loss, resisting ultraviolet radiation, preventing pest invasion, and plays protective roles in the process of plant growth and development, and adaptation to biotic and abiotic stresses. It is very important to study cuticle structure and chemical composition, which are the main reasons influencing its biological function. The paper reviewed the structure composition, biological function and commonly used methods for its separation; discussed problems existing in research, hoping to provide theoretical references for studying its physiological and biological functions in the future.

Retrieving Coastal Soil Saline Based on Landsat Image in Chongming Dongtan

WANG Duoduo1, JIA Wenxiao2, WANG Zhibao1, ZHANG Ruifeng1,CHEN Meitian1, CAI Yongli1*

2018, 20(3):  55-63.  DOI: 10.13304/j.nykjdb.2017.0302

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Currently, the national land resources are facing a serious problem of salt alkali degradation. Taking Chongming Dongtan saline alkali soil in Shanghai as object, this study applied multivariate adaptive regression splines model (MARS) and partial least squares regression (PLSR) to establish regression models of soil salinity and explore spatial pattern of regional saline alkali soil, based on the filed sampling soil saline data, and band reflectance, salinity index(SI), salinity index 1(SI1), normalized difference vegetation index(NDVI), canopy response salinity index (CRSI) and land surface water index(LSWI) calculated from Landsat remote sensing data. The results showed that： ① The coastal soil salinity performed obvious absorption in infrared band, and showed high correlations with infrared band, shortwave infrared band(SWIR1) and NDVI. ② MARS model had better performance in retrieving of soil salinity than PLSR (R2=0.74 and 0.70, respectively). ③ There was high spatial heterogeneity of soil salinity in Chongming Dongtan coastal area, with higher value near water body and intertidal zone, and lower value in forest and farmland. This paper present a fashion for the regional monitoring of soil salinization in coastal area, and provided valuable information for controlling coastal saline alkali soil deterioration and ecological construction of the island.

Simulation and Experimental Research on Rice Seed Metering Device Based on EDEM

XU Hao1, TAO Dongcai1*, TAO Yunhui2, XIAO Bowen1

2018, 20(3):  64-70.  DOI: 10.13304/j.nykjdb.2017.0616

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In order to meet the requirements of planting rice directly in the hill, 4 kinds of different structure groove-wheel seeding device were designed. The EDEM, a discreet element analysis software, was used to simulate and analyze the above 4 types of grooves, and then the tentative was done for the trough planers with different structures. The results showed that when the groove was hookedly-shaped, the angle of the slot was 14.01°and the rotating speed of seed row wheel is 21.09 r/min, with the best rate of average hill diameter being 96.43% and the rate of seed number in the hill being 95.23%, in which the EDEM simulation and test results were basically the same, indicating that the discreet element method for simulation of groove-wheel seeding device was feasible.

Temperature Control Strategy Based on Variable Universe Fuzzy Control Theory for Greenhouse Tomato Cultivation

WANG Jian, XIE Nan

2018, 20(3):  71-79.  DOI: 番茄种植；昼夜温差；温度积分；变论域模糊控制；控制算法

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In order to control accurately the temperature in tomato grown greenhouse in northern China, this study put forward the intelligent temperature control strategy, according to temperature characteristics for each period of tomato growth in greenhouse, using accumulated temperature theory and variable universe fuzzy control theory. Through accumulated temperature method to calculate temperature thresholds, this paper made it adapt to the dynamic changes in external environment, and solved the difficult problem in traditional fuzzy control method by the variable universe fuzzy control theory, due to its structural parameters of relative fixation, yet not applying to high-precision control. Thus, the stability and system response time were greatly enhanced, and the control error was reduced. The simulation results showed that compared to the PID control scheme, this method had a 54.17% and 75% improvement in response time and overshoot aspect. Compared with the traditional fuzzy control scheme, this methods in response time and overshoot aspect had 35.29% and 55.56% improvement. The actual effect of the test showed that compared with the existing control strategies, both in daytime and nighttime, this control strategy could make the average indoor temperature closer to the desired temperature value, and at the same time save nearly 10% energy consumption, thus effectively improved the energy utilization rate.

Development of Apple Intelligent On-line Inspection and Classification System Based on Machine Vision

SHI Ruiyao, TIAN Youwen*, LAI Xingtao, GU Wenjun

2018, 20(3):  80-86.  DOI: 10.13304/j.nykjdb.2017.0580

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At present, the automation grading level of apple was lower. A set of apple intelligent online inspection grading system was designed to achieve an automatic, fast and accurate grading of apple quality. Taking the Hanfu apple as test object, this paper studied on apple grading by machine vision technology. The positive image of apple was divided by threshold segmentation method, and the external contour of apple was extracted by the pixel-by-pixel traversal method. The apple size feature was extracted by calculating the distance from each point to the center of gravity. At the same time, fruit shape features was extracted by calculating the ratio between apple transverse and longitudinal diameters. The support vector machine (SVM) method was used to separate the apples from both sides. Apple color characteristics were extracted by calculating the proportion of red pixels accounted for entire apple pixels, and the defective part was extracted by Fisher statistics. In this study, the hardware of the whole grading system and function of the software was realized. 400 apple samples were graded using this system, and the results showed that the total grading accuracy rate by this system was 95%. The design of this apple intelligent online inspection and grading system based on machine vision overcomed the shortcomings of traditional grading methods, accelerated the automatic degree of quality grading, and was of important significance in the field of studying fruit quality grading.

Application Progress of 3D Printing Technology in Food Fabrication

DU Shanshan1,2, ZHOU Aijun1*, CHEN Hong2*, DONG Xuyan2, WEI Fang2, LV Xin2

2018, 20(3):  87-93.  DOI: 10.13304/j.nykjdb.2017.0214

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As a new type of molding technology, 3D printing is based on the preparation principle of “layered manufacture, layer by layer superposition”. It has the advantages of convenience, flexibility and wide range of application. Food 3D printing technology has given birth to new idea of healthy diet. It can provide unique nutrition meal according to individual requirement. Recently, its application in food processing has been rapidly developed. Especially under the situation of seeking for healthy diets by nowadays society, 3D printing technology has even more shown its superiority in personalized printing. This paper introduced the development status of 3D printing technology; categorized food ingredients available for 3D printing and current printing technologies in food processing; summarized available food printers and their applications in food processing, so as to provide technical guidance for further applications of 3D printing technology in food fabrication.

Research Progress and Production Status of Domestic and Overseas Transgenic Agriculture Products and GM Food Safety

WANG Liping1, WANG Dong2, GONG Yixin3, XU Ying4, SONG Yujing1

2018, 20(3):  94-103.  DOI: 10.13304/j.nykjdb.2017.0515

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Since the GM crops production became commercialized on large scale, enormous economic benefits has been brought to the human society, but there has always been a dispute about the risk and safety of transgenic technology. Therefore, it is necessary to strengthen the studies on the safety evaluation of genetically modified foods (GMF). This paper elaborated the status of GMF safety research at home and abroad, and summarized the cultivation situation, laws and regulations, supervision system, and public attitudes towards GMF in some countries and regions (such as USA, EU, Japan and Brazil). At the same time, combined with our national conditions, and directing at the subject matters of imperfect laws and regulations in transgenic field, lacking of public understanding, and difficulties in supervision of food safety, etc., the paper put forward suggestions as perfecting the relevant laws and regulations; protecting consumers right to learn the truth; establishing reasonable and feasible supervision system from the central to the local governments; strengthening professional knowledge training; constructing a national science popularization platform for genetically modified organism; and strengthening impartial and rational dialogue, etc..

Effects of DPC and DTA-6 on Leaf Senescence Physiological Characteristics, Yield and Quality

DUAN Pengfei1, ZHAO Di2*

2018, 20(3):  104-114.  DOI: 10.13304/j.nykjdb.2017.0215

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In order to discuss the physiological mechanism of postponing cotton leaf senescence after topping, and effects of plant growth regulators on cotton stem leaves senescence, yield and quality, a field experiment was conducted to study the effects of plant growth regulators on cotton leaf senescence physiological characteristics, yield and quality of conventional cotton variety Guoxin 3 and hybrid cotton Guoxin 8 at different growing stages in 2010-2011. Chlorophyll a and total chlorophyll contents in stem leaves from topping to boll opening metaphase of both Guoxin 3 and Guoxin 8 were significantly increased under plant growth regulator treatment. Net photosynthetic rate of both Guoxin 3 and Guoxin 8 was improved from full-bloom to initial boll opening stage, especially at full-bloom stage. The activities of POD from topping to boll opening metaphase of both Guoxin 3 and Guoxin 8 were significantly increased under plant growth regulators (Miantaijin，DPC tablet，DPC powder) treatments. The activities of SOD in Guoxin 3 at the pre-boll opening stage and Guoxin 8 at the post boll opening stage were also improved. However, the activities of CAT were unaffected. Carotenoid contents were significantly increased both in Guoxin 3 and Guoxin 8 at pre-boll opening stage and post boll opening stage, respectively. Peak of oxygen free radicals and MDA contents at initial boll opening stage was significantly reduced in both Guoxin 3 and Guoxin 8. The number of green leaves at boll opening metaphase, and total cotton yield was increased under plant growth regulators treatment. Plant growth regulators treatment also improved upper half staple length, specific breaking strength and uniformity of Guoxin 3 in 2010 and Guoxin 8 in 2010 and 2011. Micronaire value of Guoxin 3 was increased in both 2010 and 2011. Plant growth regulators increased the chlorophyll a and total chlorophyll contents in stem leaves from topping to boll opening metaphase, and increased the photosynthetic rate from full-bloom stage to initial boll opening stage. Plant growth regulators increased number of green leaves at boll opening metaphase and prolonged photosynthetic time, increased the flowering rate of cotton before frost, average boll weight and total cotton yield, and also optimized the cotton quality by certain portion.

Establishment of Fingerprint Geographic Indication of Polyphenols in Flue-cured Tobacco in Henan

ZHENG Yanan, ZHAO Mingqin*

2018, 20(3):  115-123.  DOI: 10.13304/j.nykjdb.2017.0269

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In order to explore the application of HPLC fingerprint in identifying flue-cured tobacco producing areas, this study optimized the extraction condition; constructed HPLC fingerprint on polyphenols of 24 flue-cured tobacco samples from Henan Province; and established geographic identification reflecting polyphenol with tobacco characteristics of Henan. The paper selected 24 samples from Henan, Hunan, Shaanxi and Yunnan 4 provinces and verified the geographic indication established on these 24 samples. The results showed that the geographic indication was high similarity between samples from Henan production areas, while low similarity between other production areas and Henans. Therefore, tobacco from Henan could be identified quickly and accurately. Cluster analysis was conducted on samples from the above 4 areas, and samples from Henan were effectively separated from that produced in other flue-cured tobacco areas. These results were basically the same as that from computer software, indicating the feasibility and accuracy of geographic indication on producing areas by establishing polyphenol HPLC geographic indication for Henan flue-cured tobacco, which was of important guiding significance for objective evaluation of flue-cured tobacco leaf and enterprise procurement.

Study on Transpiration and Water Consumption of Three Typical Afforestation Tree Species in the Soft Sandstone Area of Jungar Banner

LIU Long1, YAO Yunfeng1, GUO Yuefeng1*, QI Wei1,2, WEN Jian1, GAO Yuhan1, YUCHI Wensi1, HAN Zhaomin1

2018, 20(3):  124-131.  DOI: 10.13304/j.nykjdb.2017.0301

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This paper chose 3 typical afforestation species(Caragana korshinskii Kom.,Hippophae rhamnoides Linn., Pinus tabuliformis) in the soft sandstone area of Ordos, Inner Mongolia as study objects and compared their transpiration rates by SF60 stem flow meter. The results showed that: ①The transpiration rate of these 3 tree species showed a obvious changing rule in daytime and nighttime. The daytime transpiration rate was much higher than the nighttime when there was a weak and stable stem flow indicating a remarkable law with single and double peaks. ②The flow rate curve of the 3 tree species in the sunny days was relatively gentle and the peak range was wider. The flow started earlier and stopped later; in the rainy days, the peak value was steeper with narrow range, and the flow started later, stopped earlier; in the cloudy days, the flow rate curve was in the middle of the sunny days and rainy days. ③The transpiration rate of the 3 tree species was significantly correlated with the soil water content, and the correlation coefficients were all over 0.985. These results aimed at providing theoretical basis for forest planting management and rational utilization of water resources in arid areas.

Research Progress on the Effect of Physical and Chemical Properties of Biomass Activated Carbon on the Adsorption Properties of VOCs

ZHAN Shijie1,2, MENG Haibo1*, CHENG Hongsheng1, SHEN Yujun1

2018, 20(3):  132-138.  DOI: 10.13304/j.nykjdb.2017.0267

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Volatile organic compounds (VOCs) are important air pollutants. Adsorption is an effective means to control the environmental pollution by volatile organic compounds. Studying the effect of adsorbent properties on adsorption performance is of important significance for the development of adsorption technology. This paper reviewed the present situation at home and abroad about the effect of physical and chemical properties of bioactive carbon on the adsorption performance of VOCs. The results showed that the specific surface area, pore volume and structure are the main factors affecting the adsorption performance of activated carbon. However, there were few studies on the adsorption performance and influencing factors of bioactive charcoal in the presence of various components VOCs. In future, the related research in this field should be strengthened.

Preparation and Comparative Analysis of Florfenicol Solid Dispersions Using Different Polymers for Pharmaceutical

LI Shengli1,2, LIU Baoguang1, ZHAO Xiaoning1, YUAN Li1, LIU Jianhua1,PAN Yushan1, WU Hua1*

2018, 20(3):  139-144.  DOI: 10.13304/j.nykjdb.2017.0232

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Preparation of solid dispersion by polymers for pharmaceuticals is one of the most effective and convenient methods to improve the solubility and bio-availability of many hydrophobic drugs. In order to investigate the differences of several carriers commonly used in clinic practice, this study took polyethyleneglycol (PEG)6000, and PEG4000, polyvinyl pyrrolidone (PVP) k30, PVPk15 and poloxamer 188 as carriers and prepared florfenicol solid dispersions (SDs) by melting method and solvent-melting method with 5 different polymers for pharmace-uticals, and validated the SDs by dissolution rate method. Solubility and cumulative dissolution rate of florfenicol(FF), physical mixture and SDs were measured and analyzed comparatively. The results showed that the solubility of SDs was raised compared with FF itself. Meanwhile, the efficiency of solubilization was PEG6000>PEG4000>PVPk30>PVPk15>188. Especially, PEG6000 polymers could enhance the solubility remarkably. The best matching was FF:PEG6000 (1∶4). Conclusion, these methods for SDs preparation were simple, convenient, and the quality of SDs can be controlled easily. Among them, especially PEG6000 has the best solubilization effect and can be widely applied.

Evaluation Method for Crop Breeding Material Based on Improvement of Gray Correlation Degree and TOPSIS

PAN Shouhui1,2, WANG Kaiyi1,2*, WANG Zhibin1,2, LIU Zhongqiang1,ZHAO Xiangyu1, LIU Chao1

2018, 20(3):  145-154.  DOI: 10.13304/j.nykjdb.2017.0613

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The traditional empirical method for crop breeding material evaluation is difficult to meet the requirements of large-scale, programmed and data based commercial breeding, and breeders need urgently a scientific, fast and efficient method to evaluate crop breeding material. This paper proposed a method for evaluating crop breeding material based on improved gray correlation degree and TOPSIS, which could quickly evaluate breeding material according to the comprehensive performance of its traits. The proposed method consisted of the following steps: First, a comprehensive evaluation index system of breeding material was constructed by gray correlation analysis of crop traits. Then, the weight of each trait index was obtained by combining the information entropy method and expert scoring method. Finally, the comprehensive score and ranking of each material was obtained based on the TOPSIS. The trait data of 2015 National Rice Variety Regional Test Huanghuai Japonica Rice Group A was chosen to verify the effectiveness of our proposed method. The results showed that the proposed method could effectively identify the excellent varieties with good comprehensive performance from all tested varieties and was superior to the entropy method. The proposed method had been integrated into large-scale commercial breeding software “Golden Seed Breeding Platform (GSBP)”, which had been successfully implemented in 2 seed leading enterprises of China.