Effects of Sodium Dinitrate with Bacillus Sbutilis Complex on Growth and Physiological Indexes of Tobacco Seedlings

doi:10.13304/j.nykjdb.2021.0128

Abstract

Abstract:

In order to study the effects of different concentrations combination of plant growth regulators and microorganism agents on quality of tobacco seedlings， new flue-cured tobacco strain 20619 with floating seedlings as the experimental material， different concentrations mixture of sodium nitrophenolate & Bacillus subtilis agent was sprayed on 4 leaves 1 heart of tobacco seedlings， and determined and analyzed main growth and physiological indexes of tobacco seedlings with 8 leaves & 1 heart. The result showed that in 8 leaves 1 heart of tobacco seedlings after sprayed compound of sodium nitrophenolate & Bacillus subtilis agent， its dry matter accumulation content， root activity， chlorophyll content and nitrate reductase activity were better than that of the control， and dry weight （4.98 g·plant^-1）， ratio of dry to fresh weight， roots activity （93.01 μg·g^-1·h^-1）， chlorophyll content （0.548 5 mg·g^-1） & nitrate reductase （1.434 U·g^-1） of tobacco seedlings were the highest of the treatment of spraied compounding agents of sodium nitrophenolate 20 mg & Bacillus subtilis agent 7.5 g， and activities of catalase， superoxide dismutase， peroxidase and malondialdehyde in leaves were the highest. It showed that spraying the mixture of sodium nitrophenolate & Bacillus subtilis agent on tobacco seedlings is beneficial to improve the quality of tobacco seedlings and cultivating strong seedlings.

Key words: flue-cured tobacco, growth index, physiological index, sodium nitrophenolate, Bacillus subtilis

摘要：

为探讨不同浓度的植物生长调节剂和微生物菌剂复配施用对烟苗素质的影响，以烤烟新品系20619为材料，采用漂浮育苗，在烟苗4叶1心期喷施不同浓度的复硝酚钠和枯草芽孢杆菌菌剂复配剂，对8叶1心（成苗期）烟苗主要生长和生理指标进行测定与分析。结果表明，复硝酚钠和枯草芽孢杆菌菌剂复配喷施后，8叶1心烟苗的干物质积累、根系活力、叶片叶绿素含量及硝酸还原酶活性，均好于对照；且以喷施20 mg的1.8%复硝酚钠和7.5 g的枯草芽孢杆菌菌剂处理，烟苗全株干重（4.98 g·株^-1）、干鲜比、根系活力（93.01 μg·g^-1·h^-1）、叶片叶绿素含量（0.548 5 mg·g^-1）及硝酸还原酶（1.434 U·g^-1）活性均表现最高；叶片中的过氧化氢酶、超氧化物岐化酶和过氧化物酶活性以及丙二醛含量均表现为最高。表明对4叶1心烟苗喷施复硝酚钠和枯草芽孢杆菌菌剂复配剂，有利于提高烟苗素质和培育壮苗。

关键词: 烤烟, 生长指标, 生理指标, 复硝酚钠, 枯草芽孢杆菌

CLC Number:

S572

Tianwei PENG, Huiya XIE, Sijun LI, Yixuan LIU, Kaifeng SHUAI, Yuanyuan PENG, Qing WANG, Diqin LI. Effects of Sodium Dinitrate with Bacillus Sbutilis Complex on Growth and Physiological Indexes of Tobacco Seedlings[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 154-161.

彭田伟, 谢会雅, 李思军, 刘怡轩, 帅开峰, 彭媛媛, 王青, 李迪秦. 复硝酚钠和枯草芽孢杆菌复配对烟苗生长和生理指标的影响[J]. 中国农业科技导报, 2022, 24(4): 154-161.

Figures/Tables 8

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处理 Treatments	复硝酚钠 Sodium dinitrophenol		枯草芽孢杆菌菌剂 Bacillus subtilis agent
处理 Treatments	施用量 Applied content/mg	含量 Content/%	施用量 Applied content/g	含量 Content/%
T1	20.0	0.398	7.5	0.148
T2	20.0	0.398	0.0	0.000
T3	15.0	0.299	7.5	0.148
T4	15.0	0.299	0.0	0.000
T5	10.0	0.199	7.5	0.148
T6	10.0	0.199	0.0	0.000
CK	0.0	0.000	0.0	0.000

处理 Treatments	复硝酚钠 Sodium dinitrophenol		枯草芽孢杆菌菌剂 Bacillus subtilis agent
处理 Treatments	施用量 Applied content/mg	含量 Content/%	施用量 Applied content/g	含量 Content/%
T1	20.0	0.398	7.5	0.148
T2	20.0	0.398	0.0	0.000
T3	15.0	0.299	7.5	0.148
T4	15.0	0.299	0.0	0.000
T5	10.0	0.199	7.5	0.148
T6	10.0	0.199	0.0	0.000
CK	0.0	0.000	0.0	0.000

处理 Treatment	根系活力 Roots vigor/（µg·g^-1·h^-1）
T1	93.01±0.24 a
T2	72.07±0.92 d
T3	84.99±1.78 b
T4	71.66±0.43 d
T5	78.56±0.66 c
T6	68.37±0.39 e
CK	63.87±9.64 f

处理 Treatment	根系活力 Roots vigor/（µg·g^-1·h^-1）
T1	93.01±0.24 a
T2	72.07±0.92 d
T3	84.99±1.78 b
T4	71.66±0.43 d
T5	78.56±0.66 c
T6	68.37±0.39 e
CK	63.87±9.64 f

处理 Treatment	叶绿素a含量 Chlorophyll a content/ （mg·g^-1）	叶绿素b含量 Chlorophyll b content/ （mg·g^-1）	叶绿素（a+b）含量 Chlorophyll （a+b） content/ （mg·g^-1）
T1	0.368 0±0.002 9 a	0.180 5±0.003 9 a	0.548 4±0.003 4 a
T2	0.312 8±0.002 6 c	0.126 7±0.001 4 c	0.439 5±0.004 0 c
T3	0.348 6±0.003 9 b	0.146 1±0.001 7 b	0.494 7±0.005 1 b
T4	0.297 6±0.000 9 d	0.116 6±0.002 6 d	0.414 2±0.003 5 d
T5	0.333 3±0.005 1 c	0.125 1±0.002 9 c	0.458 4±0.005 4 c
T6	0.271 6±0.001 7 e	0.089 1±0.002 2 e	0.360 7±0.002 9 e
CK	0.222 7±0.023 1 f	0.073 7±0.001 0 f	0.296 4±0.022 7 f