Abstract:

To understand the fungal composition of the stem base susceptible parts of adzio bean and mung bean， the fungal community in basal susceptible parts of adzabella and mung bean stems were sequenced by Illumina high-throughput sequencing. The results showed that a total of 231 operational taxonomic units （OTUs） were obtained from 6 samples of adzio bean and mung bean， and the common OTUs were 97. In fungal compositions of the stem base susceptible parts of adzio bean and mung bean， Ascomycota was the common dominant phylum， Sordariomycetes was the common dominant class， Hypocreales was the common dominant order， Nectriaceae was the common dominant family. And Fusarium was the common dominant genus， and the relative abundances in adzio bean and mung bean were 32.94% and 18.38%， respectively. The results of Alpha diversity showed that the abundance of fungal community in susceptible stems of mung bean was higher than that of adzola bean， and the diversities of fungal community in adzio bean and mung bean were similar， which provided a theoretical basis for further study the pathogenic mechanism of fungal communities in the stem susceptible parts of adzio bean and mung bean.

Key words: adzuki bean, mung bean, Illumina high-throughput sequencing, dominant fungi

摘要：

为了解小豆和绿豆茎基感病部位的真菌组成，采用Illumina高通量测序对小豆和绿豆茎基感病部位的真菌群落结构进行分析。结果表明，小豆和绿豆6个茎基感病部位真菌群落样品测序优化后共得到231个OTU （operational taxonomic units），共同OTU为97个。2种作物茎基感病部位真菌群落中共同的优势菌门为子囊菌门（Ascomycota），优势菌纲为粪壳菌纲（Sordariomycetes），优势菌目为肉座菌目（Hypocreales），优势菌科为从赤壳科（Nectriaceae）。对优势菌属分析发现，小豆和绿豆茎基感病部位镰刀菌属（Fusarium）处于绝对优势，相对丰度分别为32.94%和18.38%。Alpha多样性指数结果表明，绿豆茎基感病部位的真菌群落丰度高于小豆茎基感病部位的真菌群落丰度，但二者真菌群落多样性相似，为进一步研究小豆和绿豆茎基感病部位真菌群落的致病机理提供了理论依据。

关键词: 小豆, 绿豆, Illumina高通量测序, 优势菌