Growth and Physiological Response of 3 Different Provenances of Pueraria lobate Under Low Phosphorus Stress

doi:10.13304/j.nykjdb.2021.0400

Abstract

Abstract:

In order to reveal the adaptive mechanism of Pueraria lobate seedlings to low phosphorus stress， taking 3 different provenances of P.lobata from Australia， and Hunan and Jiangsu in China as experimental materials， and 3 different KH₂PO₄ treatments were set at a concentration of 0.5 （normal phosphorus）， 0.05 （low phosphorus） and 0.005 （very low phosphorus） mmol·L^-1. The growth and physiological response of P.lobata seedlings from 3 different provenances under different phosphorus treatments were measured. The results showed that the root indexes such as root length， root surface area and root diameter of three different provenances of P. lobata increased significantly under 0.05 mmol·L^-1 treatment， leaf area， leaf perimeter and other leaf indexes also showed an upward trend （P<0.05）， P. lobata had certain resistance to low phosphorus. Under 0.005 mmol·L^-1 phosphorus treatment， the root morphological indexes of P. lobata from Hunan and Jiangsu were obviously suppressed， but the leaf morphological indexes of P. lobata from Hunan showed an increasing trend. The contents of proline， soluble sugar， malondialdehyde and activities of antioxidant enzymes （SOD， POD， CAT） in roots and leaves of P. lobata from 3 different provenances increased significantly under low and very low phosphorus treatments （P<0.05）， but the content of soluble protein decreased. In summary， the P. lobata from Australian had strong tolerance of low phosphorus and had a high value for planting and promotion.

Key words: Pueraria lobate, low phosphorus stress, morphological characteristics, physiological response

摘要：

为揭示葛藤（Pueraria lobate）幼苗对低磷胁迫的适应机制，选取来自澳大利亚和我国湖南和江苏的3个不同种源的葛藤为试验材料，设置0.5（常磷）、0.05（低磷）和0.005 （极低磷） mmol·L^-13种不同KH₂PO₄浓度处理，测定不同磷处理下3个不同种源葛藤幼苗的生长及生理响应。结果表明，0.05 mmol·L^-1磷处理下，3个不同种源葛藤的根长、根表面积、根直径等根系指标和叶面积、叶周长等叶片指标均呈显著上升趋势（P<0.05），表明葛藤具有一定的耐低磷性；在0.005 mmol·L^-1磷处理下，湖南和江苏葛藤的根系指标较澳大利亚葛藤受到了明显抑制，但湖南葛藤的叶片形态指标呈增长趋势。3个不同种源葛藤在低磷胁迫下的根和叶中脯氨酸、可溶性糖、丙二醛含量及抗氧化酶活性均显著增加（P<0.05），但可溶性蛋白含量下降。综上所述，澳大利亚葛藤的耐低磷能力强，具有较高的种植推广价值。

关键词: 葛藤, 低磷胁迫, 形态特征, 生理响应

CLC Number:

S548

Shuwei ZHANG, Lili ZHAO, Chao CHEN, Jingchen TAN, Li ZHANG, Yi XI. Growth and Physiological Response of 3 Different Provenances of Pueraria lobate Under Low Phosphorus Stress[J]. Journal of Agricultural Science and Technology, 2022, 24(1): 71-82.

张淑炜, 赵丽丽, 陈超, 谭景晨, 张莉, 席溢. 低磷胁迫下3种不同种源葛藤的生长生理响应[J]. 中国农业科技导报, 2022, 24(1): 71-82.

Figures/Tables 8

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大量元素Macronutrient		微量元素Micronutrient
名称Name	浓度Concentration/（mmol·L^-1）	名称Name	浓度Concentration/（μmol·L^-1）
KNO₃	1.0	H₃BO₃	1.00
Ca（NO₃）₂	2.0	MnSO₄	1.00
CaCl₂	1.5	CuSO₄	0.50
MgSO₄	0.5	ZnSO₄	1.00
KCl	1.8	（NH₄）₆Mo₇O₂₄	0.10
		Na₂-EDTA	50.00
		FeSO₄	0.05

大量元素Macronutrient		微量元素Micronutrient
名称Name	浓度Concentration/（mmol·L^-1）	名称Name	浓度Concentration/（μmol·L^-1）
KNO₃	1.0	H₃BO₃	1.00
Ca（NO₃）₂	2.0	MnSO₄	1.00
CaCl₂	1.5	CuSO₄	0.50
MgSO₄	0.5	ZnSO₄	1.00
KCl	1.8	（NH₄）₆Mo₇O₂₄	0.10
		Na₂-EDTA	50.00
		FeSO₄	0.05

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter /mm	地上部鲜重 Shoot fresh weight/g	地下部鲜重 Root fresh weight/g	地上部干重Shoot dry weight/g	地下部干重Root dry weight/g	根冠比 Root-shoot ratio
P_0.5A	15.66±4.36 αa	2.19±0.31 βb	1.57±0.18 αβb	0.57±0.06 βa	0.26±0.03 αβa	0.05±0.01 αβa	0.19±0.06 αa
P_0.05A	12.60±3.07 αa	2.56±0.30 αa	1.88±0.25 βa	0.59±0.10 βa	0.31±0.06 βa	0.05±0.01 αβa	0.16±0.06 αa
P_0.005A	13.37±3.58 αa	2.12±0.28 βab	1.52±0.33 αab	0.52±0.12 αa	0.23±0.04 αa	0.05±0.02 αa	0.21±0.08 αa
P_0.5H	12.56±5.13 αb	2.79±0.33 αa	1.16±0.40 βb	0.52±0.08 βa	0.19±0.02 βb	0.04±0.01 βa	0.21±0.08 αa
P_0.05H	14.57±4.41 αab	2.69±0.33 αa	1.86±0.26 βa	0.55±0.07 βa	0.37±0.05 αβa	0.05±0.02 βa	0.13±0.04 αa
P_0.005H	16.55±3.96 αa	2.75±0.25 αa	1.51±0.16 αab	0.37±0.08 βb	0.20±0.05 αb	0.03±0.08 αa	0.15±0.01 αa
P_0.5J	15.67±4.24 αa	2.65±0.54 αa	2.01±0.53 αb	0.88±0.11 αa	0.33±0.13 αb	0.08±0.04 αa	0.25±0.14 αa
P_0.05J	13.97±3.47 αa	2.69±0.58 αa	2.67±0.37 αa	0.90±0.12 αa	0.46±0.04 αa	0.08±0.03 αa	0.16±0.08 αa
P_0.005J	14.88±3.73 αa	2.38±0.45 βa	1.41±0.23 αc	0.54±0.06 αb	0.22±0.06 αc	0.05±0.07 αa	0.26±0.08 αa

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter /mm	地上部鲜重 Shoot fresh weight/g	地下部鲜重 Root fresh weight/g	地上部干重Shoot dry weight/g	地下部干重Root dry weight/g	根冠比 Root-shoot ratio
P_0.5A	15.66±4.36 αa	2.19±0.31 βb	1.57±0.18 αβb	0.57±0.06 βa	0.26±0.03 αβa	0.05±0.01 αβa	0.19±0.06 αa
P_0.05A	12.60±3.07 αa	2.56±0.30 αa	1.88±0.25 βa	0.59±0.10 βa	0.31±0.06 βa	0.05±0.01 αβa	0.16±0.06 αa
P_0.005A	13.37±3.58 αa	2.12±0.28 βab	1.52±0.33 αab	0.52±0.12 αa	0.23±0.04 αa	0.05±0.02 αa	0.21±0.08 αa
P_0.5H	12.56±5.13 αb	2.79±0.33 αa	1.16±0.40 βb	0.52±0.08 βa	0.19±0.02 βb	0.04±0.01 βa	0.21±0.08 αa
P_0.05H	14.57±4.41 αab	2.69±0.33 αa	1.86±0.26 βa	0.55±0.07 βa	0.37±0.05 αβa	0.05±0.02 βa	0.13±0.04 αa
P_0.005H	16.55±3.96 αa	2.75±0.25 αa	1.51±0.16 αab	0.37±0.08 βb	0.20±0.05 αb	0.03±0.08 αa	0.15±0.01 αa
P_0.5J	15.67±4.24 αa	2.65±0.54 αa	2.01±0.53 αb	0.88±0.11 αa	0.33±0.13 αb	0.08±0.04 αa	0.25±0.14 αa
P_0.05J	13.97±3.47 αa	2.69±0.58 αa	2.67±0.37 αa	0.90±0.12 αa	0.46±0.04 αa	0.08±0.03 αa	0.16±0.08 αa
P_0.005J	14.88±3.73 αa	2.38±0.45 βa	1.41±0.23 αc	0.54±0.06 αb	0.22±0.06 αc	0.05±0.07 αa	0.26±0.08 αa

处理Treatment	总根长 Total root length /cm	根表面积 Root surface area/cm²	根直径 Root average diameter/mm	根体积 Root volume/cm³	根尖数 Root tips No.	根毛数 Root hairs No.
P_0.5A	101.97±20.20 αb	21.67±7.04 αa	0.69±0.06 αa	0.37±0.10 αβa	462.75±35.21 βb	659.00±114.81 βa
P_0.05A	180.50±21.49 αa	26.48±4.74 αa	0.72±0.11 βb	0.48±0.08 αa	691.50±32.84 αa	695.00±107.33 αa
P_0.005A	101.34±7.04 αb	18.87±7.62 αa	0.65±0.22 αab	0.35±0.12 αa	406.00±51.46 αc	399.50±86.30 βb
P_0.5H	129.70±7.63 αa	22.09±10.06 αa	0.58±0.10 αa	0.31±0.10 βa	670.25±20.85 αa	362.75±46.67 γa
P_0.05H	134.98±15.03 βa	23.26±4.36 αa	0.55±0.06 βa	0.32±0.09 βa	513.00±77.73 βab	464.00±69.07 βa
P_0.005H	95.21±10.22 αb	16.70±3.52 αa	0.56±0.08 αa	0.24±0.08 αa	391.25±84.78 αb	380.00±56.22 βa
P_0.5J	118.68±27.92 αa	26.41±3.64 αa	0.73±0.14 αa	0.48±0.09 αa	344.50±105.69 γa	884.00±62.92 αa
P_0.05J	126.13±37.49 βa	26.60±4.29 αa	0.76±0.14 βa	0.48±0.07 αa	348.50±75.59 γa	911.25±99.72 αa
P_0.005J	93.46±16.93 αa	19.14±4.92 αa	0.67±0.09 αa	0.32±0.08 αb	329.50±32.75 αa	599.00±76.00 αb