Abstract:

In order to define the relationship between gonad traits and molecular markers and to improve gonad traits in urchin breeding, correlation analysis of urchin growth characteristics and gonads color were carried out by microsatellite marker. The genetic structure of urchin (Strongylocentrotus intermedius) F1 population is analyzed using 28 polymorphic microsatellite markers. The results indicated that 2~5 alleles were detected for each locus, with  total 80 alleles for 28 loci. The average effective alleles number (Ne) and mean effective alleles were 2.857 1 and 0827 0, respectively; the observed heterozygosity (No) average value and expected heterozygosity average value(He) were 0.515 0 and 0.530 4, respectively. The average polymorphism information content (PIC) was 0.402 6, and the probability value of chi-square test showed that 9 loci had significantly deviated from Hardy-Weinberg equilibrium (P<0.01). The general linear model (GLM) was used to analyze the linkage and found that TS131, TS38 and TS105 were correlated with test height, body weight and volume, respectively; and 4 markers (TS38,TS85,TS105 and TS108) and one (TS41) were significantly correlated (P<0.05) and very significantly correlated with urchin gonad traits L* (Dark-Light); 2 markers (TS101 and TS41) were significantly and very significantly correlated with urchin gonad traits a*(Green-Red); 3 markers (TS41, TS114 and TS128) were significantly correlated with urchin gonad traits b* (Blue-Yellow); 2 markers (TS114 and TS46) were significantly and very significantly correlated with Aristotles lantern weight. The above results could provide reference data for further studies on Strongylocentrotus intermediuss QTL locations and theoretical guidance for Strongylocentrotus intermediuss cultivation and breeding.

Key words: Strongylocentrotus intermedius, genetic structure, correlation analysis, microsatellite

摘要：

为了明确性腺性状和分子标记之间的关系,在海胆选育工作中提高性腺性状,利用微卫星标记对海胆各生长性状及性腺颜色进行相关分析。选用28个虾夷马粪海胆多态性微卫星分子标记对虾夷马粪海胆自交F1代家系群体的进行了检测分析,结果表明:共检测到80个等位基因,平均等位基因数为2.857 1个;平均有效等位基因数为0.827 0个; 观测杂合度平均值0.515 0,期望杂合度平均值为0.530 4;平均多态信息含量（PIC）为0.402 6,卡方检验显示其中有9个位点发生了偏离(P<0.01)。采用一般线性模型(GLM)进行连锁显著性检验,发现与壳高、体重和体积显著相关（P<0.05）的微卫星标记各1个,分别为TS131、TS38和TS105；与海胆颜色L*值（暗-亮）显著和极显著相关的微卫星标记分别为4个 (TS38、TS85、TS105、TS108)和1个(TS41)；与海胆颜色a*值（绿-红）显著和极显著相关的微卫星标记各1个(TS101 TS41)；与海胆颜色b*值（蓝-黄）显著相关的微卫星标记3个(TS41、TS114和TS128)；与海胆口器重显著和极显著相关的微卫星标记各1个(TS114和TS46)。该研究结果可以为虾夷马粪海胆的相关性状进一步QTL定位提供参考数据,并为虾夷马粪海胆的养殖和选育提供理论指导。

关键词: 虾夷马粪海胆;遗传结构;相关性分析;微卫星