Studies on Identifying Pi-ta2 Rice Blast Resistant Genes in Yunnan Local Rice Varieties by Using SSR DNA Marker

doi:10.3969/j.issn.1008-0864.2012.01.06

›› 2012, Vol. 14 ›› Issue (1): 43-48.DOI: 10.3969/j.issn.1008-0864.2012.01.06

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Studies on Identifying Pi-ta2 Rice Blast Resistant Genes in Yunnan Local Rice Varieties by Using SSR DNA Marker

LI Jin-bin1, LI Ding2, SUN Yi-ding2, ZHANG Qing1, XU Ming-hui2

(1.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205|2.Key Laboratory of Biotechnology Research of Yunnan Province, Key Laboratory of Southwest Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Institute of Biotechnology and Germplasm Resources,
Yunnan Academy of Agricultural Sciences, Kunming 650223, China)

Received:2011-08-29 Revised:2011-10-11 Online:2012-02-15 Published:2012-02-13

利用SSR分子标记检测云南地方稻种抗稻瘟病基因Pi-ta2的研究

李进斌1,李鼎2,孙一丁2,张庆1,许明辉2

(1.云南省农业科学院农业环境资源研究所, 昆明 650205|2.云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业部西南作物基因资源与种质创制重点实验室, 昆明 650223)

通讯作者: 许明辉,研究员,博士,主要从事水稻生物技术育种。E-mail:xuminhui@sohu.com
作者简介:李进斌,副研究员,博士,主要从事植物抗病机理研究。E-mail:kmlijinbin@yahoo.com。
基金资助:
云南省自然科学基金(2010ZC173);国家自然科学基金(31160355);云南省作物资源生物技术创新研究团队项目资助。

Abstract

Abstract:

Vertical resistant gene Pi-ta2 plays an important role in breeding blast resistant rice in Yunnan Province. In this study, the resistant gene Pi-ta2 in 220 rice landraces from Yunnan Province was effectively determined by using SSR marker OSR32. 15 accessions were selected for identification by artificial inoculation. The results showed that Pi-ta2 was found in 25 accessions from 123 indica (20.3%) and 51 accessions from 97 japonica (52.58%), which indicated that Pi-ta2 frequency in japonica is higher than indica. The cultivars with Pi-ta2 gene were distributed in 29 counties out of 5 rice producing regions, mainly in japonica rice producing area at southern region and north-eastern plateau in Yunnan province. The results indicated that the pathogenic assays with rice blast fungus were consistent with the detection by molecular marker OSR32. This study preliminary definitudes the distribution of resistance gene Pi-ta2 in Yunnan landrace rice resources. This will be of guiding significance for exploring blast resistant genes and distributing rice varieties.

Key words: Yunnan, rice landrace, rice blast, Pi-ta2 gene, DNA marker

摘要：

垂直抗性基因Pi-ta2在云南抗稻瘟病育种中具有重要的利用价值。利用与抗稻瘟病基因Pi-ta2连锁的SSR引物OSR32对云南地方稻种二级核心种质中的220份抗性基因Pi-ta2进行了检测,并选择15份材料进行了人工接种鉴定。分子标记检测表明,123份籼稻有25份含有Pi-ta2基因,占20.3%；97份粳稻有51份含有该基因,占52.58%,粳稻比籼稻高；携带Pi-ta2基因的材料分布在云南5个稻区中的29个县,较为广泛,南部边缘和滇东北高原粳稻区分布点较多。结果表明,人工接种鉴定与分子鉴定结果完全相符,验证了SSR引物OSR32鉴定抗稻瘟病基因Pi-ta2的可行性。初步明确了Pi-ta2基因在云南地方稻种资源中的分布情况,对水稻抗性基因发掘和品种布局具有指导作用。

关键词: 云南;地方稻种;稻瘟病;Pi-ta2基因;分子标记

CLC Number:

S511.034

LI Jin-bin1, LI Ding2, SUN Yi-ding2, ZHANG Qing1, XU Ming-hui2. Studies on Identifying Pi-ta2 Rice Blast Resistant Genes in Yunnan Local Rice Varieties by Using SSR DNA Marker[J]. , 2012, 14(1): 43-48.

李进斌1,李鼎2,孙一丁2,张庆1,许明辉2. 利用SSR分子标记检测云南地方稻种抗稻瘟病基因Pi-ta2的研究[J]. , 2012, 14(1): 43-48.

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Studies on Identifying Pi-ta2 Rice Blast Resistant Genes in Yunnan Local Rice Varieties by Using SSR DNA Marker

利用SSR分子标记检测云南地方稻种抗稻瘟病基因Pi-ta2的研究

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