›› 2012, Vol. 14 ›› Issue (1): 76-84.DOI: 10.3969/j.issn.1008-0864.2012.01.11
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LIU Xiao-qing, CHEN Ru-mei
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柳小庆,陈茹梅
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作者简介:
基金资助:
转基因生物新品种培育重大专项(2008ZX08003-002)资助。
Abstract:
In transgenic research and production practice, researchers often expect target gene can efficiently express in transgenic organisms, so as to achieve the objective-obtaining crops highly resistant to insecticide and herbicide, or using plants as bioreactor to produce vaccines, antibiotics and other medical products and industrial proteins, including enzymes with high added-value etc. In this paper, by a lot of research literatures, we reviewed various strategies, which could enhance the efficiency of foreign gene expression. From the integration of the foreign gene, the regulation of foreign gene in transcription level and the protein separation after the foreign gene expression selecting 3 levels to illustrate the advantages and disadvantages of relevant strategies, so as to offer reference for the construction of expressive vectors in transgenic research.
Key words: foreign gene, gene expression efficiency, transgenic plant
摘要:
在转基因研究和生产应用中,人们往往期待目的基因能在转基因生物中高效表达从而实现相关目的——获得具有对杀虫剂、除草剂高抗性的农作物,或以植物作为生物反应器生产具有高附加值的疫苗、抗生素等医药用品以及工业用蛋白、酶类等。综述了各种用于增强外源基因表达效率的策略,从外源基因的整合、转录水平的调节以及外源基因翻译后的蛋白分选三个水平阐述提高外源基因表达的相关策略的利弊,从而为转基因研究提表达供载体构建方面的参考依据。
关键词: 外源基因;基因表达效率;转基因植物
CLC Number:
Q786
LIU Xiao-qing, CHEN Ru-mei. Strategies for Enhancing Expression of Foreign Genes in Transgenic Plant[J]. , 2012, 14(1): 76-84.
柳小庆,陈茹梅. 增强外源基因在转基因植物中表达的策略[J]. , 2012, 14(1): 76-84.
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URL: https://www.nkdb.net/EN/10.3969/j.issn.1008-0864.2012.01.11
https://www.nkdb.net/EN/Y2012/V14/I1/76