›› 2012, Vol. 14 ›› Issue (2): 56-61.DOI: 10.3969/j.issn.1008-0864.2012.02.09.

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Segregation Distortion Analysis of Molecular Markers in RIL Population of Brassica napus

DING Guang-da1,2, YANG Mei1,2, LI Xing-mei3, SHI Lei1,2, XU Fang-sen1,2   

  1. (1.Microelement Research Centre, Huazhong Agriculture University, Wuhan 430070;2.National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070|3.Hankou College, Wuhan 430212, China)
  • Received:2011-03-02 Revised:2011-10-11 Online:2012-04-15 Published:2012-02-24

甘蓝型油菜分子标记在重组自交系群体中的偏分离分析

丁广大1,2*,杨美1,2*,李兴美3,石磊1,2,徐芳森1,2   

  1. (1.华中农业大学微量元素研究中心, 武汉 430070;2.华中农业大学作物遗传改良国家重点实验室, 武汉 430070|3.汉口学院, 武汉 430212)
  • 通讯作者: 徐芳森,教授,博士生导师,研究方向为植物营养遗传。
  • 作者简介:*丁广大与杨美为共同第一作者。丁广大,讲师,博士,主要从事植物营养遗传研究。E-mail:dgd@mail.hzau.edu.cn。杨美,助理研究员,博士,主要从事植物营养遗传研究。E-mail:yangmei815815@webmail.hzau.edu.cn。
  • 基金资助:

    国家973计划项目(2011CB100301);国家863计划项目(2006AA10A112)资助。

Abstract:

A genetic linkage map comprising 733 molecular markers of Brassica napus was constructed using F10 population consisting of 124 recombinant inbred lines derived from a cross between P-inefficient cv. B104-2 and P-efficient cv. Eyouchangjia. Segregation distortion analysis was performed among the 733 polymorphic markers. The result indicated that 218 markers (29.7%) showed significant segregation distortion(P<0.05), favoring either the marker alleles of female parent B104-2 (136,62.4%) or male parent Eyouchangjia (82,37.6%). Totally, 24 segregation distortion regions (SDRs) were detected among 13 different chromosomes. Based on this result, the phenomenon and causes for the segregation distortion were analyzed and discussed in this paper.

Key words: Brassica napus, recombined inbred line, molecular markers, segregation distortion

摘要:

以甘蓝型油菜磷高效品种鄂油长荚和磷低效品种B104-2为亲本杂交得到的F10群体作为构图群体,共124个单株,构建了一张含733个分子标记的甘蓝型油菜分子遗传图谱。在这些分子标记中,通过偏分离分析发现,有218个分子标记发生奇异分离(P<0.05),占总连图标记数的29.7%。其中,偏向母本B104-2有136个,占62.4%,偏向父本鄂油长荚有82个,占37.6%。同时,在13个连锁群上发现了24个偏分离热点区域。对这种偏分离现象和原因进行了分析讨论。

关键词: 甘蓝型油菜;重组自交系;分子标记;偏分离

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