Abstract:

As a new biodegradable polymer materials, Poly-β-hydroxybutyrate (PHB) has attracted wide attention from people day by day. This study adopts heavy-ion mutation breeding technology, using 12C6+ ion beams to mutate the PHB producing strain-Bacillus Z-3, then screen a PHB high yielding strain A11 from a mount of mutant strains. Its PHB output can reach 1.404 g/L. This is 1.33 times as compared with the original strain. The results indicate that heavy-ion mutation breeding technology has good mutagenic effects. It is an effective method and way to microbial mutation breeding.

Key words: poly-β-hydroxybutyrate, heavy ion, mutagenesis, selective breeding, high-yield strain

摘要：

聚β-羟基丁酸酯(PHB)作为一种新型的生物可降解高分子材料,日益受到人们的广泛重视。利用12C6+离子束对一株聚β-羟基丁酸酯（PHB）产生菌——芽孢杆菌Z-3进行诱变,然后进行筛选,从大量突变株中最终选育出一株PHB高产菌株A11,其PHB产量达到1.404 g/L,是出发菌株的1.33倍。研究结果揭示,重离子诱变育种技术具有良好的诱变效果,是一种有效的微生物诱变育种方法和途径。

关键词: 聚β-羟基丁酸;重离子;诱变;选育;高产菌株