Abstract:

By the feature of easy acquisition, low cost and short cycle, plasmid reference materials could be used for GMO quantifications. In this experiment, real-time quantitative PCR was used to quantify GMO rapeseed TOPAS 19/2 collaboratively with 7 laboratories. The genome substitutability, collaborative experiment and uncertainty evaluation were carried out on transgenic rapeseed TOPAS19/2 plasmid molecules. T test showed that there were no significant differences between the slope of standard curve and linear correlation coefficient produced by endogenous and exogenous genes of two standards. The statistical analysis conducted on several laboratories′ fixed data showed that the quantity value of Rapeseed TOPAS 19/2 was 0.910 with an uncertainty of 0.013 (K=2).

Key words: genetically modified TOPAS19/2 plasmid, substitutability, Real-time quantitative polymerase chain reaction (PCR), uncertainty

摘要：

质粒分子是转基因产品核酸定量检测的一类新型标准物质,具有易制备、周期短、成本低等特点。采用实时荧光定量PCR技术,并协同7家实验室对转基因油菜TOPAS 19/2质粒分子进行了基因组的可替代性研究、协同实验研究及不确定度评定。T检验表明,质粒和基因组所产生的内源和外源基因标准曲线的斜率和线性相关系数没有显著性差异。对多家定值的数据进行了统计分析得出,TOPAS19/2质粒分子的量值结果0.910,扩展标准不确定度（K=2）为0.013。

关键词: 转基因油菜TOPAS 19/2质粒分子;可替代性;实时荧光定量PCR;不确定度