Loading...

Table of Content

    11 May 2012, Volume 14 Issue 3
    Analysis of Development Directions in Basic Research Related to Bio-industry from the Perspective of NSFC
    YANG Xin-quan1, LV Shu-mei1, CHEN Zhao-bo2, BIAN Xiu-xiu3, LIN Min4
    1, 1(1):  1-10.  DOI: 10.3969/j.issn.1008-0864.2012.03.01
    Asbtract ( )   PDF (1327KB) ( )  
    Related Articles | Metrics

    Bio-industry is currently an important strategic emerging industry. It takes an important position in promoting comprehensive national strength of China. This paper analyzed the development status of basic research in Chinas bio-industry from the supporting scope, quantity, amount of funds and achievement outputs, and pointed out directions for developing basic research in bio-industry; and suggestions for promoting the development of basic research in bio-industry.

    Analysis of China’s Investment for Agricultural Technology
    YUAN Xue-guo1, ZHENG Ji-ye2, LI Jing-suo3
    1, 1(1):  11-15.  DOI: 10.3969/j.issn.1008-0864.2012.03.02
    Asbtract ( )   PDF (527KB) ( )  
    Related Articles | Metrics

    The ultimate way out for Chinas agriculture lies in technology innovation and progress. The level of investment for agricultural science and technology is an important index for judging Chinas agricultural science and technology state. To realize the linkage between investment for agricultural science and technology and agriculture development is a prerequisite for strengthening agricultural scientific and technological innovation and progress. Based on the twice national statistics of research and development (R & D) resources inventory data and existing research literature about agricultural technology investment, this paper analyzes the quantity and structure of Chinas investment for agricultural science and technology and compares it with agricultural R & D funds invested by various countries in the world.  The paper also analyzes the problem existing in the structure of Chinas agricultural investment for science and technology, and puts up policy and suggestion from the view of optimizing resources of agricultural science and technology.

    Enlightenment of European Beekeeping Development Status to China
    CHEN Li-hong1,2, ZHANG Fu-xing1, WU Jie1, Siriwat Wongsiri2, Romanee Sanguandeek
    1, 1(1):  16-21.  DOI: 10.3969/j.issn.1008-0864.2012.03.03
    Asbtract ( )   PDF (1870KB) ( )  
    Related Articles | Metrics

    China, with approximately 8,200,000 honeybee hives and 300,000 ton honey production in 2009, is the largest beekeeping country in the world. However, Chinas apiculture still lag far behind the developed countries in the model of beekeeping production, bee-products quality and safety, pollination industrialization, etc. This paper presents a brief introduction about the current status of apiculture in Europe. It also expounds and summarizes developed European countries experiences. Combining the reality of beekeeping in China, we found the gap between China and the developed European countries. The paper also puts out the existing and eager to be solved problems, so as to promote the sustainable development of apiculture in China.

    Research Progress on Signaling Pathway and Adaptive |Response under Phosphorus Deficiency Stress in Plants
    YU Li-juan, ZENG Ke-wen, LUI Li, FAN Xing-ming
    1, 1(1):  22-30.  DOI: 10.3969/j.issn.1008-0864.2012.03.04
    Asbtract ( )   PDF (1186KB) ( )  
    Related Articles | Metrics

    Phosphorus is one of essential macronutrients required for plant growth and development, but the soluble inorganic phosphate is deficient in 40%~60% of the worlds arable lands. Therefore the plant growth and crop yield are limited. During the long evolution process, plants have developed many strategies to response to Pi deficiency under the synergies between ‘early’ genes and ‘late’ genes. The ‘early’ genes respond rapidly and are involved in sensing Pi deficiency and transducing information and the ‘late’ genes regarding the morphological, physiological or metabolic alternations can improve the Pi acquisition, transportation and cyclic utilization efficiency. Aiming at further understanding how plants response and adapt to Pi deficiency and develop new crops with high tolerance to Pi deficiency, this paper expounds the research progress on plant tolerance to phosphorus deficiency from early signaling events, and late morphological, physiological and metabolism adaptations mechanism, so as to provide scientific basis for developing crop varieties tolerant to Pi deficiency.

    Cooperative Experiment and Uncertainty Analysis for Transgenic Rapeseed TOPAS 19/2 Plasmid Molecule
    LIU Fang-fang1, ZHANG Ling2, SONG Gui-wen3, CAO Ying-long4, SHENG Ling-hui2, HUA
    1, 1(1):  31-35.  DOI: 10.3969/j.issn.1008-0864.2012.03.05
    Asbtract ( )   PDF (1128KB) ( )  
    Related Articles | Metrics

    By the feature of easy acquisition, low cost and short cycle, plasmid reference materials could be used for GMO quantifications. In this experiment, real-time quantitative PCR was used to quantify GMO rapeseed TOPAS 19/2 collaboratively with 7 laboratories. The genome substitutability, collaborative experiment and uncertainty evaluation were carried out on transgenic rapeseed TOPAS19/2 plasmid molecules. T test showed that there were no significant differences between the slope of standard curve and linear correlation coefficient produced by endogenous and exogenous genes of two standards. The statistical analysis conducted on several laboratories′ fixed data showed that the quantity value of Rapeseed TOPAS 19/2 was 0.910 with an uncertainty of 0.013 (K=2).

    Optimization of Conditions for Callus Induction and Initial Analysis of Influencing Factors for Genetic Transformation in Foxtail Millet
    WANG Han-yu1*, DU Yan-wei2*, ZHAO Jin-feng2, ZHANG Xi-wen2, YU Ai-li1,2
    1, 1(1):  36-42.  DOI: 10.3969/j.issn.1008-0864.2012.03.06
    Asbtract ( )   PDF (2066KB) ( )  
    Related Articles | Metrics

    At present, studies on foxtail millet tissue culture and genetic transformation are relatively less, the callus induction of different explants and its transformation efficiency is relatively low. This paper analyzes the formative factors of callus induction and optimizes the inducing conditions by using different foxtail millet shoot apex as explants. The results showed that the optimized embryonic callus induction rate could be as high as 75.4% and the callus differentiation rate  was about 15%. In addition, we transformed the callus using agro-bacterium tumefaciens-mediated, and conducted GUS staining analysis. The result indicated that the transformation of callus GUS transient expression efficiency could be up to 22.3%. In this study, shoot tip explants could reduce the restriction of seasons and regions. The preliminary results showed that the optimized callus induction rate was greatly increased and the GUS transformation efficiency was also improved. This result has provided reference data for future studied on millet tissue culture and genetic transformation.

    Growth and Mutton Performance Analysis of Crossbred Lambs in Alpine Pastoral Area of Qilian Mountains
    WANG Xi-nian1, WANG Ji-qing1, ZHOU Zhi-de2, BAI Tian-lin2, ZHANG Hai-xia2,
    1, 1(1):  43-48.  DOI: 10.3969/j.issn.1008-0864.2012.03.07.
    Asbtract ( )   PDF (1141KB) ( )  
    Related Articles | Metrics

    In order to optimize the hybrid pattern of lamb production in alpine pastoral area of Qilian mountains, a 3 years field experiment was conducted to observe 4 cross combination (Texel、White Suffolk、Bond and Australia Merino were used as male parent,and Gansu Alpine Merino was used as female) by the method of artificial insemination from 2008 to 2010. The results showed that the weaning weight of Texel F1 was 27.71 kg, being significantly higher than the other combinations. The 6-month-old weight of Texel F1 was 31.04 kg, which was higher than Bond、White Suffolk、Australian Merino and the control group for 8.79%, 14.15%, 18.42% and 33.97%, respectively. The carcass weight, dressing percentage, net meat percentage, bone weight and loin muscle area of Texel F1 were the highest, but the ratio of bone to meat was the least. Meanwhile, the pH value and tenderness were better than the others. Therefore, the Texel was the best male,and the group of the Texel X Gansu Alpine Merino was the ideal cross combination for lamb production in alpine pastoral area of Qilian mountains.

    A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests
    GE Dong-hua, SU Jun-ping, SONG Ping, NANGONG Zi-yan, WANG Qin-ying
    1, 1(1):  49-55.  DOI: 10.3969/j.issn.1008-0864.2012.03.08
    Asbtract ( )   PDF (1602KB) ( )  
    Related Articles | Metrics

    Bacillus thuringiensis (Bt) strain MB-15, isolated from the soil in Hebei Province, was high toxic against Lepidoptera vegetable pests. Compared with Bt HD-1, the toxicities of Bt MB-15 against Helicoverpa armigera, Spodoptera exigua, Plutella xylostella, Spodoptera litura and Pieris rapae all were higher than that of Bt HD-1 by bioassay in lab. The supernatant of Bt MB-15 fermentation could remarkably enhance the insecticidal activity of Bt MB-15 crystal protein. The bipyramidal crystals were observed by microscopic observation in Bt MB-15 strain. SDS-PAGE analysis revealed that Bt MB-15 produced crystal proteins of 130.0 kDa and 65.0 kDa. Bt MB-15 contained cry1Ac, cry2Aa, cry1I and vip3Aa gene by PCR-RFLP analysis. The results showed that Bt MB-15 could have the potential and commercial development value against Lepidoptera vegetable pests.

    Intein-based Biosensor
    ZHAO Zhong-lin1, MA Xin2, LI Yan3, LI Shu-ying4, YUAN Chao1
    1, 1(1):  56-60.  DOI: 10.3969/j.issn.1008-0864.2012.03.09.
    Asbtract ( )   PDF (1399KB) ( )  
    Related Articles | Metrics

    The development of biosensors for the analysis of complex biological problems is essential both for the study of human diseases and validation of potential drug molecules. A novel application of intein-mediated protein splicing technologies has led to the development of a biosensor. Inteins have played critical roles in design of many assays and biosensors enabling efficient and precise analysis of important biological processes, such as enzyme activity, hormone receptor, vitamins and protein-protein interactions. In this paper, we review the application and development of intein-based biosensors.

    Design and Realization of Reconstructing LAI Time-series Data by Particle Filter
    LI Man-man, LIU Jun-ming, WANG Peng-xin
    1, 1(1):  61-68.  DOI: 10.3969/j.issn.1008-0864.2012.03.10
    Asbtract ( )   PDF (1862KB) ( )  
    Related Articles | Metrics

    Leaf area index (LAI) time-series data retrieved from remote sensing images have been widely used in monitoring crop growth. However, affected by atmosphere and other conditions, the data might be underestimated and even missing. Therefore, an algorithm was designed in this paper to reconstruct the remote sensing LAI time-series data by particle filter. Particle filter was used for the reconstruction. LAI was used as a variable to localize WOFOST model. Reconstruction of LAI time-series data can be done through assimilation of the remote sensing LAI data and WOFOST-LAI data. The algorithm simplified WOFOST model to be the nonlinear evolution of LAI changing with time, which was used as the state transition equation of re-sampling particle filter. And it got the observation equation with measured LAI data and the remote sensing LAI time-series data, to establish assimilation model of LAI time-series data. And the weighted particles represented the posterior distribution of LAI, then re-sampled the particles in the iteration in order to reconstruct LAI time-series data at the point and in the region. We reconstructed the LAI time-series data of Hebei Province in 2010 with this algorithm. According to the results, we could get better LAI time-series data at the point and in the region with this particle filter-based algorithm, which was closer to the actual growth conditions of crop. And it could also make up for the loss of remote sensing LAI time-series data. Therefore, the reconstructed LAI data could be very supportive for monitoring crop growth.

    Construction and Application of JavaEE-Asp.net——based Information Database System of Honeybee Resources in China
    LIU Zhi-guang, LV Li-ping, DING Gui-ling, GUO Hai-kun, QIN Yue, SHI Wei
    1, 1(1):  69-73.  DOI: 10.3969/j.issn.1008-0864.2012.03.11
    Asbtract ( )   PDF (2263KB) ( )  
    Related Articles | Metrics

    The WEB-based honeybee resources information database system in China was constructed by JavaEE-Asp.net technology. Honeybee resources information data, natural resources of nectar plants data, natural conditions parameters were collected from the workstation of China Bee Research System. The response-ask-response is the major operating model of this database system. Interface friendly, correct data numerical statement and easy to maintain are its characteristics. Users can browse and inquiry data information by visiting Http://www.brpdb.com.

    Research Progress in Capillary Electrophoresis and Micro-fluidic Chip Electrophoresis Applied in Testing Genetically Modified Food
    LI Yong-xin, LI Yuan-qian, He Ling, Li Can
    1, 1(1):  74-79.  DOI: 10.3969/j.issn.1008-0864.2012.03.12
    Asbtract ( )   PDF (1048KB) ( )  
    Related Articles | Metrics

    In the past few years, genetically modified organs as food (GMC) have entered into peoples daily life. The fast, sensitive and reliable testing methods have played a decisive role in protecting the customers right to know and to choose, safeguarding the smooth implementation of labeling system for genetically modified food (GMF). Capillary electrophoresis and micro-fluidic chip electrophoresis, as relatively new technology has made up the deficiencies of traditional gel electrophoresis with their high flux, high sensitivity, fast and low consumption. This paper summarized and assessed the common methods for testing GMF and laid emphasis in reviewing the research progress made in capillary and micro-fluidic chip electrophoresis for testing GMF in recent years.

    Development and Utilization of Banana Bud Edible  and Medical Values
    LIU Sheng-cai, LAI Zhong-xiong
    1, 1(1):  80-84.  DOI: 10.3969/j.issn.1008-0864.2012.03.13
    Asbtract ( )   PDF (1004KB) ( )  
    Related Articles | Metrics

    Banana bud is rich in contents of mineral element, amino acid, protein, unsaturated fatty acid, dietary fiber, etc, and is of important edible value. Meanwhile, it can cure or prevent diabetes and heart cerebrovascular disease, diminish inflammation, stop itches, promote the wound healing, and have antioxidant effect. So it is of important significance to fully develop and utilize the edible and medical values of banana bud and to explore a new special and healthy vegetable variety, so as to improve the additional economic benefit of banana planting in China, to prolong its industry chain, to enhance competitive strength of banana industry, and to open up a new way for farmers to increase their incomes.

    Optimization of Ethanol Extraction Technology from Propolis by Response Surface Methodology
    ZHAO Ya-zhou, TIAN Wen-li, GUO Zhan-bao, GAO Ling-yu, PENG Wen-jun
    1, 1(1):  85-93.  DOI: 10.3969/j.issn.1008-0864.2012.03.14
    Asbtract ( )   PDF (2168KB) ( )  
    Related Articles | Metrics

    Propolis has always been utilized as functional food for its superior health effect. In order to improve the quality and production efficiency of propolis products, on the basis of fully simulating the actual production conditions, we analyzed the influencing factors (extracting time, ethanol concentration, solid-liquid ratio, distilling frequency and distilled temperature) to extraction ratio and general flavonoids content of propolis by response surface methodology (RSM). The results showed that extraction ratio and general flavoniods content predicted by regression model for the propolis samples were 38.324 6% and 199.776 mg/g, respectively in this study. The optimal ethanolic extraction conditions of propolis were as fallowing: extracting time was 24 h, ethanol concentration was 95%, solid-liquid ratio was 10∶1, extracting frequency was twice, and extracting temperature was room temperature. In order to reach the objective of guiding the production, this experiment conclusion should be inspected by the actual production workshop.

    Studies on Analysis of Trace Elements in Hulless Barley by ICP-OES
    QIU Cheng, YU Yao-bin, WU Xue-lian
    1, 1(1):  94-100.  DOI: 10.3969/j.issn.1008-0864.2012.03.15
    Asbtract ( )   PDF (1213KB) ( )  
    Related Articles | Metrics

    Hulless barley was pretreated by wet digestion method (HNO3 and HCIO4), the trace elements in hulless barley: iron, magnesium, calcium, manganese, and the pollution index of heavy metals: lead, cadmium, copper and zinc were determined by ICP-OES. The results indicated that the recovery rate of the method were between 91.0% and 106.3%, with relative standard deviations of less than 3.3%. ICP-OES is sensitive, simple, rapid and accurate with pretreating and determining. This method has been applied to simultaneous determination of multi-trace elements in samples. The results will be satisfactory. The experiment data could provide an accurate and credible evidence for further exploitation of hulless barley.

    Effects of Nitrogen Treatment on Rice Yield and Leaves Conformation Characteristics of ‘Xindao No.18’
    YIN Chun-yuan, WANG Shu-yu, XUE Ying-zheng, LIU He-mei, ZHANG Xu,
    1, 1(1):  101-106.  DOI: 10.3969/j.issn.1008-0864.2012.03.16
    Asbtract ( )   PDF (1140KB) ( )  
    Related Articles | Metrics

    In order to explicit the optimal N fertilizer application in ‘Xindao No.18’ and the relationship between rice yield and leaves characteristics, a field experiment with 0, 232.5 kg/hm2, 255.0 kg/hm2, 277.5 kg/hm2, 300 kg/hm2, 322.5 kg/hm2 and 345 kg/hm2 N fertilizer application was carried out in 2011 at the farm of Xinxiang Academy of Agricultural Sciences Henan Province. The difference and the relationship between rice yield and characteristics of the top 3 leaves were investigated using ‘Xindao No.18’ under different N levels. The results showed that rice yield rose firstly and then fell down as N fertilizer application increasing. When the yield reached maximal value at 255 kg/hm2 N level, the comprehensive expression of the top 3 leaves was better than those of other N levels. The infection of N fertilizer on the length of top 2nd leaf was much bigger than that of the top 1st leaf, while the width of top 1st leaf than that of top 3rd leaf. This showed that the effects of N fertilizer on the leaf traits mainly showed on the length of top 2nd leaf and the width of top 1st leaf. The multi-dimensional gradually regression analysis indicated that the established regression equation took yield as the dependent variable, and the leaf conformation traits as the independent variables respectively by using the length of top 2nd leaf, the width of top 1st leaf and LAI reached the  highly significant level (r=0.996 2**). A conclusion indicated that the length of top 2nd leaf, the width of top 1st leaf and LAI contributed more to yield.

    Effect of Nitrogen Application Level on Head Milled Rice in Association with Source-bank Structure in Mid-Season Hybrid Rice
    XU Fu-xian, XIONG Hong, ZHU Yong-chuan, ZHANG Lin, GUO Xiao-yi, LIU Mao
    1, 1(1):  107-114.  DOI: 10.3969/j.issn.1008-0864.2012.03.17
    Asbtract ( )   PDF (2055KB) ( )  
    Related Articles | Metrics

    In this paper, the relationship between the effects of nitrogen application level on head milled rice and source-sink structure at full panicle stage was investigated with 26 mid-season hybrid rice combinations. The results indicated there was a highly significant negative correlation between the head milled rice under 2 amounts of nitrogen application and in number of spikelets per panicle. The reason was that under low nitrogen application level, leaf-grain ratio, grain filling rate of the small-or middle-panicle type hybrid rice combinations was very high, so that the grain filling plumpness and the head milled rice was not high; while under the high level of nitrogen application, the leaf-grain ratio and grain filling rate was increased further, so that the grain unit weight and the head milled rice was decreased further. It suggests that the number of spikelets per panicle below 194 under seedlings were transplanted at 4.5-leaf stage, with a planting density of 19.98 cm×26.64 cm and 2 seedlings per hill. A total of 150 kg N per ha was applied during the entire growth period. This was an index for selecting variety of good quality rice in southern region of Sichuan Province, China.

    Comparison of the Chemical Components and Flavor Comparison of Chemical Components and Quality in Flue-cured Tobacco Population of Different Generations
    JIAO Fang-chan1, XU Zi-cheng2, LU Xiu-ping1, ZHENG Cong2,3, XIAO Bing-guang1, LI
    1, 1(1):  115-121.  DOI: 10.3969/j.issn.1008-0864.2012.03.18.
    Asbtract ( )   PDF (1145KB) ( )  
    Related Articles | Metrics

    The chemical components and sensory qualities of different generation flue-cured tobacco population in the breeding process of recurrent selection were studied, so as to provide theoretical basis for high aroma flue-cured tobacco breeding. The result indicated that compared with the flue-cured tobacco original parental population, the carbohydrates (reducing sugar, total sugar and starch) contents in the C1 flue-cured tobacco population decreased 28.79%, 12.53% and 52.32%, respectively. And the nitrogenous compounds (nicotine, total nitrogen and protein) contents increased 26.37%, 23.67% and 23.11%, respectively. In addition to volatile acids, the chemical composition of the rest indicators between C1 population and original parental population were significantly different. The aroma quality, aroma quantity, biting taste and total score of C1 flue-cured tobacco population were obviously improved, and the rest various sensory index showed significant difference between the C1 population and original parental population. The correlation analysis indicated that the correlation between volume of aroma and chemical components in the C1 population was higher than that in original parental population, which indicated that the recurrent selection had reinforced the correlation between aroma quantity score and conventional chemical components.

    Effect of Different Fertilizers on Continuous Tobacco Cropping Rhizospheric Soil Microorganisms and Enzyme Activities
    DUAN Yu-qi1, CHEN Dong-mei2, JIN Yan1, WANG Hai-bin2, YANG Yu-hong1, YOU Chui-hu
    1, 1(1):  122-126.  DOI: 10.3969/j.issn.1008-0864.2012.03.19
    Asbtract ( )   PDF (997KB) ( )  
    Related Articles | Metrics

    Problems caused by continuous tobacco cropping were critical obstacles restricting the yield and quality of tobacco leaf. The trial was conducted in a filed, where tobacco was continuously planted for 12 years. By applying different fertilizers, we studied the effect of different fertilizers on the yield, quality and rhizospheric soil microbial biomass, enzyme activity of the rhizosphere. The results indicated that different fertilization treatments had different effects on leaf yield, soil microorganism and enzyme activity in continuous cropping, and farmyard manure application was favorable to improve the leaf yield. The result of soil microbial biomass analysis showed that microbial biomass C and N, and microbial respiration intensity ranked as farmyard manure>organic fertilizer>nitrogen fertilizer>compound fertilizer after different fertilizer treatments. Furthermore, the activity of neutral phosphatase, alkaline phosphatase, urease and sucrase in tobacco rhizospheric soil was the highest with application of organic fertilizer, and farmyard manure application had the highest acid phosphate activity. In addition, the activity of soil enzyme (polyphenol oxidase, peroxidase, catalase, dehydrogenase) involved in resistance ranked as farmyard manure>organic fertilizer>nitrogen fertilizer > compound fertilizer.

    Studies on Quality &|Disease Extent of Codnopsis pilosula(Franch.)Naff under Different Density and Fertilizing Amount
    HE Chun-yu1, ZHANG Yan-hong2, LIN Hai-ming3, Guo Ye-hong3
    1, 1(1):  127-131.  DOI: 10.3969/j.issn.1008-0864.2012.03.20
    Asbtract ( )   PDF (1150KB) ( )  
    Related Articles | Metrics

    This paper studied on the roots grade, extractum, pesticide residue, heavy metal, disease infection rate under the effects of different transplanting density and fertilizing amount, in order to get the best combinations of density and fertilizer amount. Then high quality and good commercial products will be produced.  The results showed that the first grade roots are focused on the density of 0.6 million roots/hm2. The second grade is mainly produced by the combination of 0.9 million roots/hm2 density and 360 kg/hm2 fertilizer amount. The different rate order of second grade is 0.6 million roots/hm2>0.9 million roots/hm2>0.75 million roots/hm2>1.05 million roots/hm2. The whole products are occupied by the third grade roots. All extractums are higher than the standard value, and 4 combination values are more than 70.0%. The content of heavy metals is lower than the standard. Under the same density, the maximum contents of As and Cu have been checked out from the fertilizer of 360 kg/hm2, Pb checked out from zero fertilizing, Cd almost being equal to 0.01%, but Hg changing irregularly. The rate of root disease appears reducing with the increase of fertilizer amount under the same density, and the combination of 0.75 million roots/hm2 and 360 kg/hm2 fertilizer gets the minimum 1.48%. Density and fertilizer amount have played very important roles and the excellent combinations are able to produce high quality roots. It is very important for quality and commercial characters of Codnopsis pilosula(Franch.)Naff, when the combination of density and fertilizer amount reaches an ideal level.

    Studies on Technology for Controlling Pollutants from Decentralized Duck Breeding at Islet of Baiyangdian
    LIU Xiao-ning, HU Zheng-yi, ZHU Chun-you, WANG Jin-zhi, SHI Yi-chao, HUANG Li-ju
    1, 1(1):  132-137.  DOI: 10.3969/j.issn.1008-0864.2012.03.21
    Asbtract ( )   PDF (1178KB) ( )  
    Related Articles | Metrics

    The pollutant from decentralized duck breeding in Baiyangdian is one of the major sources of its water body. This paper puts forward a controlling mode of decentralizing buck breeding at islet of Baiyangdian, including duck dung collecting system(collecting network, collecting ditch for runoff and washing water), duck dung fermenting methane and waste utilization system, terminal wastewater treatment system. The controlling demonstration engineering was established in an islet with 5 000 m2 land and raising 1 500 ducks at Wangjiazhai Village, Anxin Town, Anxin County. From April to November 2010, the  concentration of COD, TN, TP in wastewater discharged from duck breeding farm was 351 mg/L, 74.2 mg/L, 7.1 mg/L on the rain days, and 214 mg/L, 42.1 mg/L, 5.3 mg/L on non-rainy days, which was lower than allowable discharge standard value of pollutants for livestock and poultry breeding(GB 18596-2001). It could be attributed to the source-collecting 40 percent of duck manure distributed in duck recreation area, and the effective removal of pollutants in wastewater discharged from duck breeding by terminal treatment system. The biogas produced from biogas tank could also be used to meet duck farmers demand for energy in their daily life. In summary, this technology could decrease the pollutants movement from duck breeding farm to the water body. This controlling mode of decentralizing buck breeding at islet is easy, operational, and of extension value.

    Studies on Bacteriostasis of Needle Aqueous Extract of Picea Asperata in Qinghai-Tibetan Plateau
    Studies on Bacteriostasis of Needle Aqueous Extract of
    1, 1(1):  138-144.  DOI: 10.3969/j.issn.1008-0864.2012.03.22
    Asbtract ( )   PDF (1423KB) ( )  
    Related Articles | Metrics

    The antibacterial role of needle aqueous extract of Picea asperata (NAEPA) from Zoige in Qinghai-Tibetan Plateau was evaluated on 6 pathogenic bacteria, and its active compounds were investigated by gas chromatograph-mass spectrometry (GC-MS). In vitro antibacterial assay showed that NAEPA inhibited effectively both against Gram-positive and Gram-negative bacteria including Staphylococus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Hafnia alvei, with minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) being 3.8~15 and 7.5~30 mg/mL, respectively. The antibacterial compounds were stable even after being heated to 121℃ for 15 min. Antibacterial activity was the highest in acidic environment, but decreased when pH increased. The chemical composition of NAEPA assay revealed that organic acids were the main components. Benzoic acid, m-hydroxybenzoic acid, pyrocatechol, squalene and myrtenol were the main antibacterial compounds. In addition, NAEPA had antioxidant, lipid-lowering and antithrombotic compounds. Those results suggested that NAEPA might be a new potential source as natural antimicrobial agent applied in food and feed industries.

    Research Progress on Ericoid Mycorrhiza
    ZHONG Lin-fang, YU Shu-jun
    1, 1(1):  145-150.  DOI: 10.3969/j.issn.1008-0864.2012.03.23
    Asbtract ( )   PDF (1044KB) ( )  
    Related Articles | Metrics

    Rhododendron  is one of traditional flowers in China, and it has excellent ornamental and application values. Survival of rhododendron under severe natural environment is intimately related with ericoid mycorrhiza.  To carry out studies on ericoid mycorrhiza is not only of important value for rhododendron garden application, but also of great significance for species diversity conservation. This paper expounds the biodiversity of ericoid mycorrhizal fungi and its function in rhododendrons growth and development. This study will surely lay a solid foundation for further utilization and mechanism exploitation of ericoid mycorrhizal fungi.