Table of Content

15 August 2012, Volume 14 Issue 4

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Achievement, Problem and Scientific Policy of Rapeseed Industry Development in China

YIN Yan, WANG Han-zhong

2012, 14(4):  1-7.  DOI: 10.3969/j.issn.1008-0864.2012.04.01

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Rapeseed, one of the most efficient oil crops, occupies a pivotal position of oil supply in China. In the last 50 years, rapeseed industry experienced 3 stages of striding-development, from low yield to good quality and high yield, which played a key role in meeting the increasing edible oil demand in China. However, more than 60% of the edible oil consumption is relying on imports and the planting area and per unit area yield of rapeseed grounded to halt leading to a decreasing supply of self-sufficiency rate. The contradiction between supply and demand of edible oil will be more outstanding. Therefore, it is necessary to summarize the historical experience to draw up a strategy for rapeseed industry by relying on science and technology, which will enable China to go a step further in edible oil production.

Initial Exploring the Management Optimization of Industry Supply Chain of Bee Products

YAO Jun, FENG Hao-ran, LIU Jian-ping, ZHANG Yang, WU Jie

2012, 14(4):  8-12.  DOI: 10.3969/j.issn.1008-0864.2012.04.02

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There exist problems in bee industry supply chain management, such as unstable supply of raw materials and food safety. These issues are bound to affect the sustainable and healthy development of bee industry. On the basis of analyzing the status and characteristics of bee industry supply chain, this paper put forward several methods to optimize the bee industry supply chain management by strengthening enterprises participation in raw material production, emphasizing food safety as the core status in supply chain management, and improving informationization  level of supply chain management. To optimize the existing supply chain is of important significance for promoting long period, stable and rapid development of bee industry in China.

Regulation of LINE-1 Retrotransposon in Genome

ZHU Yun-feng, LI Guan-lin

2012, 14(4):  13-23.  DOI: 10.3969/j.issn.1008-0864.2012.04.03

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In the eukaryotic genomes, many repetitive DNA sequences were identified in the introns of genes and intervals between the genes, even though they are not translated into protein. Recently these sequences were characterized to participate in regulation of gene expression. Among these repetitive sequences, a retrotransposon called LINE-1, which is widely distributed in the genome, is found to play an important role in cell proliferation, differentiation and tumorigenesis through the mechanisms of affecting gene transcription, genome stability, X chromosome inactivation and genomic evolution, etc. It is known that the aberration of spatial and temporal regulation of gene contributes to tumorigenesis. This article mainly elaborated the structure feature of LINE-1 and the adjusting and control function in the genome that affect gene expression and regulate the biological activities. So the studies on LINE-1 apparently facilitate to understand the formation and development of cancer.

Research Progress on Xylose Transporters in Yeast

WANG Na1, YUAN Wen-jie1,2, BAI Feng-wu1, LI Shu-ying2

2012, 14(4):  24-30.  DOI: 10.3969/j.issn.1008-0864.2012.04.04

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Cellulosic ethanol produced by biological fermentation of agricultural and industrial residues, which has wide resources with low price, is regarded as the second generation fuel-ethanol. lignocellulosic fuel-ethanol is receiving more and more attention, owing to the fact that it does not compete for land with crops, neither for grain with human beings. Xylose is the major building blocks of hemicellulose, so its utilization is the bottleneck for realizing economical and feasible production of bio-ethanol from lignocellulose. Considerable effort has been made to introduce pentose-metabolizing pathways into Saccharomyces cerevisiae, and to optimize the native pentose phosphate pathway. But the rates of xylose consumption by such engineered strains and the ethanol yield are still lower than glucose. One of the most important reasons is the limitation of xylose from extracellular to intracellular. In other words, we have to discuss the importance of sugar transporters in xylose fermentation. In recent years, the importance of xylose transporters has been revealed, and big progress has been made. In this article, the current research on xylose transporters were reviewed and discussed.

Aquaporins and Salt Tolerance of Higher Plant

SHI Gong-yao1, WANG Yu-mei2, HUA Jin-ping1

2012, 14(4):  31-38.  DOI: 10.3969/j.issn.10080864.2012.04.05

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Aquaporins (AQPs) is one of the largest superfamily of channel proteins in plant, where they facilitate the transport of water and/or small neutral solutes or gases. A wide range of regulation properties, such as translational regulation, gating mechanism, heterotetramerization and subcellular localization, make aquaporins a large contribution to membrane hydraulic conductance, which plays an important role in controlling water absorbing and transporting in plants. Osmotic stress, ion toxicity, active oxygen and other stresses induced by salinity severely affect plant growth and development. Plant aquaporins are integrated throughout the adaptations to salinity. The functional characteristics of plant aquaporins, injury forms of salt stress on plants, and roles of aquaporins helping the plant in combating salt stress were reviewed. And the study in plant aquaporins and feasibility in improving plant salt tolerance by rational use of aquaporins were proposed.

Studies on Insect-resistant Transgenic Maize (Zea mays L.) Harboring Bt cry1Ah and cry1Ie Genes

YANG Zhao-jun1, LANG Zhi-hong1, ZHANG Jie2, SONG Fu-ping2, HE Kang-lai2, HUANG D

2012, 14(4):  39-45.  DOI: 10.3969/j.issn.1008-0864.2012.04.06

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A plant expression vector pMUHUESGM harboring modified Bt cry1Ah gene, Bt cry1Ie gene and glyphosate-tolerant 2mG2-epsps gene was constructed. The foreign genes cassette of the vector pMUHUESGM was transferred into maize (Zea mays L.) immature embryonic calli by microprojectile bombardment. Twenty-four T0 regenerated plants were obtained with 2mG2-epsps gene as a selectable marker gene and glyphosate isopropylamine salt as a screening agent. PCR analysis showed that 20 plants had the integration of cry1Ah, cry1Ie and 2mG2-epsps genes. T0 and T1 generation plants molecular detection showed that foreign genes had been not only integrated into the maize genome,but also inherited and expressed stably over generations in maize. Bioassay revealed that transgenic events had high resistance to Asia corn borer. These results provided reference for breeding of new insect-resistant maize,moreover fragment transformation improved biosafety of genetically modified organisms.

Bioinformatic Analysis, Subcellular Localization and Interaction in the E3 complex of DDB2 from Solanum lycopersicum

QIAO Mai-ju, TANG Xiao-feng, LIU Ji-kai, YU Jin-de, LIU Yong-sheng

2012, 14(4):  46-54.  DOI: 10.3969/j.issn.1008-0864.2012.04.07

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UV-induced DNA damage in plants has harmful effect on agriculture production. The UV-damaged DNA binding protein-2 （DDB2）has been identified to participate in DNA damage repair process caused by UV radiation in human, rice and Arabidopsis. DDB2 could interact with DDB1 and CUL4 to form the typical E3 ubiquitin ligase complex, which was involved in various developmental processes in plant life style, including root and shoot development, flowering control and photomorphogenesis. DDB2 gene was cloned from tomato and constructed to the DDB2-YFP fusion expression vector. Through semi-quantitive PCR analysis, DDB2 was found to be up-regulated upon UV irradiation in wild type tomato. Bioinformatic analysis showed DDB2 had two WD-40 repeats and one DWD box conserved domain. DDB2-YFP fusion protein was introduced into tomato and revealed that DDB2 was targeted to tomato nuclei exclusively. Yeast two-hybrid assays were performed and confirmed DDB1 could interact with DDB2 and CUL4, supporting the formation that DDB1 acted as a bridge to form the typical CUL4-DDB1-DDB2 complex.

Studies on HCF243 Participates in Regulation of Arabidopsis PSⅡ Complex Stabilization

ZUO Ran, XU Mei-ling, ZHANG Dong-yuan

2012, 14(4):  55-60.  DOI: 10.3969/j.issn.1008-0864.2012.04.08

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Numerous nuclear encoded auxiliary factors have been identified to play key regulation and assisting roles in the dynamics of photo-system Ⅱ (PSⅡ) complex. Previous research indicated that HCF243, act as a cofactor, could promote the subsequent interaction between D1 and other PSⅡ subunits, and maintain the stabilization of D1 within PSⅡ complex. In order to explore the roles of HCF243 in PSⅡ under photo-inhibition, we identified the T-DNA insertion site of the hcf243 mutant by TAIL-PCR and analyzed the expression profiles of the gene (At3g15095) by semi-quantitative RT-PCR. Results showed that the transcript abundance of Hcf243 were the highest in leaf and gradually up-regulated as the leaf developed. The transcript abundance also varied under different stresses. Pulse labeling experiments indicated that the assembly rate of D1 protein into PSⅡ complex in mutant hcf243 was much slower than in wild type. Furthermore, the immunoblot analysis of protein complexes revealed that D1 protein decreased severely in hcf243 under photo-inhibition compared to the wild type, which might contribute to the decelerated assembly rate of PSⅡ complex. It could be inferred from our research that HCF243, as a cofactor, was essential for the turnover, especially degradation of D1 protein in Arabidopsis PSⅡ complex.

Incidence of Sweet Cherry Crinkle Leaf Disease in Beijing and its Influence to Fruit Production and Quality

WANG Jing, LU Yue-bang, ZHANG Xiao-ming, YAN Guo-hua, ZHOU Yu, ZHANG Kai-chun

2012, 14(4):  61-65.  DOI: 10.3969/j.issn.1008-0864.2012.04.09

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Recently, sweet cherry crinkle leaf disease seriously hurts production and qualities of sweet cherry. However, detailed disease observations and effective prevention and control measures have not been reported in china. Two years successive and systematic investigations were caried  on 248 adult cherry variety “Hongdeng” in a sweet cherry orchard near the Fragrant Hill, Beijing. We find that sweet cherry crinkle leaf disease mainly affects leaf shape, fruit development shape, fruit quality and production. Leaves become rough, narrow and with abnormal distorted edge. Fruits develop slowly with abnormal fruit and crinkled leaf being in the same branch. The crinkle leaf disease has made the fruits shorter, thinner and lighter. Their maturing date is postponed and yields reduced remarkably. Crinkle leaf disease can be found in several leaves, a branch or the whole tree. The incidence situation is different every year. However, the serious disease affected trees show stable performance. The best periods for observing crinkle leaf disease are from frondescing stage to pithardening stage in spring and in autumn, respectively. Grafting test was conducted in serious crinkle leaf disease affected trees, but no crinkle leaf disease is observed in scion.

First Exploration on Cloning and Function Analysis of BgERF from Bruguiera gymnorhiza

ZHAO Yang-min1,2, PANG Jun-feng2, ZHANG Zhan-lu3, JI Peng3, WEI Ya-hui1, WU Yan-

2012, 14(4):  66-72.  DOI: 10.3969/j.issn.1008-0864.2012.04.10

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In this study, the full-length (1 870 bp) cDNA sequence of an ERF transcription factor genes, BgERF (GenBank accession No: GU593720) was isolated from Bruguiera gymnorhiza living in Shenzhen Mangrove Nature Reserves, using RACE-PCR technology. Bioinformatics analysis showed that BgERF had a complete open reading frame of 1 425 bp, encoding a protein of 475 amino acids. Three-dimensional structure was composed of a typical α-helix and 3 β-turn, with a conservative AP2/EREBP domain, and the 14th amino acid of AP2/EREBP domain is alanine (A). The 19th is aspartic acid (D), belongs to the ERF transcription factor family of proteins in AP2/EREBPF; the NCBI-BLAST homology sequence comparison and phylogenetic tree analysis showed that the BgERF had high homology in amino acid conserved regions with ERFs from most other plants like carrot, Arabidopsis, rice, pepper, tomato, soybean, potato., Gossypiumhirsutum L. This study also constructed a highly efficient plant expression vector PBI121-35s-BgERF, so as to lay a foundation for its functional and salt resistance mechanism research analysis.

Selection and Adaptation: |Molecular Evolution and Functional Divergence of Orchid Chalcone Synthase Gene Family Members

HAN Ying-ying1, MING Feng2

2012, 14(4):  73-80.  DOI: 10.3969/j.issn.1008-0864.2012.04.11

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This paper reviews the achievements made at home and abroad on molecular evolution of plant CHS genes during he past 15 years. Combining with our study results on Orchid CHS genes, this paper sums up the molecular evolution and functional divergence of Orchid CHS genes and reveals the ubiquitously existed phenomenon and mechanism of sequence evolution accompanied by functional differentiation of CHS in plant kingdom. The relationship between molecular evolution of CHS, biological evolution and natural selection is also discussed.

Isolation, Identification of Pullulanase Producing Strain and Optimization of its Fermentation Conditions

QIAO Yu1, DING Hong-biao1, YAN Jun-yan1, WANG Hai-yan2

2012, 14(4):  81-86.  DOI: 10.3969/j.issn.1008-0864.2012.04.12

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Pullulanase is considered as a starch debranching enzyme, and is very useful in starch industry. The present study aims at the isolation and identification of bacteria producing pullulanase from the environmental samples, and studies on the optimum growth conditions in fermentative medium. The strains producing pullulanase were obtained by using spread-plate method. A strain with the highest pullulanase activity was identified according to its morphological, physiological, biochemical and 16S rDNA sequence. A series of experiments were conducted for the optimization of fermentative medium and growth conditions. Among the isolated strain, strain H4 showed the highest pullulanse activity from the soil close to a starch processing plants. Strain H4 was identified as Exiguobacterium sp.. The optimum medium for pullulanase was consisted of 2% potato starch, 1% peptone, 0.5% yeast extract, 0.1% KH2PO4, 0.05% MgSO4·7H2O and 0.0001% FeSO4. Under the condition of initial pH 7.0, 37℃ and 200 r/min for 72 h, the yield of pullulanase could reach to 6.35 U/mL, which was 110.2% higher than the control (3.02 U/mL).

Design and Implementation of Farmland Management System Based on GIS

XU Shi-pu, CHAN Jian-lin, CAO Kai-xiong, YUAN Tao, WANG Yun-sheng

2012, 14(4):  87-92.  DOI: 10.3969/j.issn.1008-0864.2012.04.13

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According to the practical requirements of farmland, including realtime and movement on data acquisition, the paper designs and develops a farmland information management system based on GIS, which includs farmland attribute data management module, agricultural production operation management module and farming operation management module supported by GIS technology. The system database is developed by Microsoft SQL Server 2005, and the server software is developed by C#. The mobile server uses VS2005 environment and browser is developed based on XHTML and JavaScript. In the farmland data management module, the system has the functions of data acquisition, data export, attribute query, data statistical analysis and layer editor. With the support of commercial communications, operators can obtain information of farmland data or farm work by using smart phone or PDA in this system and then send the data to the server. Managers can use remote operation in their office. The system can provide more systematic and effective services for plots with research, teaching, production and other functions.

Research Status on Phytoremediation of Copper Contaminated Soil with Hyperaccumulator

JIN Yong1,2, FU Qing-ling1, ZHENG Jin2, KANG Wei3, LIU Yong-hong1, HU Hong-qing1

2012, 14(4):  93-100.  DOI: 10.3969/j.issn.10080864.2012.04.14

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Cu is an essential micronutrient for plant, but Cu excess will seriously affect the plant growth and animal health. With the development of modern agriculture and industry, Cu has become one of the main pollution elements in China's heavy metal contaminated soil. As a research focus and frontier field in remediation of environmental contamination, phytoremediation is a green technology which has various advantages and is incomparable to other traditional soil contamination remediation methods. In recent years, studies on phytoremediation of Cu polluted soil in china are developing rapidly. Elsholtzia splendens, Commelina communis, Ricinus communis and other copper hyper-accumulators have been discovered. Meanwhile, organic acids and microbial resistance to Cu can also enhance the repair of hyper-accumulators. This article reviews the general principles of phytoremediation, introduces the effect of hyper-accumulators on Cu enrichment, which has attracted more study focus at present. It also expounds hyper-accumulators tolerance to Cu, its detoxification mechanism and other factors-invigoration effect.

Effects of Fenlong Cultivation on Yield and Quality of Dryland Crops

WEI Ben-hui, SHEN Zhang-you, GAN Xiu-qin, LIU Bin, LU Liu-ying, HU Po, LI Yan-yi

2012, 14(4):  101-105.  DOI: 10.3969/j.issn.1008-0864.2012.04.15.

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“Fenlong cultivation” is a new farming method. Compared with the conventional cultivation (tractor preparation of soil, as CK), by Fenlong planting the yields of maize, peanut, soybean, sugarcane and mulberry   increased 25.60%, 13.78%, 10.00%, 21.91%, 54.81%, respectively; and the soybean protein increased 12%, the sucrose content in sugarcane increased 3.81%, the quantity and length of crop root system increased correspondingly, and vertical distribution moved down, horizontal distribution expanded. All these indicated that yield increase and quality improvement of these dryland crops was closely related with Fenlong cultivation, which promoted the development of crop root system. The paper also studies on the scientificalness and feasibility of this new farming method.

Effects of Drought Stress on Peanut PnAG1 Gene Expression and Physiological Parameters

ZHOU Xi1,2, ZHOU Hao1,3, SHAN Shi-hua1, LI Lin2, YANG Chen1,4, LIU Yu1,3, YAN Ca

2012, 14(4):  106-114.  DOI: 10.3969/j.issn.1008-0864.2012.04.16.

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In this study, we studied on the pertinence between PnAG1 gene which relevant to NBS-LRR with drought stress, we compared the changes in peanut main defense enzyme activity and the dynamics of PnAG1 gene expression under different degrees of drought stress. After drought stress, leaf peroxidase (POD) activity, Catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione oxidoreductase (GR) activity, malondialdehyde (MDA) content and PnAG1 Gene expression change dynamic of 2 peanut varieties during different periods were measured. The results showed that after drought stress the changes in POD、GR、MDA of 2 varieties showed a tendency of first rising then declined. The SOD activity was rising continuously, while the changes in  PnAG1 gene expression also showed a trend of first decline then rise, similar to the changes in defensive enzyme activity. Therefore, conclusion was drew that the PnAG1 genes might be interrelated with the plant defense system on the basis of the disease resistance.

Influence of Fencing on Vegetation and Soil Seed Bank in Xilamuren Grassland

ZHANG Rui-qiang1*, GAO Tian-ming1*, ZHANG Jing2

2012, 14(4):  115-120.  DOI: 10.3969/j.issn.1008-0864.2012.04.17

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Fencing is conductive to damaged grassland ecosystem restoration. 5 years fencing experiment from 2007 to 2011 was carried out to continuously monitor the sample fields vegetation and soil seed bank, and to study on the comprehensive restoration effect of fencing on the vegetation and soil seed bank in Xilamuren degraded grassland, Inner Mongolia. The results showed as follows: ① Biomass, coverage and height of fencing plot are notably more than that of heavy grazing plot. Stipa krylovii and Leymus chinesis are dominant and therefore the lower the Shannon-Wiener biodiversity index is. The production of heavy grazing plot is less than the fencing plot. Aritimisia frigida, Convolvulus ammannii and Salsols collina etc. are dominant and therefore the larger the Shannon-Wiener biodiversity index is. ②Precipitation decides the situation of soil seed bank. Fencing promotes the cumulative of all plant seeds in soil. There is a big difference in soil seed bank between fencing plot and heavy grazing plot, the former Stipa krylovii is dominant, the latter Aritimisia frigida, Convolvulus ammannii and Salsols collina et al. are dominant. Fencing can promote production performance of grassland and accumulation of soil seed bank, but fencing for long-term is not conductive to grassland biodiversity. Moderate or light grazing can promote grassland biodiversity.

Screening and Preliminary Identification of Effective and Affinity Strains of Growth-Promoting Rhizobacteria from Curcuma wenyujin root

LIU Xiang-liang1, ZHOU Xiao-lei1, HE Wen-fei2, TANG Xin-yun1, JIANG Cheng-xi2

2012, 14(4):  121-127.  DOI: 10.3969/j.issn.1008-0864.2012.04.18.

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Lectin is purified from Wenyunjin (Curcuma wenyujin) and used as a tool to screen effective and affinity plant growthpromoting rhizobacteria strains, which can react with Wenyunjin lectin. Lectin can also be purified form caudex of Curcuma wenyujin by Chitin column chromatography, and the specific concentration and specific activity of lectin are 0.074 mg/mL and 8 648.65 HU/mg, respectively. Forty four strains were isolated from Wenyunjin caudex and 26 strains could agglutinate positively with the wenyunjin lectin. Among these 26 PGPR strains, 13 had high siderophore production, accounting for 50% of Wenyunjin PGPR strains isolated. Strains WEK0101 and WEP0500 are Pseudomonas spp.; WE02 and WEP0200 Lactobacillus spp.; WEN0101 Azotobacter spp.; and WEK0102 is Micrococcus sp. These strains might be further evaluated for their high potential of specific bio-fertilizer of wenyunjin. Six strains reacting positively with Wenyunjin lectin and high siderophore concentration were identified.

Molecular Epidemiology Studies on Aeromonas hydrophila Strains Isolated from Fresh Water Fish in Liaoning Province

WANG You-juan, LI Rong-feng, LI Hua, LI Qiang, XU Xiang

2012, 14(4):  128-134.  DOI: 10.3969/j.issn.1008-0864.2012.04.19

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In this paper, RS selective medium and a dual PCR were used to amplify the 16S rDNA gene and aerolysin gene of aeromonas hydrophila isolated from fresh water fish in Liaoning Province. This is a relatively large scale and detailed epidemiological investigation in Liaoning Province. Besides, susceptibility testing and virulence verification test were carried out in virulent aeromonas hydrophila strains. The results showed that there were 26 isolates bacteria which obtained 685 bp fragment (the 16S rDNA gene) among the 51 collected strains, which proved to be aeromonas hydrophila. The percentage was 50.98%. Among them, 23.99% were isolated from diseased fish with main symptom of hemorrhagic, 17.99% with main symptom of enteritis, and 9% gill-rot. Besides, 17 of them were amplified successfully the aerA gene (252 bp), suggesting they were pathogenic. Through the artificial infection and drug sensitive test, It was found that there were significant differences among 26 aeromonas hydrophila strains in virulence and drug resistance. Up to 90% of these strains were resist to pioneer V, and they also showed a relatively high resistant rate to novobiocin、erythromycin、rifampin and tetracycline, namely 56.2%, 427%, 37.5% and 311%. However, almost all of the strains were sensitive to ofloxacin、norfloxacin and gentamicin. This study would provide significant theoretical basis for building the area pathogen library and clarifying the differences among aeromonas hydrophila strains in geographical distribution, virulence and drug resistance, and then figuring out their phenotypes and genotypes.

Studies on Methods of Agrobacterium-mediated Transformation of Indica Rice (Shuhui 527)

LIU Liang-liang, TANG Wei, YU Xu, LIU Yong-sheng

2012, 14(4):  135-141.  DOI: 10.3969/j.issn.1008-0864.2012.04.20.

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The callus transformation rate of Indica rice in tissue culture is relatively poor. Especially, the establishment of highly efficient regeneration system of superior varieties and the factors which affect its transformation remain unknown. This situation restricts at certain extent the progress of Indica rice genetic engineering. The seeds of restorer line Shuhui527 were used in this study to investigate the effects of quality and physiological state of theirs mature embryo callus under different temperatures and light conditions. Meanwhile, transformation efficiency influenced by differentiation medium supplemented with different hormones and the ratio between hormones were further studied. The results showed that under light condition at 32℃，the mature embryo callus growth is the best, under N6D medium callus quality is the best after twice subcultures．The NBS medium adding 2.0 mg/L KT+0.02 mg/L  NAA+3 mg/L 6-BA+50 mg/L VC+0.5 mg/L IAA was found most suitable for Shuhui 527 genetic transformation, and the differentiation rate reached 36.42%. Using our new system, rice transcription factor OsbZIP39 was introduced into Indica rice genome by Agrobacterium-mediated transformation. Resistant plants were obtained preliminarily by PCR analysis.

Method of Using Iron-ion Chelator to Recover the Flourenscent Pigment of Pseudomonas syringase

LIU Huan-huan, JIAO Wei, QIAO Bao-ming, DONG Ning-yu, JIANG Shi-jun

2012, 14(4):  142-147.  DOI: 10.3969/j.issn.1008-0864.2012.04.21

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The fluorescent pigment secreted by Pseudomonas syringae is a principal characteristic for bacteria classification, identification, and also one of potential virulence-related factors. In this study, we determined the impact of different concentrations of minimal medium components on production of P. syringae fluorescent pigment biosynthesis; and compared the influence of several iron-ion chelators on recovering biosynthesis ability of fluorescent pigment in pseudomonas syringaes and bacterial growth. The results indicated that ① concentration of iron-ion was the most significant impact-factor, which could influence colony fluorescence of P. syringae on KB medium, while the concentration of proteose peptone, K2HPO4 and MgSO4 could not obviously affect colony fluorescence. ②The production of P. syringae fluorescent pigments could get restored on KB plates and be significantly promoted in KB liquid when iron-ion specific chelator 8-hydroxyquinoline added at the concentration of 0.05 g/L, with a slight inhibition on bacterial growth. However, this recovery by 8-hydroxyquinoline could be reversed by adding FeCl3 at concentration of 0.05 g/L. ③Other metal-ion chelators, such as citric acid, tartaric acid sodium, trisodium phosphate and ethylenediaminetetraacetic acid disodium, could not restore P. syringae colony fluorescence. Therefore, adding iron-ion specific chelator 8-hydroxyquino (0.05 g/L) to KB medium was a more practical method for restoring colony fluorescence of P. syringae.

Breeding High-yield Spinosad-producing Strain by Nitrogen Ion Implantation

GUO Wei-qun1, LUO Li-si2, LI Neng-wei1, ZHANG Xiao-lin1

2012, 14(4):  148-152.  DOI: 10.3969/j.issn.1008-0864.2012.04.22.

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Spinosad are natural macrolide compound, produced by Saccharopolyspora spinosa with aerobic fermentation. It is a new bio-pesticide with expansive foreground. In this study, low energy nitrogen ion implantation technology and rational selection were used to obtain high-yield spinosad strain. The results indicated that the survival rate curve took a “saddle” shape and 30 s×2.6×1013 ions/cm2 implanted dose was used to further mutation and selection. According to the breeding of precursor and metabolite resistance, 5 high spinosad-producing mutants were achieved, the top of which was 44% higher than the initial strain. These showed that this technology is effective for high-yield fungus breeding.