A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests

doi:10.3969/j.issn.1008-0864.2012.03.08

›› 1, Vol. 1 ›› Issue (1): 49-55.DOI: 10.3969/j.issn.1008-0864.2012.03.08

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A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests

GE Dong-hua, SU Jun-ping, SONG Ping, NANGONG Zi-yan, WANG Qin-ying

(College of Plant Protection, Agricultural University of Hebei, Hebei Baoding 071001, China)

Received:2012-01-13 Online:2012-05-11 Published:2012-03-13

一株对鳞翅目蔬菜害虫具有广谱高毒力的新Bt菌株

葛东华,苏俊平,宋萍,南宫自艳,王勤英

(河北农业大学植物保护学院, 河北保定 071001)

通讯作者: 王勤英,教授,主要从事害虫微生物防治。E-mail:wqinying@hebau.edu.cn
作者简介:葛东华,硕士研究生,研究方向为害虫微生物防治。E-mail:gdh2009095@126.com。
基金资助:
公益性农业行业项目(200903042-5)资助。

Abstract

Abstract:

Bacillus thuringiensis (Bt) strain MB-15, isolated from the soil in Hebei Province, was high toxic against Lepidoptera vegetable pests. Compared with Bt HD-1, the toxicities of Bt MB-15 against Helicoverpa armigera, Spodoptera exigua, Plutella xylostella, Spodoptera litura and Pieris rapae all were higher than that of Bt HD-1 by bioassay in lab. The supernatant of Bt MB-15 fermentation could remarkably enhance the insecticidal activity of Bt MB-15 crystal protein. The bipyramidal crystals were observed by microscopic observation in Bt MB-15 strain. SDS-PAGE analysis revealed that Bt MB-15 produced crystal proteins of 130.0 kDa and 65.0 kDa. Bt MB-15 contained cry1Ac, cry2Aa, cry1I and vip3Aa gene by PCR-RFLP analysis. The results showed that Bt MB-15 could have the potential and commercial development value against Lepidoptera vegetable pests.

Key words: Bacillus thuringiensis, vegetable pests, Lepidoptera, insecticidal activity, genotype

摘要：

从河北省土壤分离出苏云金芽胞杆菌（Bacillus thuringiensis,Bt）MB-15菌株,利用室内生物活性测定的方法,与Bt标准菌株HD-1的杀虫毒力进行比较,结果发现该菌株胞晶混合液对棉铃虫(Helicoverpa armigera)、甜菜夜蛾(Spodoptera exigua)、小菜蛾(Plutella xylostella)、斜纹夜蛾(Spodoptera litura)和菜青虫(Pieris rapae)等5种鳞翅目蔬菜害虫的毒力均高于标准菌株Bt HD-1胞晶混合液。对发酵液各组分活性的分析发现,该菌株的上清液对胞晶混合物的杀虫活性有明显的增效作用。光学显微镜下观察该菌株伴胞晶体为菱形,SDS-PAGE分析显示其伴胞晶体主要由130.0 kDa和65.0 kDa 两种晶体蛋白组成。利用PCR-RFLP对其杀虫基因型进行鉴定,结果表明该菌株含有cry1Ac、cry2Aa、cry1I和vip3Aa基因。推断该菌株是一株对鳞翅目蔬菜害虫的防治具有潜在的商业开发价值的Bt野生株。

关键词: 苏云金芽胞杆菌;蔬菜害虫;鳞翅目;杀虫活性;基因型

CLC Number:

S482.39

GE Dong-hua, SU Jun-ping, SONG Ping, NANGONG Zi-yan, WANG Qin-ying. A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests[J]. , 1, 1(1): 49-55.

葛东华,苏俊平,宋萍,南宫自艳,王勤英. 一株对鳞翅目蔬菜害虫具有广谱高毒力的新Bt菌株[J]. , 1, 1(1): 49-55.

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A Novel Bacillus thuringiensis Strain with Broad Spectrum and High Toxicity Against Lepidoptera Vegetable Pests

一株对鳞翅目蔬菜害虫具有广谱高毒力的新Bt菌株

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