Abstract:

The development of biosensors for the analysis of complex biological problems is essential both for the study of human diseases and validation of potential drug molecules. A novel application of intein-mediated protein splicing technologies has led to the development of a biosensor. Inteins have played critical roles in design of many assays and biosensors enabling efficient and precise analysis of important biological processes, such as enzyme activity, hormone receptor, vitamins and protein-protein interactions. In this paper, we review the application and development of intein-based biosensors.

Key words: intein, biosensor, diagnostic tool, hormone receptor, protein-protein interaction

摘要：

开发用于分析复杂生物学问题的生物传感器，对于研究人类疾病和发现潜在药物分子十分重要。内含肽介导的蛋白质剪接技术的应用推动了新型生物传感器发展，基于内含肽技术的生物传感器可以对酶活性、激素受体、维生素和蛋白质与蛋白质间的相互作用等一些重要的生物学过程进行高效精准的分析和研究。重点阐述基于内含肽技术的生物传感器相关研究进展，并对其进行了展望。

关键词: 内含肽;生物传感器;诊断工具;激素受体;蛋白质相互作用