Research Status and Development Trend of Rapid Detection of Agglutinin in Concanavalin A

doi:10.13304/j.nykjdb.2023.0098

Abstract

Abstract:

Concanavalin A （Con A） is mainly found in jack bean， and it is a plant lectin that can specifically bind to polysaccharides， glycoproteins and other substances. Con A has anti-nutritional and anti-pest effects， which is an important part of plant self-protection， and also the main cause of food poisoning and allergic reactions caused by eating legumes. Therefore， the detection of Con A is of great significance for legume vegetable breeding and food safety assessment. The research progress of novel rapid detection technologies for Con A was introduced in detail， including nanomaterial based biosensor， immune sensing technology， sugar sensing technology and aptamer sensing technology. Biosensors based on nanomaterials had high accuracy but lack biological specificity； the immune sensing technology had improved the detection speed of the traditional immune method but still had the problem of insufficient signal repeatability； sugar sensing technology had greatly improved the specificity and detection accuracy of hemagglutination method， but it could not distinguish lectins with the same sugar specificity. Aptamers based detection technology had excellent detection accuracy， specificity and repeatability， and unnatural aptamers could be obtained through chemical modification to further enhance the specificity， which should be the development trend of rapid detection of Con A in the future.

Key words: concanavalin A, edible safety, rapid detection, biosensor, legumes

摘要：

刀豆凝集素主要存在于刀豆中，是一种能与多糖、糖蛋白等物质发生特异性结合的植物凝集素。刀豆凝集素具有抗营养和抗虫害作用，是植物自我保护的重要环节，也是造成食用豆类食物中毒和过敏反应的主要原因。因此刀豆凝集素的检测对豆类蔬菜的育种、食用安全性评估等工作具有重要意义。详细介绍了新型刀豆凝集素快速检测技术的研究进展，包括基于纳米材料的生物传感器、免疫传感技术、糖传感技术和核酸适配体传感技术等。基于纳米材料的生物传感器具有较高的精度但是缺乏生物特异性；免疫传感技术改进了传统免疫法的检测速度但仍存在信号重复性差的问题；糖传感技术大幅提升了血凝法的特异性和检测精度，但无法区分糖特异性相同的凝集素。基于核酸适配体的检测技术具有优秀的检测精度、特异性和可重复性，还可通过化学修饰获得非天然核酸适配体进一步加强特异性，是未来刀豆凝集素快速检测的发展趋势。

关键词: 刀豆凝集素, 食用安全性, 快速检测技术, 生物传感器, 豆类蔬菜

CLC Number:

S643

Xiaoxiao ZHANG, Xiaoqian LI, Cheng ZHU, Chenze LYU. Research Status and Development Trend of Rapid Detection of Agglutinin in Concanavalin A[J]. Journal of Agricultural Science and Technology, 2024, 26(6): 214-225.

张笑笑, 李晓倩, 朱诚, 吕晨泽. 刀豆凝集素快速检测技术的研究现状及发展趋势[J]. 中国农业科技导报, 2024, 26(6): 214-225.

Figures/Tables 5

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传感器类型 Sensor type	具体方法 Concrete method	检测限 Limit of detection	线性范围 Linearity range	文献 Reference
电化学发光 ECL	石墨烯量子点 GQDs	0.16 pg·mL^-1	0.5 pg·mL^-1~1.0 ng·mL^-1	［33］
电化学发光 ECL	牛血清白蛋白-富勒烯/铂-钛-苝四羧酸二酐复合材料 BSA-C60/Pt-TiO₂-PTC composite	1.81×10^-5ng·mL^-1	1.0×10^-4~1.0×10³ ng·mL^-1	［37］
表面等离子体共振 LSPR	金纳米团簇 AuNCs	2 nmol·L^-1	10 nmol·L^-1~10 μmol·L^-1	［38］
荧光 Fluorescence	硼酸修饰碳点和荧光微球 BCDs and FMs	0.089 μg·mL^-1	0.125~12.500 μg·mL^-1	［39］
光电化学 Photoelectrochemistry	硫化银和溴氧化铋复合材料 Ag₂S and BiOBr oxide composite	0.35 pg·mL^-1	1.0×10^-3~1.0×10³ng·mL^-1	［40］
电化学发光 ECL	多壁碳纳米管配合物 Multi-walled carbon nanotube complexes	0.3 pg·mL^-1	0.5 pg·mL^-1~100.0 ng·mL^-1	［44］
荧光 Fluorescence	金纳米团簇 AuNCs	0.62 nmol·L^-1	0.01~1.00 μmol·L^-1	［45］
电化学发光 ECL	氮化硼量子点 BNQDs	0.15 pg·mL^-1	1.0 pg·mL^-1~1.0 μg·mL^-1	［46］
离子敏感的场效应晶体管 ISFET	银纳米颗粒 AgNPs	0.16 ng·mL^-1	—	［47］
电化学发光 ECL	金纳米颗粒 AuNPs	0.146 µg·mL^-1	0.19~10.00 µg·mL^-1	［50］
场效应晶体管 FET	金纳米颗粒 AuNPs	105 nmol·L^-1	—	［51］

传感器类型 Sensor type	具体方法 Concrete method	检测限 Limit of detection	线性范围 Linearity range	文献 Reference
电化学发光 ECL	石墨烯量子点 GQDs	0.16 pg·mL^-1	0.5 pg·mL^-1~1.0 ng·mL^-1	［33］
电化学发光 ECL	牛血清白蛋白-富勒烯/铂-钛-苝四羧酸二酐复合材料 BSA-C60/Pt-TiO₂-PTC composite	1.81×10^-5ng·mL^-1	1.0×10^-4~1.0×10³ ng·mL^-1	［37］
表面等离子体共振 LSPR	金纳米团簇 AuNCs	2 nmol·L^-1	10 nmol·L^-1~10 μmol·L^-1	［38］
荧光 Fluorescence	硼酸修饰碳点和荧光微球 BCDs and FMs	0.089 μg·mL^-1	0.125~12.500 μg·mL^-1	［39］
光电化学 Photoelectrochemistry	硫化银和溴氧化铋复合材料 Ag₂S and BiOBr oxide composite	0.35 pg·mL^-1	1.0×10^-3~1.0×10³ng·mL^-1	［40］
电化学发光 ECL	多壁碳纳米管配合物 Multi-walled carbon nanotube complexes	0.3 pg·mL^-1	0.5 pg·mL^-1~100.0 ng·mL^-1	［44］
荧光 Fluorescence	金纳米团簇 AuNCs	0.62 nmol·L^-1	0.01~1.00 μmol·L^-1	［45］
电化学发光 ECL	氮化硼量子点 BNQDs	0.15 pg·mL^-1	1.0 pg·mL^-1~1.0 μg·mL^-1	［46］
离子敏感的场效应晶体管 ISFET	银纳米颗粒 AgNPs	0.16 ng·mL^-1	—	［47］
电化学发光 ECL	金纳米颗粒 AuNPs	0.146 µg·mL^-1	0.19~10.00 µg·mL^-1	［50］
场效应晶体管 FET	金纳米颗粒 AuNPs	105 nmol·L^-1	—	［51］

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