Abstract:

Agrotis ypsilon is a destructive pest in agricultural production. The way to improve crop resistance against Agrotis ypsilon by genetic manipulation is available. Previously, the cry2Ab4 gene and vip3Aa11 gene were cloned from Bacillus thuringiensis. Two plant expressive vectors pCS2Ab and pCSvip3A harboring cry2Ab4 gene and vip3Aa11 gene were constructed and transferred into tobacco (Nicotiana tabacum L.) NC89 by Agrobacterium mediated method. 32 and 35 positive regenerated plants were obtained and confirmed by PCR, RT-PCR and Western blot, respectively. The insect-resistant capability in the transgenic tobacco plants with pCS2Ab and pCSvip3A vectors had also been detected by bioassay. The mortality of Agrotis ypsilon larvae fed with leaves from transgenic plants was above 82%, which was significantly more than the contrast. The insecticidal activity of transgenic tobacco harboring vip3Aa11 gene is higher than that of transgenic tobacco with cry2Ab4 gene.

Key words: cry2Ab gene, vip3A gene, transgenic tobacco, Agrotis ypsilon

摘要：

小地老虎严重危害农业生产，通过转基因技术提高作物对小地老虎的杀虫效果是一条切实可行的途径。cry2Ab4和vip3Aa11是从苏云金芽胞杆菌（Bacillus thuringiensis，Bt）中克隆到的两个杀虫基因。分别构建了pCS2Ab和pCSvip3A两种植物表达载体，利用农杆菌介导法转化烟草（Nicotiana tabacum L.）NC89，并得到32株和35株阳性转基因植株。通过对其进行RT-PCR分析、Western杂交分子检测证实两种外源基因在烟草植株中可以正常表达。人工饲喂初龄小地老虎幼虫，对两种转基因植株进行生物活性检测，结果显示饲喂转基因叶片的小地老虎幼虫死亡率在82%以上，抗虫能力比对照显著提高，转vip3A植株抗虫效果优于转cry2Ab植株。

关键词: cry2Ab基因;vip3Aa基因;转基因烟草;小地老虎