Abstract:

Making transgenic animal by SMGT is a very simple and feasible method for research and application, but combining and inner-transferring of outer origin DNA has very complicated mechanism, this article discussed about substance basic and molecular mechanism of integrate between sperm and outer origin DNA and probed into integrate mechanism of outer origin DNA in sperm genome. In addition, making method of transgenic animal was discussed on the basis of the development of theoretical research.

Key words: transgenic animal, sperm-mediated gene transfer

摘要：

利用精子载体法制备转基因动物是一种简便、可行的研究和应用方法，但精子结合并内化转运外源DNA却具有非常复杂的机制。从精子结合外源DNA的物质基础及分子机制，外源DNA在精子细胞中的存在和降解等方面展开探讨，并着重探讨了外源DNA在精子基因组中的整合机制。另外，基于理论研究的进展讨论了转基因动物的制备策略。

关键词: 转基因动物 精子载体