Abstract:

The effect of photoperiod on growth and development of Dendrobium officinale in vitro was studied under controlled environment in a closed plant factory with artificial lighting. Single explant each with 300 mg fresh mass of D. officinale were planted in five treatments with photoperiods of 6, 9, 12, 15, and 18h·d^-1 under controlled environment at air temperature of 22 ± 1℃, relative humidity of 65 ± 5%, lighting intensity of 68 ± 9 μmol · m^^-1· s^-1, and COz concentration of 800 ± 50 μmol· mol -l in photoperiock The net photosynthetic rate, chlorophyll content, dry mass, and buds in treatment of photoperiod of 12 h·d^-1were higher than that in other treatments, and resulted in greater growth and reproduction for D. officinale producction in vitro after the explants were cultured 92 days. Therefore, the photoperiod of 12 h·d^-1 for D. officinale production in vitro is suitable in tthe closed plant factory with artificial lighting.

Key words: chlorophyll content , controlled environment , photosynthetic rate , polysaceharide content

摘要：

在人工光型密闭式植物工厂的可控环境条件下，光照时间会影响铁皮石斛（Dendrobium officinale）组培苗的生长发育。将鲜重约为300mg的铁皮百斛单腋芽作为外植体在温度22±1℃、湿度65±5％、光照强度68±9μmol·m^-2·s^-2。、光期的CO2浓度800±50μmol·mol^-1。的环境条件下，设置光照时间为6h·d^-1、9h·d^-1、12h·d^-1 、15h·d^-1、18h·d^-1的5组试验区进行培育。该可控环境下培育92d后，光照时问为12h·d^-1的试验区的铁皮石斛组培苗的净光合速率和叶绿素含量较高，干重和腋芽数增加较多，表现出良好的生长与繁殖能力。因此，人工光型密闭式植物工厂内铁皮石斛组培苗的适宜光照周期为12h·d^-1。

关键词: 叶绿素含量 可控环境 光合速率 多糖含量