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Table of Content

    15 December 2007, Volume 9 Issue 6
    Grasp Every New Opportunity|Make Major Stride,Try Hard to Implement |the Ten Deeds of Science and Technology in Biological Economy
    LIU Yan-hua
    2007, 9(6):  1-6. 
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    Unprecedented opportunity and severe challenge are facing the biotechnology circle of our country. During the Eleventh Five-Year Plan period, we shalltry hard to implement the ten deeds of science and technology in biological economy including probing the mystery of life, bio-medical, bio-agriculture, bio-manufacture,bio-environmental protection, bio-resource, bio-safety, biotechnology and international cooperation in bio-science and technology, in order to realize a major stride in the domain of bio-technology, and to culture and develop biological economy.

    Developing Grassland Farming to Ensure the Security of Floodplain |in the Middle and Lower Yellow River Valley
    REN Ji-zhou, CHANG Sheng-hua
    2007, 9(6):  7-12. 
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    The effect of construction engineering harnessing of the floodplain in the lower Yellow River Valley is outstanding. But the measurers such as bio-control, adjustment of agriculture structure and the changing in land use method need to be improved vigorously. The security of flood control is still hard to satisfy the need for construction of long period, the level of people's living condition remains lower. It was suggested that under the condition of safeguarding the security of the floodplain, floodplain construction should fully display local resources potential, enhane bio-control harness and apply compensation mechanism. At the same time, basic infrastructure construction should be emphasized through dividing the functional district. Arable land farming system should be transformed to agro-grassland system, so as to change the unreasonable status of land utilization and to take the path of sustainable development, By promoting ecological emigration and reducing local population pressure, we can win double victories over ecology and economy, thus make the local people to live and work in peace and contentment , and improve the security and development of the floodplain to a new level.

    Agricultural Economic Rational Water Consumption and Strategy |Analysis for Water Saving Potential in China
    WU Pu-te, ZHA0 Xi-ning, FENG Hao, WANG Yu-bao
    2007, 9(6):  13-17. 
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    On the basis of analyzing the present status of water resources utilization in agriculture, and starting from the view point of safeguarding grain security and assuring macro comprehensive efficiency of water utilization, this paper puts forward the concept of agricultural economic rational water consumption, analyzes its connotation, raises its theoretical basis and relevant technological foundation based on rational disposition of macroscopical water resource. It also makes a preliminary analyses on realizable agricultural economic rational water consumption and the water saving potential in the f uture, so as to provide a beneficial exploration for relaxing the conflict between agriculture water utilization, industrial water utilization and ecological water utilization.

    Epigenetic Variation Associated With Plant Wide |Hybridization and Polyploidy
    LIU Bao, HA0 Shui
    2007, 9(6):  18-21. 
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    Sexual hybridization between different species or genera in plants is a common phenomenon in nature, which represents a predominant model of speciation, whereby rapid hybridization barrier may form and new species can emerge sympatrically with its parental ones. These wide hybridizations are also an effective means for crop improvement. But the mechanistic basis by which new species forms via this means remains largely elusive. Recent studies over the last several years have demonstrated that wide hybridization and subsequent genome doubling (pelyploidy) often induce an array of variations that could not be explained by the conventional genetic paradigms. A large proportion of these variations are epigenetic in nature. All hybridization and/or pelyploidy-associated epigenetic variations so far uncovered are alterations in cytosine DNA methylation. Nonetheless, it is conceivable that other epigenetic modifications like histone covalent modification and chromatin remodeling, which were known as interlaced with DNA methylation, may also subjective to modifications upon hybridization and/or pelyploidy. Further basic studies on epigenetic variations generated by wide hybridization and polyploidy may shed light on the mechanisms generating these variations, and will enhance our understanding on their evolutionary significance, as well as enable us to more effectively utilize these variations in crop breeding.

    Status and Suggestions for the Development of Soybean |Processing Industry in China
    JIANG Lian-zhou, HU Shao-xin
    2007, 9(6):  22-27. 
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    Soybean processing industry is an important processing industry in China. Effective utilization of soybean protein resources is an important measure to improve country's dietary nutrition structure, to increase protein intake and to enhance the nation's corporeity. Through analyzing the present situation of soybean processing industry at home and abroad, this article introduces new progress achieved in technology research of soybean processing industry. It also analyzes the problems existing in soybean processing industry, and provides several reasonable suggestions.

    Present Status of Rapeseed Science and Technology Innovation |System in China and Suggestion for Further Development
    LIA0 Xing, WANG Han-zhong
    2007, 9(6):  28-34. 
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    Rapeseed research distribution, scientific teams funds for research activities, R&D bases, equipment and apparatuses, and research achievements in China were analyzed in this paper. Main problems existing in Chinese rapeseed science and technology innovation system were pointed out. Suggestions for future construction of rapeseed scientific research system in China were also proposed.

    The Resistant Effect of EIF4E to Plant Virus
    LU Hang, ZHA0 Xiao-ming| BAI Xue-fang| DU Yu-guang
    2007, 9(6):  35-40. 
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    In the process of nature evolution, plants have evolved to overcome pathogenic infection, especially for virus, which relies on the plant's biosynthetic and energy-producing capabilities. Recessive resistance genes from mutations can destroy or restrict elF4E (eukaryotic translation initiation factor 4E ) required for plant virus infections, so as to prevent virus multiplication. This review introduces the mechanism of elF4E against virus infection in plants.

    Studies on the Mechanism for Implementing Qinchuan Cattle Standard
    WANG Yan-hua,HU0 Xue-xi,NIE Xing
    2007, 9(6):  41-46. 
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    Qin Chuan cattle industry plays a significant role in the animal husbandry of Shanxi province. To breed according the set standards and to implement quality control over the whole process is an effective means to improve Qinchuan cattle's market competitiveness both at home and abroad. Starting from the current situation of implementing standard mode for Qinchuan cattle, and according to a series of existing problems, such as farmers lack consciousness for standardization, the standard system is incomplete, etc. , this paper puts forward several measures as opening up a chanel for propaganda and popularize Qinchuan cattle standard, so as to further perfect 〈 Integrated Qinchuan Cattle Standard 〉, to establish an organization to coordinate the wwork for Qinchuan cattle standardization and to strengthen monitoring over the implementation of Qinchuan cattle standard, etc.

    Thoughts about Construction |Network for Agriculture of a Cooperation and Sharing Environment Monitoring
    SUN Bao-li, T0NG Cheng-feng, YANG Zheng-li
    2007, 9(6):  47-51. 
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    Considering the problems of information insufficiency and data service lagging behind time in agriculture environment monitoring, the paper puts forward a thought about constructing a cooperative and sharing network for agriculture environment monitoring. By means of innovating management consciousness and tools, this network can help to accelerate data sharing, promote cooperation between all agricultural fields, and tightly link environment monitoring, environment forecast and environment control as a whole, so as to eventually improve the efficiency and benefit of agriculture environment management. The paper also analyses the connotation and function of this network, and discusses several key issues concerning its construction.

    Thoughts about Reseach Teams Cooperation for Integrated |Control of Agricultural Tri-dimensional Pollution
    LAI Yan-ping, ZHU Li-zhi
    2007, 9(6):  52-55. 
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    Integrated control of agricultural tri-dimensional pollution is an emerging cross-discipline and a huge project involved in wide crossover fields. To make breakthrough in the field, the human resources, scientific and technical resources and technological platform conditions of related disciplines should be integrated to form an interdisciplinary, inter-departmental, inter-unit and inter-industry talent collaborative team. It analyzes the necessity, feasibility of team collaboration and demands of team building, prospects of team collaboration. And it provides relevant recommendations of team building.

    Genome-wide Comparative Phylogenetic Analysis of mrs2 |Gene Family in Arabidospsis and Rice
    XU Hua, YANG Ze-feng, GU Shi-liang
    2007, 9(6):  56-63. 
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    mrs2 (mitochondrial RNA splicing 2) genes were discovered as a suppressor of splicing defects in mitochondria. They have been shown to be a transporter for the movement of Mg^2+ in plants. This study collected the mrs2 genes that were isolated in plants and obtained their MRS2 domains. We identifieed the mrs2 genes in Arabidopsis and rice. A phylogenetic tree was constructed based on the Mrs2 proteins. Analyses of the conserved sequences of these pro- teins were conducted; and finally the expression information of these genes was searched. The results are as follows: ( 1 ) The phylogenetic analysis indicated that the feature of this family might have been in existence before the split of Arabidopsis and rice, and species-specific expansion might have contributed to this family after the split of Arabidopsis and rice. (2) MEME analysis indicated that the motifs in the Mrs2 protein were higghly conservative, and the order of these motifs in the proteins was quite similar. ( 3 ) The expressions of the mrs2 genes in Arabidopsis and rice weere different, but still keep consistency in some expressions.

    Optimization of Inducing Parameters to Express GST Fusion Protein |in Engineered Rhizobia and the Enhancement of Salt Resistance
    YU Zhi-jing, WEI Zheng-yi, XING Shao-chen, CAI Qin-an
    2007, 9(6):  64-70. 
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    Recombined plasmid pEGX-HAL1 was constructed using prokaryotic expression vector pGEX-4T-1, and transformed to Rhizobia by tri-parental mating to express fusion protein GST-HAL1. The results revealed that the salt resistance of engineered Rhizobia induced by IPTG was obviously improved, compared with blank and engineered Rhizobia without inducemeat. Under different temperatures, different inducing times and different IPTG inducement concentration, the expressing quantities of fusion protein were investigated to optimize the inducing parameters. The expressing quantities of fusion protein GST-HAL1 obviously increased under 28℃. Moreover, GST fusion protein can be induced effectively in engineered Rhizobia, and the product is up to 3.02 mg·L^-1liquuid medium, which is 39.34% of total soluble protein, under the inducing conditions of 3 h and 1.0 mmol·L^-1 IPTG concentration. The study may contribuute to bio-fertilizer production by utilizing gene engineering technology in the future.

    Synthesis of Spider Silk-protein Gene and Expression |of its Multimers in Escherichia coli
    ZH0U Pei, Z0U Zhu-Rong, CA0 Li-Juan, SU Yu
    2007, 9(6):  71-77. 
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    Spider silks, being a class of nature protein fibers with many functional properties, have large potentials in the fields of biomedical, material and military applications, which are however to be sufficiently obtained through the only pathway of genetic engineering. In this study, an artificial spider silk-protein gene, SPS of the full length of 429bp, was optimally designed and synthesized on the basis of the previously reported amino acid sequences of several spider silk proteins, in which the features of structural modules and their functional correlations were particularly concerned. This synthetic gene was sub-cloned in plasmid pET-32a to generate the expression vector pET-SPS1 which was further used as the backbone for creation of a set of expression vectors, pET-SPS (2 ~ 8 ) having different muhimers of SPS gene. Finally, all SPS vectors were expressed in E. coli strain BL21 (DE3) by IIPTG induction, and the recombinant SPS fusion proteins were purified. From our results, the synthetic spider silk protein gene SPS was verified identical to the design. All SPS fusion proteins expressed in E. coli are almost fully soluble. The vectors with I - 3 multimers of SPS could be efficiently expressed, while those of more increased multimers could only give rise to poor expression. A possible interpretation for this scenario was proposed.

    Case Study about Consumers' Recognition and Purchasing |Behavior for Certified Edible Agro-products --Taking Investigation on Consumers of Big and Medium Cities in Hubei, |Shandong and Sichuan Provinces as Examples
    WANG Fang, LI Xin, CHEN Song, QIAN Yong-zhong
    2007, 9(6):  78-83. 
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    Questionnaires were conducted for consumers in 6 big and medium cities as Wuhan and Xiaogan in Hubei province,Jinan and Tai-an in Shandong province,Chengdu and Panzhihua in Sichuan province,to investigate their purchasing behavior and recognition for certified edible agro-products. Descriptive statistical analysis and logistic computation modeling analysis were utilized to find out major factors influencing their ability purchasing certified edible agro-products. Relevant research conclusions and suggestions for policy makers were  put forward.

    Studies on Maize Digital Simulator
    ZHU Ye-ping, LI Shi-juan, YU Xiang-hong
    2007, 9(6):  84-89. 
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    Taking maize as an objective, this paper carries out digital simulation research on the relationship between crop, environment and technology. It applies systematic analysis and dynamic simulation technology to build a maize yield and quality formation simulator according to maize growth and development processes. Using the idea of component-based program design and the technologies of standard interface and module encapsulation, a digital system is developed to manage and monitor maize production, the visualized technology is applied to realize dynamic simulation for maize production. Finally, digitalize and scientization are realized for forecast, management, regulating and controlling, designing in maize production system.

    Suitable Photoperiod of Dendrobium Officinale in |vitro under Controlled Environment
    BA0 Shun-shu, HE Dong-xian, GU0 Shun-xing
    2007, 9(6):  90-94. 
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    The effect of photoperiod on growth and development of Dendrobium officinale in vitro was studied under controlled environment in a closed plant factory with artificial lighting. Single explant each with 300 mg fresh mass of D. officinale were planted in five treatments with photoperiods of 6, 9, 12, 15, and 18h·d^-1 under controlled environment at air temperature of 22 ± 1℃, relative humidity of 65 ± 5%, lighting intensity of 68 ± 9 μmol · m^^-1· s^-1, and COz concentration of 800 ± 50 μmol· mol -l in photoperiock The net photosynthetic rate, chlorophyll content, dry mass, and buds in treatment of photoperiod of 12 h·d^-1were higher than that in other treatments, and resulted in greater growth and reproduction for D. officinale producction in vitro after the explants were cultured 92 days. Therefore, the photoperiod of 12 h·d^-1 for D. officinale production in vitro is suitable in tthe closed plant factory with artificial lighting.

    Effects of Different Fertilizer Treatments on the Dry Matter |Accumulation of Winter Wheat and Vertical Distribution of Soil Nutrient
    PENG Zheng-ping, WANG Yan-qun, LIU Shu-qiao, WANG Hong, WANG Lei, XUE Shi-chuan
    2007, 9(6):  95-99. 
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    In the rich P and deficient K soil, effects of different fertilizer treatments, such as CK, NP, NK and NPK, on the dry matter accumulation of winter wheat and vertical distribution of soil nutrient were studied under field conditions. The results showed that along with plant development, dry matter accumulation increased gradually until ripening stage. During the growth period, dry matter accumulated in wheat represented NPK 〉 NK 〉 NP 〉 CK. Majoriity available N, P and K in soil distributed at the depth of 0 - 30cm. Partial available N and K can be collected in soil depth of 50 - 70 cm, then their concentration descending. Available P decreased sharply at depth of 30 cm. Along with wheat growth, available N, P and K in soil presented shape of ""S", the peak value of the former two was at flagging stage, while that of the latter, at turning green stage. In the same growth period and at same soil depth, the three soil nutrients with different treatments were higher than those of CK. Therefore, measures of enhancing crop root activity at shallow soil depth and promoting crop root's development in deep soil depth should be adopted. Thus, rich nutrients at both shallow and deep soil depths cann be fully utilized, which helped to increase crop yield and avoid environment contamination brought about by lost soil nutrients.

    Physiological and Biochemical Effects of Different Hypergravity |Treatment on Seeds of Wheat and Corn
    ZHA0 Xin, WNAG Jin-sheng
    2007, 9(6):  100-104. 
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    This experiment chooses seeds of wheat and corn as material, which were treated by 200 g· 24 h,200 g · 36 h,700 g · 12 h,700 g ·24 h,1 500 g · 4 h and 1 500 g ·12 h. Their seed's viability and morphological ind/ces ( plant height,root length) ,contents of chlorophyll and root's viability werre observed. Through comparing with CK, affect of gravity to plant was analyzed. The result showed that hypergravity treatment might influence the growth of young plant. Time and speed of hypergravity treatment are important factors. The best treatment in this trial is 1 500 g · 4 h,which was favorable to young plants, while the treatments of 700 g · 24 h could even lead the young plants to death. Comparison between wheat and corn has proofed that same treatment of hypergravity would have different effects on different plants.

    The Elementary Research on Ligno-cellulose |Saccharification under Diluted Acid
    BAI Yong-ke,CUI Hai-xin, CAI Hong-chang, LIU Qi
    2007, 9(6):  105-109. 
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    The decomposition and utilization of ligno-eellulose is of great significance in overcoming future energy crisis and environment problems. This paper simulates the main component of biomass-eellulose with pledget and the filter paper. The experiment on the ligno-eellulose saeeharifieation is carried out under different sulfur acid concentration, reaction time and reaction temperature. And we try to find the best reaction condition taking reducing sugar as target. The results indicate that the yield of reducing sugar increases with the increase of sulfur acid concentration when reaction time is 60 minutes. And it obtains the best value when reaction time increases to 80 minutes and sulfur acid concentration is 4%. Considering the cost of sulfur acid and the treatment of waste liquid ,we choose 4% sulfur acid concentration and 80 minutes of reaction time as the best experiment condition.

    Study on Adhesion Characteristics of Earthworms' Non-smooth Surface
    LI Jian-qiao, LIU Guo-min|Z0U Meng, LI Yin-wu|TIAN Ximei
    2007, 9(6):  110-114. 
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    The earthworm (Eisenia foetida) was taken as research object. A tiny adhesion testing system was used to discover the changing regulation between soil moisture content and normal adhesion force of earthworm's non-smooth surface by single factor test. Through orthogonal multinomial experiment, the iinfluence of positive pressure and unloading speed to normal adhesion force of earthworm's non-smooth surface was explored, and regress equation was esstablished. The test result showed that the normal adhesion force was increased with a parabola tendency along with the increase of soil moisture content. Comparing with steel specimen, the maximal rate of reducing adhesion was 44%. The positive pressure assumed two effective relations to normal adhesion force. The unloading speed assumed one effect to normal adhesion force. The adhesion force will increase along with the increase of positive pressure and unloading speed. This study can provide basic data for bionic research on biologic non-smooth surface and revealing bio-mimetic coupling mechanism.