Abstract:

The treatment of peeled cotton seeds by 0.1% mercuric chloride for 5 min, washed with sterile water 3~5 times, and then

immersed in 0.12 mg/mL antibacterial peptide liquid, was effective in preventing most common fungal contamination of the

cotton tissue culture, and did not effect the development of cotton roots. The using of antibacterial peptide in callus

induction stage showed no impact on cotton callus.The using of antibacterial peptide in the tobacco tissue culture showed no

impact on callus,shoots and shoots regeneration. Individual treatment by antibacterial peptide for the bacteria, the

bactericidal effect was less than that by cephalosprin, but the joint treatment of antibacterial peptide and cephalosprin

could prevent  bacterial contamination effectively. In this preliminary study antibacterial peptide could be  applied in

cotton and  tobacco tissue culture present good performances in fungal and bacterium contamination control.

Key words: tissue culture, contamination, antibacterial peptide

摘要：

经0.1%氯化汞处理5 min后,用无菌水冲洗3~5次,再用0.12 mg/mL抗菌肽溶液浸泡棉花去壳种子能有效地防止棉花组织培养中常见的真菌污染,且

对棉花根系的发育没有影响,同时在愈伤诱导阶段使用抗菌肽对棉花愈伤没有影响。在烟草组织培养中使用抗菌肽对愈伤及芽和芽生根没有影响

,单独使用抗菌肽对细菌的杀菌效果没有头孢菌素好,但是抗菌肽与头孢菌素配合使用能有效地防止细菌污染。经初步研究抗菌肽可以应用于棉

花、烟草组织培养,并对组织培养中的污染有良好的防治效果。

关键词:  组织培养;污染;抗菌肽