Abstract:

The clomiphene was extracted from compound feed by methanol.The extract was enriched and purified by MCX-SPE,and then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that cis-clomiphene and trans-clomiphene could be well seperated,the concentration of clomiphene and peak area was linear correlation between 0.5~25μg/mL,r=0.999 9,the average reclaim ratio was 85%~95%,and coefficient of variation was not exceeding 10%.The LDL of cis-clom...

Key words: compound feed, clomiphene, HPLC, SPE;

摘要：

用甲醇提取配合饲料中的氯米芬,提取液通过MCX小柱进行富集、净化,通过高效液相色谱仪进行检测,利用紫外检测器定量及二极管阵列检测器进行定性。实验表明,顺反式氯米芬分离良好,氯米芬浓度在0.5~25μg/mL范围内与峰面积呈线性相关,r=0.999 9;平均回收率为85%~95%;变异系数≤10%;反式氯米芬检测限为0.3 mg/kg,顺式氯米芬检测限为0.5 mg/kg;常用抗雌激素制剂在该条件下对氯米芬的测定均无干扰。

关键词: 配合饲料 氯米芬 高效液相色谱仪 固相萃取