Table of Content

30 November 2008, Volume 10 Issue S2

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Residue Determination of Organic Arsenic in Pig Feeds Using High Performance Liquid Chromatography

CHEN Dong-mei|TAO Yan-fei|WANG Yu-lian|LIU Zhen-li|YUAN Zong-hui

2008, 10(S2):  1-4. 

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Organic arsenic are widely used as medicinal feed additives as antibacterial growth promoters for pig and chicken,which has good effect on producing eggs and keeping fair of pig.But due to their side-effect,there is an urgent need to develop comprehensive control measures to monitor organic arsenic.A high performance liquid chromatography method with UV detection has been established for simultaneous determination of four kinds of organic arsenic in pig feeds,including arsanilic acid,roxarsone,nitarsone and...

Determination of Total Arsenic in Feed

LI Sheng|LI Hong|YANG Han-qing

2008, 10(S2):  5-9. 

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The different pretreatment methods and measure methods of total arsenic in feed were studied.The result showed that mixed acid digestion method had better reproducibility and accuracy when testing total arsenic in compoand feed,concentrated feed and premix digestion.Atomic fluorescence spectrometry of hydrid reaction method had high sensitivity,high reclaim ratio and low detection limit(3.6×10-11 g/mL) with advantages of lower pollution and simple operation.

Determination of Trace Selenium in Feed Using Platform Graphite Furnace Atomic Absorption Spectrometry Linking Microwave Digestion

SHANG Jun|PAN Juan|WU Jian-ping

2008, 10(S2):  10-14. 

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Conditions of platform graphite furnace atomic absorption spectrometry linking microwave digestion were determined by optimizing solvent and conditons of microwave digestion.The results showed that HNO3+HClO4+H2O2 was the best solvent of microwave digestion.The solvent could eliminate the effect of matrix in presence of matrix modifier Mg(NO3)2 and Pd(NO3)2.The concentration of selenium and peak area was linear correlation between 5~100 ng/mL(r=0.999
2).Detection limit was 0.53 ng/mL,and the reclaim ratio w...

Determination of Selenium in Feed Using Atomic Fluorescence Spectrometry Linking Microwave Digestion

WU Jin-xiao|ZHAO Ping-wei

2008, 10(S2):  15-18. 

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The samples were digested by the microwave,the potassium ferricyanide was used as the screening agent and atomic fluorescence spectrometry(AFS) was applied to determine the selenium in feed.The result showed that the concentration of selenium and peak area was linear correlation in the range of 0.0~10ng/mL(r=0.999 7),the reclaim ratio of spiked samples was 95.2%~106.0%,the coefficient of variation was in the range of 3.5%~4.2%.The method was simple,sensitive,accurate and reproducible,it could be applied fo...

Determination of Selenium in Feed Using Atomic Fluorescence Spectrometry

DING Zai-liang|ZHANG Li|LIU Hong-yun|LIU Fa-quan

2008, 10(S2):  19-21. 

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The content of selenium in feed was determined using atomic fluorescence spectromety method.The result showed that no significant difference between the constructed method and national standard method.The pertreatment time of the method was shortened greatly.The concentration of sample and peak area were linear correlation between 0.01~0.05 μg/mL,and the reclaim ratio was 97.3%.

Determination of Glucocorticoids Rresidues in Pig Urine by UPLC-MS/MS

BAN Fu-guo|JIA Zhen-min|WU Ning-peng|SONG Zhi-chao

2008, 10(S2):  22-26. 

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A method for determination of glucocorticoids residues in pig urine by UPLC-MS/MS was developed.The samples were deconjugated with β-glucuronidase/ arylsulfatase enzyme,and extracted by ethyl acetate,followed by clean-up with a dual HLB and aminopropyl cartridge.The analytes were quantified by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry(UPLC-MS/MS) operating in the negative ion mode.The assay for 9 glucocorticoids was linear over the range of 2~100 ng/mL with a h...

Determination of Nine Quinolones in Premix Using High Performance Liquid Chromatography

SHI Xing-fen|REN Yu-qin|CHEN Yong|WU Wang-jun|ZHOU Feng-chao

2008, 10(S2):  27-31. 

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An efficient Liquid chromatography-fluorescence detection method for determination of nine kinds of quinolones in compound premix was established.The method is simple,rapid,accurate,sensitive and reproducible.The concentration of nine kinds of quinolines and peak area was linear correlation between 0.05~4 μg/mL,correlation coefficient r was above 0.999 9.Average reclaim ratio was above 80% and coefficient of variation was not exceeding 7%.

Determination of Furazolidone,Furaltadone,Nitrofurazone,Nitrofurantoin in Feed Using HPLC

CAO Ying|ZHANG Wen-gang|HUANG Shi-xin

2008, 10(S2):  32-34. 

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A HPLC method for determination of furazolidone,furaltadone,nitrofurazone and nitrofurantoin in feed was established.The HPLC apparatus was assembled Waters Sunfire C18 column(150 mm×2.1 mm,3.5 μm),detection wavelength was at 365 nm.Aacetonitril and 0.2% formic acid in water was used as mobile phase with a flow rate of 0.2 mL/min.The linearity range of all nitrofran drugs was 0.1~10 μg/mL.The reclaim ratio was 75%~90%.The variation coefficient was 3.74%~5.83%.The limit of detection was 0.5 mg/kg and the lim...

Determination of Verginamycin in Feed Using HPLC

FENG San-ling|GENG Shi-wei|CHU Rui-wu|FENG Qun-ke|JIA Shu-jing

2008, 10(S2):  35-42. 

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A HPLC method to determin the contents of verginamycin in feed was established.The sample was extracted with ethylacetate,purified by silica cartridge and HLB cartridge,and then detected with UV detector or DAD at 210 nm.The concentration of verginamycin and peak area was linear correlation between 2～500 μg/mL,the reclaim ratio was 86.3%～93.3% and RSD was 3.33%～5.37% The detection limit was 0.5 mg/kg and the limit of quantification was 2 mg/kg.The results indicated that the constructed method was accurate,r...

Determination of Oxytetracycline in Feed by HPLC

SONG Hui-min|GENG Shi-wei|FENG San-ling|CHU Rui-wu|FENG Qun-ke|JIA Shu-jing

2008, 10(S2):  43-47. 

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A HPLC method to determine the contents of oxytetracycline in feed was established.The concentration of oxytetracycline and peak area was linear correlation between 0.1~50 mg/kg,correlation coefficient r=0.999 9.The limit of detection was 0.5 mg/kg,the limit of quantification was 2 mg/kg;the reclaim ratio was 94.4%～98.6%.The results indicated that the constructed method is accurate,repeatable and reliable.

Determination of Clomiphene in Compound Feed Using HPLC

ZHAN Yu-ming|ZHAO Ming-xiao|ZHU Liang-zhi|LIU Hua-yang|LI Bin

2008, 10(S2):  48-52. 

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The clomiphene was extracted from compound feed by methanol.The extract was enriched and purified by MCX-SPE,and then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that cis-clomiphene and trans-clomiphene could be well seperated,the concentration of clomiphene and peak area was linear correlation between 0.5~25μg/mL,r=0.999 9,the average reclaim ratio was 85%~95%,and coefficient of variation was not exceeding 10%.The LDL of cis-clom...

Determination of Anastrozole in Compound Feed Using HPLC

LIANG Meng|REN Ai-li|Chen Shu-yi

2008, 10(S2):  53-56. 

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The anastrozole was extracted from compound feed by diethyl ether and dichloromethane.The extract was centrifuged and the supernatant was purified by MCX-SPE,then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that the concentration of anastrozole and peak area was linear correlation between 0.1~1 000 μg/mL,r=0.998 9.The reclaim rate was 90%~112% with cofficient of variation less than 5%.The LDL of anastrozole was 1 μg/mL.Four medici...

Determination of Raloxifene in Compound Feed

LI Jun-ling|LIU Ji-ming|LIANG Meng|ZHANG Kai-chen

2008, 10(S2):  57-62. 

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A high performance liquid chromatography(HPLC) method for detection of raloxifene in feeds was developed and validated using an ultraviolet detector.Limit of quantification(LOQ) was 0.5 mg/kg,acceptable accuracy was from 88% to 99% and RSD ≤9.3% in the range of 0.5~5 mg /kg.The presented method can meet the requirements of raloxifene analysis in compound feed without any interference of other medication.The method provides technical support for better monitering raloxifene in animal feed,controling the qual...

Determination of Lincomycin and Spectinomycin in Edible Tissues of Animal by Gas Chromatography

TAO Yan-fei|YU Gang|CHEN Dong-mei|WANG Yu-lian|YUAN Zong-hui

2008, 10(S2):  63-68. 

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Combined use of lincomycin and spectinomycin is commonly in veterinary treatment.Non-standard drug use can lead to obvious side effect due to the survival residues in animal tissues and products.It would influence not only the processing and producing of animal products,but also the health of customers.A GC method of simultaneous detecting lincomycin and spectinomycin was established and validated in all animal edible tissues to a low concentration(limit of quantitation) of 30 μg/kg for lincomycin or 40 μg/...

Development of an ELISA Method for Detection of Five Derivatives of Quinoxaline-1,4-dioxide in Porcine Feeds

ZHAO Chun-bao|PENG Da-peng|TAO Yan-fei|CHEN Dong-mei|YUAN Zong-hui

2008, 10(S2):  69-75. 

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Olaquindox,carbadox and other derivatives of quinoxaline-1,4-dioxide can be accumulated in animal tissues and lead to cancer,abnormality and mutation when they were used in animal husbandry as feed additives.They have been banned by WHO,FAO and EU.Maximum residues limits(MRL) for animal muscle and liver tissues were also set up in China.So it is necessary to monitor the compounds used in feeds.An ELISA method was established to detect derivatives of  quinoxaline-1,4-dioxide.Concentrations of OVA-QCA,antibody...

Detection of Dovine and Sheep Derived Materials in Feed by Two PCR Methods

JIN Ling-yan|GU Xin|CAI Jin-hua|LIU Ya-ni

2008, 10(S2):  76-80. 

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Two polymerase chain reaction methods for the detection of bovine and sheep derived materials in feed were compared.Bovine and sheep derived materials were detected by correponding primers and probes.The result showed that 0.1%bovine and sheep derived materials could be detected by PCR method,and 0.01% bovine and sheep derived materials could be detected by realtime PCR method.

Methods for Detection of Genetically Modified Components in Soybean Meal

LIANG Ke-hong|LI Jun|LI Jun-guo|QIN Yu-chang

2008, 10(S2):  81-86. 

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Detecting methods for transgenic soybean meal and soybean raw materials through DNA and protein were carried out.The primers for endogenous gene lectin of transgenic soybean meal,CaMV35S promoter gene,NOS terminator and Cp4-epsps gene were designed and synthesized.The detection sensitivity of genetically modified protein and soybean meal with ELISA methods can be reached 0.25 percent,but the ELISA is not suitable for genetically modified material testing from processed products.The hybrid transgenic soybean...

Study of Determination of Urea in Feed

WU Jin-feng|GUO Zhi-gang|HAO Mei-yan

2008, 10(S2):  87-93. 

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A method for determination of urea in feed was estabilished.Urea was extracted by potassium hexacyanoferrate and zinc acetate,determined by colorimetry.The linear range of the method was among 0.1~10 mg/mL,with correlation coefficient was 0.999 9.The concentration limit of quantity was 0.1%.In order to validate accuracy and precision of the method,several kinds of reference feeds such as cow concentrate supplement,sheep concentrate supplement,cow concentrate,sheep concentrate and beef concentrate,were prepa...

Determination and Analysis of Melamine in Feed Using GC-MS Method

WANG Biao|WANG Deng-lin

2008, 10(S2):  94-97. 

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Melamine in feed was determined by GC-MS method.Melamine in feed was extracted and purified from feed sample.After HPLC determination and derivatization,the melamine in feed was analyzed by CG-MS method and quantified by external standard method.A total of sampling of 420 pet feeds were checked.After test with screening kit,26 samples were suspected positive by HPLC detection,17 among them were qualitatively excluded by CG-MS method.The experiments showed that positive rate of HPLC was 6.1%,GC-MS confirmed ...

Determination of Ammonia-N in Silage by Flow Injection Analysis

FAN Zhi-ying1|CHEN Jin-hui2|XIAO Jing-ze2|ZHAO Ping2

2008, 10(S2):  98-100. 

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Ammonia-N in silage was determined by flow injection analysis.The detection limit was 1.99 μg/L,correlation efficient r was more than 0.999,the RSD was 1.03% and the reclaim ratio was 96%～108%.The method has the characteristics of simplicity,sensitivity and good correlation.It is suitable to be used for determination of ammonia-N in silage with large quantity of samples.

Study of the ELISA Kit for Detecting Aflatomin B_1 in Feed

WANG Xiong1|DONG Ying-chao2|Guo Qi1|Chen Bing-jun1

2008, 10(S2):  101-105. 

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A simple,rapid,sensitive and accurate ELISA method was developed to be widely used in detection of aflatomin B1 contamination in feed.Based on anti-aflatoxin B1 monoclonal antibody and indirect competition ELISA method,a quick afltoxin detection ELISA kit was successfully produced.The detection limit of aflatomin B1 was 0.05 ng/mL,the linear range was 0.05～2.0 ng/mL.Adding-recovery experiments were done in 3 levels for 3 kinds of feeds,the results showed that the recovery rates were among 79.5%~106.2%.

Study Advancement of Biosensing and Molecular Signaling Techniques

TAN Zhong-yang1|2|JIANG Xiao-ping2|YI Li-ping3|JIANG Jian-hui1|SHEN Guo-li1

2008, 10(S2):  106-108. 

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Aptamer biosensing technology,engineering of nano-sensory interfaces and devices,and designing of molecular recognition components are the hot spots in the field of biosensing molecular detection techniques.This paper simply described these new technologies of biosensing and molecular signaling.

Evaluation of Kit Detecting Veterinary Drug Residues

FU Hui-ming|GUO Zhan-qi|WANG Cui-ying|WANG Biao|DING Xing-li|YANG Hong-yan

2008, 10(S2):  109-111. 

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ELISA kit is a new veterinary drug residues detection product.Compared with instrument analysis,detection by ELISA kit is quicke,simple,accurate,and highly sensitive.It takes only 1.5 hours to complete the operation.ELISA analysis can minimize operation error and working strength maximatically.But usually some technique index marked on the kit is far different from the examination results,there would be of great chance to emerge false positive results.It is necessary to determin the concrete contents and sp...

Safeguarding the Sustainable Development of Stockbreeding and Aquaculture Through Quality Surveillance for Feed and Livestock Products

WU Li-long|DENG Fang|CHEN Fu-hua|TAN Mei-ying|CAO Feng

2008, 10(S2):  112-117. 

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129 batches of feed samples were randomly examined for quality security surveillance in Hu nan province in the first half of 2008.98.7% of samples from the manufacture procedure accorded with the criterion,which was higher than those from the marketing for 6.5%.During manufacture procedure,the passing rate of both feed additive and concentrated/compound feed reached the standard(100%),which was higher than that of premix for 4%.But for the samples from the marketing,the eligible proportion for the feed addi...

Strengthen Feed Supervision to Ensure Feed Safety

HE Ya-xiong|LIU Wei-hua|XIE Rong-guo

2008, 10(S2):  118-120. 

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Significance of feed supervision was described from the angle of feed quality and safety.A series working suggestions were proposed to strengthen feed safety supervision,to improve the research and development of standardization of feed quality,to raise the overall level of testing and detection institutions of feed product quality,to pay attention to the feed supervisory management and the construction of feed standard system and feed detection system.Concrete measures to ensure safety of feed quality and ...