Abstract:

A simple,rapid,sensitive and accurate ELISA method was developed to be widely used in detection of aflatomin B1 contamination in feed.Based on anti-aflatoxin B1 monoclonal antibody and indirect competition ELISA method,a quick afltoxin detection ELISA kit was successfully produced.The detection limit of aflatomin B1 was 0.05 ng/mL,the linear range was 0.05～2.0 ng/mL.Adding-recovery experiments were done in 3 levels for 3 kinds of feeds,the results showed that the recovery rates were among 79.5%~106.2%.

Key words: aflatomin B1, ELISA, monoclonal antibody;

摘要：

建立一种简单、快速、灵敏、准确的黄曲霉毒素B1的ELISA检测方法,并使其在饲料检验中广泛应用。在生产抗黄曲霉毒素B1单克隆抗体基础上,利用间接竞争ELISA方法,研制出饲料中黄曲霉毒素B1快速检测试剂盒。该试剂盒对黄曲霉毒素B1最低检出浓度为0.05 ng/mL,标准曲线的线性范围为0.05~2.00ng/mL;对3种饲料做3个加标水平添加回收率试验,回收率在79.5%~106.2%之间。

关键词: 黄曲霉毒素B1 酶联免疫吸附试验 单克隆抗体