Initial Studies on Isolation of a Thermophilic Cellulose-degrading 
Bacteria and its Enzymology Characteristics

›› 2009, Vol. 11 ›› Issue (1): 124-128.

Initial Studies on Isolation of a Thermophilic Cellulose-degrading
Bacteria and its Enzymology Characteristics

QU Xiao-shuang1,2, DUN Bao-qing1, GUO Ming-ming1,2, SONG Li-li1,3, XU Xiu-hong2,
Zhang Bao-ming1, LU Ming1, Li Gui-ying1

（1.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Biomass Energy Research
Center, Chinese Academy of Agricultural Sciences, Beijing 100081; |2.College of Life Sciences, Northeast Agricultural University,
Harbin 150030|3. Northwest |A &|F University, Shannxi Yangling 712100, China）

Received:2008-10-30 Revised:2008-11-12 Online:2009-02-15 Published:2009-07-29

一株嗜热纤维素分解菌的分离及其酶学性质的初探

曲小爽1,2,顿宝庆1,郭明鸣1,2,宋立立1,3,许修宏2,
张保明1,路明1,李桂英1

（1.中国农业科学院作物科学研究所,国家农作物基因资源与基因改良国家重大科学工程,中国农业科学
院生物质能源研究中心, 北京 100081|2.东北农业大学生命科学学院, 哈尔滨 150030；
3.西北农林科技大学, 陕西杨凌 712100）

通讯作者: 李桂英,研究员,从事生物质能源研究。E-mail:liguiying@caas.net.cn。顿宝庆,助理研究员,从事能源和环境微生物研究。E-mail:baoqingdun9@hotmail.com
作者简介:曲小爽,硕士研究生,研究方向为微生物学。E-mail：xiaoshuang78611@163.com。
基金资助:
“十一五”国家科技支撑计划项目（2006BAD07A04）;农业部农村能源综合建设项目（2130138-0-08）;中央级公益性科研院所基本科研业务费专项资金（2060302-13）资助。

Abstract

Abstract:

WQ-50 was isolated from decayed wood and soil near hot spring in Hainan. It was identified as Bacilluscirculans by preliminary physiological and biochemical characteristics and genetic analysis. When fermented by CMC-Na culture medium, strain WQ-50 strain could grow well under PH 6 and 55℃. Under this condition, when fermented by zymolytic culture medium with alkalized pretreated corn stalk powder as the sole carbon source, enzyme production reached maximum after 5 days, the CMCase activity of WQ-50 is 6.8 IU/mL and FPA activiy of WQ-50 is 3.5 IU/mL.

Key words: thermophilic cellulose-degrading bacteria, screen, cellulose enzyme, identification

摘要：

利用海南热泉附近腐烂朽木及土壤,筛选分离到一株具有较高纤维素酶活性的纤维素分解菌WQ-50,经生理生化特性及遗传分析初步鉴定其为环状芽孢杆菌属菌株。当以复筛培养基发酵培养时, 该菌株在55℃、pH 6条件下生长较好。在该条件下,以经碱预处理的玉米秸秆粉为唯一碳源发酵培养5 d时产酶达到最大值,羧甲基纤维素酶活性为6.8 IU/mL,滤纸酶活性为3.5 IU/mL。

关键词: 嗜热纤维素分解菌；筛选；纤维素酶；鉴定

CLC Number:

S216.2

QU Xiao-shuang1,2, DUN Bao-qing1, GUO Ming-ming1,2, SONG Li-li1,3, XU Xiu-hong2,. Initial Studies on Isolation of a Thermophilic Cellulose-degrading
Bacteria and its Enzymology Characteristics[J]. , 2009, 11(1): 124-128.

曲小爽1,2,顿宝庆1,郭明鸣1,2,宋立立1,3,许修宏2,.
一株嗜热纤维素分解菌的分离及其酶学性质的初探[J]. , 2009, 11(1): 124-128.

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