Abstract:

Feeding trials were conducted to evaluate effects of drunken horse grass infected with

(E+) or free from (E-) endophyte, Neotyphodium gansuense, on health of rabbits. Compared

with E- grass, the content of crude fat in E+ grass increased significantly, while no

significant differences were detected in the contents of crude fiber and crude protein

between E+ and E- plants. Rabbits showed toxicity symptoms when drenched with aqueous

extract obtained from E+ grass at the dose of 10~20 mL/kg body weight. However, no

mortality was found even when drenched at the higher rate of E+ extracts. Feeding trials

showed that rabbits developed toxicity symptoms when fed with E+ grass pellet at

accumulated amount of 131.6 g/kg body weight and died when they intake E+ grass pellet up

to 350.9 g/kg body weight. The final tested rabbit mortality rate was 66.7％ during the

feeding period. There were no toxicity symptoms in any rabbits fed with E- plants in the

forms of either aqueous or fodder pellet, which indicated that the Neotyphodium endophyte

was the cause of A. inebrians toxicity to rabbits.

Key words: drunken horse grass (Achnatherum inebrians), endophyte (Neotyphodium gansuense), rabbit (Oryctolagus cuniculus), feeding

摘要：

通过饲喂试验,研究了带内生真菌(E+)和不带内生真菌(E-)的醉马草对家兔健康的影响。内生真菌的侵

染,使醉马草粗脂肪含量显著提高,粗蛋白和粗纤维含量无显著改变。按10 mL/kg体重的剂量灌服E+醉

马草水提液,家兔表现出明显的中毒症状；但累计灌服20 mL/kg体重的剂量,亦未引致死亡。平均按每

kg体重累计饲喂E+醉马草131.6 g,可引起家兔中毒；累计饲喂350.9 g可使家兔致死,死亡率达66.7%。

而灌服和饲喂E-醉马草的家兔则无异常。初步认为,醉马草对家兔的致毒机理是由内生真菌与宿主互作

产生的生物碱所致。

关键词: 醉马草；内生真菌；家兔；饲喂