Abstract:

Three genes with differential expression in bovine peimplantation embryos, namely calm3, trm112l and clip1, were gained by mRNA differential display technology. The expression of calm3, trm112l and clip1 mRNA in oocyte, 8-cells stage embryos and blastocysts were detected by RT-PCR. The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation. The expression of clip1 gene in oocytes, 8-cell stage embryos and blastocysts did not have distinct difference. The gene calm3, trm112l and clip1 were temporal-differentially expressed genes of bovine preimplantation embryos. This might be related to their participation in different physiological activities.

Key words: embryo development, mRNA differential display, embryos, gene

摘要：

利用mRNA差异显示技术成功筛选到牛早期胚胎差异表达的3个基因,即calm3、trm112l和clip1,并通过RT-PCR技术检测了各基因在卵母细胞、8细胞期和囊胚期的表达情况。结果显示,在卵母细胞和8细胞期胚胎,基因calm3和trm112l的表达量无显著性差异,而在囊胚期的表达量与前两个时期的表达量存在显著性差异,推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控, 提示该基因在卵母细胞成熟和合子基因激活中起一定作用。基因clip1在牛卵母细胞、8细胞期和囊胚期的表达量均无显著性差异,可能既存在母型调控又存在合子型调控。基因calm3、trm112l和clip1在牛早期胚胎发育过程中mRNA表达量存在时间性差异,可能与其参与不同的生理活动有关。

关键词: 胚胎发育;mRNA差显技术;胚胎;基因