›› 2009, Vol. 11 ›› Issue (S2): 60-62.

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Preliminary Analysis of Application that PEG6000 Simulated Drought Stress Treatment in Selecting Rape Resistance (Tolerance) Materials

FU Ming-lian1,2, LI Gen-ze2, YANG Qing-hui1, WANG Jing-qiao2   

  1. (1.Yunnan Agricultural University, Kunming |650201|2.Economic Research Institute, Yunnan Academy of Agricultural Sciences, |Kunming 650205, China)
  • Received:2009-10-14 Revised:2009-12-09 Online:2009-12-31 Published:2009-12-31

PEG6000模拟干旱胁迫处理在筛选油菜抗(耐)旱材料中的应用初析

符明联1,2,李根泽2,杨清辉1,王敬乔2   

  1. (1.云南农业大学, 昆明 650201|2.云南省农科院经济作物研究所, 昆明 650205)
  • 作者简介:符明联,副研究员,博士研究生,主要从事抗耐旱油菜选育及栽培技术研究。Tel:0871-5893842|E-mail:fml0871@yahoo.com.cn
  • 基金资助:

    云南省自然科学基金项目(2008CD184);云南省“十一五”科技攻关项目(2006NG12);云南现代油菜产业技术体系项目资助。

Abstract:

Using PEG 6000 concentrations of 20%, 22%, 24%, 26% simulating drought stress,  germination rate for interspecific hybrids rapeseed of different resistance (tolerance) and drought was analizied in field experiments. To show the effects that PEG6000 simulate drought strees in selecting rape resistance (tolerance) materials. The results showed that 22%~24% PEG6000 and germination time for 4~8 d are suitable for handling rape seed in selecting resistance (tolerance) rapeseed. When the concentration of PEG6000 treatment in less than 22%, the germination index can be used as an essential indicator for reference.

Key words: rapeseed, PEG6000, drought stress, germination rate

摘要:

采用20%、22%、24%、26%四种浓度的PEG6000,对大田试验中表现不同抗(耐)旱的油菜甘、芥种间杂交后代材料进行发芽试验,分析PEG6000模拟干旱胁迫发芽试验筛选油菜抗(耐)旱材料的效果。结果表明:在油菜抗(耐)旱材料筛选中,种子处理的PEG6000浓度以22%~24%为宜,发芽时间以4~8 d为宜。在PEG6000处理浓度低于22%时,发芽指数可作为一个必要的参考指标。

关键词: 油菜;PEG6000;干旱胁迫;发芽率

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