Abstract: Cyathula officinalis Kuan. is an important Chinese medicine. However, its quality has degraded significantly in the last few years, and its authenticity has been jeopardized by hybridization. To understand the accumulation of the effective components in C. officinalis, de novo next generation sequencing of C. officinalis's genome were performed using Illumina HiSeq 2000 platform using AByss. After de novo assembly of the data, a large data set composed of genomic sequences were obtained. Based on the dataset and annotation of its repetition and coding region, this paper constructed the steroid biosynthetic pathway in C. officinalis, and located the SCAR biomarkers to identify the genuineness of C. officinalis's authenticity. These results provided a foundation for the research of cyasterone biosynthetic pathway in C. officinalis, and further improvement of its quality.

Key words: Cyathula officinalis Kuan., de novo sequencing, gene annotation, cyasterone, biomaker

摘要： 川牛膝是我国重要大宗药材，但近年来品质退化严重，品种真伪混杂。为深入研究川牛膝有效成分积累的分子基础，采用第二 代测序技术利用Illumina Hi-seq 2000测序平台对川牛膝进行全基因组测序，使用AByss进行初步组装，得到一个包含大量基 因组序列信息的数据集，并对其进行重复序列及编码序列注释。在该测序结果基础上，初步预测得到川牛膝体内甾体合成途径 ，并对鉴定川牛膝真伪的SCAR分子标记进行了初步定位，为研究川牛膝主要有效成分杯苋甾酮的合成途径，改良川牛膝品质提 供了基础。

关键词: 川牛膝, 从头测序, 基因注释, 杯苋甾酮, 分子标记