Abstract:

Spinosad are natural macrolide compound, produced by Saccharopolyspora spinosa with aerobic fermentation. It is a new bio-pesticide with expansive foreground. In this study, low energy nitrogen ion implantation technology and rational selection were used to obtain high-yield spinosad strain. The results indicated that the survival rate curve took a “saddle” shape and 30 s×2.6×1013 ions/cm2 implanted dose was used to further mutation and selection. According to the breeding of precursor and metabolite resistance, 5 high spinosad-producing mutants were achieved, the top of which was 44% higher than the initial strain. These showed that this technology is effective for high-yield fungus breeding.

Key words: ion implantation, spinosad, Saccharopolyspora spinosa, mutation breeding

摘要：

多杀菌素是由刺糖多孢菌（Saccharopolyspora spinosa）发酵产生的大环内酯类化合物，是一种应用前景广阔的生物农药。采用氮离子注入方法并结合前体和自身代谢物抗性来选育多杀菌素高产菌株。结果显示利用氮离子注入诱变刺糖多孢菌，其致死率曲线呈典型的马鞍形，并确定30 s×2.6×1013 ions/cm2的注入剂量用于进一步诱变筛选。通过前体及自身代谢物抗性筛选，最终得到5株多杀菌素高产菌株，其多杀菌素的最高产量比选育前提高了44%。表明该技术对多杀菌素高产菌株的选育是一种行之有效的方法。

关键词: 离子注入；多杀菌素；刺糖多孢菌；诱变选育